Hemagglutination ability and adherence to the Buffalo green monkey kidney cell line of uropathogenic Escherichia coli

Abstract

Vranes, J. Hemagglutination ability and adherence to the Buffalo green monkey kidney cell line of uropathogenic Escherichiu coli. APMIS 105: 831-837,1997.The hemagglutination ability and adherence capacity to the Buffalo green monkey (BGM) kidney cell line of 160 wild-type strains of Eschrrichia coli isolated from bacteriuric patients were investigated. It was found that P-fimbriated E. coli strains adhered significantly better to BGM cells than did strains in which P-fimbriae were not detected, which is in accordance with the capacity of P-fimbriated strains to cause unobstructive pyelonephritis and with receptor distribution for P-fimbriae in the urinary tract. The strains which exhibited other adhesions, alone or simultaneously, showed reduced adherence to BGM cells, while non-agglutinating strains, mostly isolated from urine of patients with asymptomatic bacteriuria, did not adhere at all or adhered poorly to the utilized cell line. The BGM cells served as a good experimental model for investigation of uropathogenic E. coli adherence; because these cells originate from the upper urinary tract, they are viable and not coated with Tamm-Horsfall protein.

Charakterisierung der extended spectrum b-Lactamasen (ESBL) und Bestimmung der von Kindern isolierten uropathogenen Virulenzfaktoren von Escherichia coli (E. coli) Stämmen

“…Briefly, hemagglutination (HA) was performed using human erythrocytes and sheep, ox and guinea pig erythrocytes. Inhibition of HA was performed with P 1 antigen-containing pigeon egg white and with D-mannose (Sigma Chemical Co., St. Louis, USA), as previously described [19]. Isolates were considered to express Pfimbriae if HA was positive with human erythrocytes and inhibition of HA was positive with pigeon egg white, which was confirmed by agglutination of receptor-coated latex beads.…”

Clonal dissemination of highly virulent extended-spectrum β-lactamase-producing Escherichia coli strains isolated from the urine of non-hospitalised patients in Zagreb region

“…The expression of adhesins was defined by hemagglutination and inhibition of hemagglutination in microtiter plates, as previously described [15,16]. Briefly, hemagglutination (HA) was performed using human erythrocytes and sheep, ox and guinea pig erythrocytes.…”

“…Briefly, hemagglutination (HA) was performed using human erythrocytes and sheep, ox and guinea pig erythrocytes. Inhibition of HA was performed with P 1 antigen-containing pigeon egg white and with D-mannose (Sigma Chemical Co., St. Louis, USA), as previously described [15,17]. Isolates were considered to express P-fimbriae if HA was positive with human erythrocytes and inhibition of HA was positive with pigeon egg white, which was confirmed by agglutination of receptor-coated latex beads.…”

Virulence Characteristics of Escherichia coli Strains Causing Asymptomatic Bacteriuria

“…The expression of adhesins was defined by hemagglutination and inhibition of hemagglutination in microtiter plates, as previously described [8]. Briefly, hemagglutination (HA) was performed using human erythrocytes and sheep, ox and guinea pig erythrocytes.…”

“…Buffalo green monkey kidney (BGMK) cell line was used for the adhesion assay, which has been described previously [8]. Briefly, this continuous epithelioid cell line was incubated for 1 h with bacterial suspension of each strain studied (1.5 ϫ 10 8 bacteria/ml) and after extensive washing of the cells and indirect immunofluorescent staining, the number of adherent bacteria was determined per 40 BGMK cells.…”