Hematobiochemical changes in ehrlichiosis in dogs of Anand region, Gujarat

Bhadesiya, C. M.; Raval, S. K.

doi:10.14202/vetworld.2015.713-717

Cited by 22 publications

(36 citation statements)

References 7 publications

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“…Changes in various organs like spleen, liver, kidney and lungs were similar to earlier studies carried out by de Castro et al (2004) and Welzl et al (2001). Nevertheless, continuous exposure to tick vector and due to lack of timely treatment chronic ehrlichiosis may be encountered in stray dogs as reported by other researchers from India (Wise and Tarlinton, 2010;Abd Rani et al, 2011;Bhadesiya and Raval, 2015).…”

Section: Discussionsupporting

confidence: 82%

“…Till date, studies on CME from India have been carried out in small geographical locations (cities/towns/small states) and that too, they have been carried out either on pet or on stray dogs and somehow, majority of these studies fail to address the clinical and diagnostic challenges being presently posed by the disease (Juyal et al, 1994;Samaradni et al, 2003;Lakshmanan et al, 2006;Wise and Tarlinton, 2012;Abd Rani et al, 2011;Singla et al, 2011;Milanjeet et al, 2014;Bhadesiya and Raval, 2015;Singla et al, 2016;Kottadamane et al, 2016Kottadamane et al, , 2017. Besides these lacunae, these prevalence studies were also solely based on either commercial serology kit or on molecular based techniques and thus failed to analyse the practical relevance of both the methodologies in existing field conditions.…”

Section: Introductionmentioning

confidence: 99%

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Canine Monocytic Ehrlichiosis among working dogs of organised kennels in India: A comprehensive analyses of clinico-pathology, serological and molecular epidemiological approach

Mittal

Kundu

Chakravarti

et al. 2017

Preventive Veterinary Medicine

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Canine Monocytic Ehrlichiosis (CME) is a serious tick-borne rickettsial disease affecting canine populations globally. Besides few reports from stray and pet dogs from localised geographical regions (cities/towns/small states), a comprehensive study on prevalence of Ehrlichia canis (E. canis) among working dogs from different geo-climatic zones of India was pertinently lacking. Study of CME among these dog populations was thus carried out, encompassing clinical aspects and different diagnostic methodologies viz., microscopy, serology and molecular biology. During the two-year study period, clinical specimens from 225 cases suspected of canine ehrlichiosis were examined for clinical pathology and presence of the haemoparasites. Overall prevalence of ehrlichiosis by microscopic examination, commercial dot-ELISA kit and nested PCR assay was estimated to be 1.3%, 19.1% and 5.8%, respectively, which were found to be statistically significant by McNemar Chi squared test (p<0.05). It was also observed that possibly due to widespread use of doxycycline therapy in field, CME presently does not remain a potential threat which it uses to pose earlier. However, concurrent infections of E. canis and Babesia gibsoni were found to be mostly fatal. Keeping in view of high number of apparently healthy dogs (24) out of total positive cases (46) observed during the study, it is recommended that prevalence studies on CME should also involve screening of apparently healthy dogs. Phylogenetic analysis carried on partial sequencing of 16S rRNA of E. canis strains revealed that all of the Indian strains clustered in a single clade with other E. canis species from India and rest of the world. Molecular divergence was observed among the sequences of Brazilian and American isolates which were also included in the present study. These findings have thus opened a new paradigm for planning of pragmatic control strategies against CME.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Canine Monocytic Ehrlichiosis among working dogs of organised kennels in India: A comprehensive analyses of clinico-pathology, serological and molecular epidemiological approach

Mittal

Kundu

Chakravarti

et al. 2017

Preventive Veterinary Medicine

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“…In most instances, published data describing coagulation alterations in dogs infected by one or more vector-borne pathogens derive from case reports, cross-sectional studies, or naturally infection studies involving single pathogens [7,10,29,34]. Bleeding disorders such as epistaxis, haematuria and haemorrhagic diarrhoea has been reported in dogs with vector-borne diseases [2,6]. These disorders represent the main cause of death in dogs, especially with ehrlichiosis.…”

Section: Discussionmentioning

confidence: 99%

“…PLT count was performed with an automated blood cell counter analyser Abacus Junior Vet 5 2 . In addition, PT, APTT and FIB concentrations were measured using a semi-automated four channel microcoagulometer 3 .…”

Section: Determination Of Coagulation Profilementioning

confidence: 99%

Interpretation of Coagulation Tendency Contributing to Thrombosis in Vector-Borne Diseases (Ehrlichiosis, Anaplasmosis, Leishmaniosis, and Dirofilariasis) among Dogs

Paşa

Ural

Gültekin

2017

Acta Scientiae. Vet.

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Background: Vector-borne infectious and zoonotic diseases are an important health problem that directly affects human and animal health negatively. Results through evaluation of coagulation disorders among vector-borne diseases should be of beneficial for both human and dogs studies. According to the present author's knowledge reports regarding changes in platelet (PLT) count, prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB) and D-dimer levels in dogs naturally infected with one or more vector-borne pathogens are lacking. Therefore, the present study was aimed to detecting those parameters for relation between diagnosis and prognosis of vector-borne diseases among dogs. Materials, Methods & Results:The material of this study was 46 dogs (36 were naturally infected with vector-borne diseases and 10 were healthy) from different breed, age and of both sexes. Venous blood samples were obtained to detect PLT counts, antibodies of ehrlichiosis, anaplasmosis, borreliosis, leishmaniosis and antigens of Dirofilaria immitis. The diagnosis of vector-borne diseases was performed by using a commercial ELISA assay kits. PLT count was performed with an automated blood cell counter analyser. In addition, PT, APTT and FIB concentrations were measured using a microcoagulometer. D-dimer concentrations were determined using fluorescence immunoassay rapid quantitave test analyser. Subgroups were formed according to the number of cases and the distribution of vector-borne agent. Statistically significant decreased PLT count was found in dogs mono infected with ehrlichiosis compared to healthy dogs (P < 0.001). Changes in mean PT value in the studied animals did not show statistically significant differences among the groups (P > 0.05). APTT values in the ehrlichiosis mono infection group were significantly higher than that of the healthy control (P < 0.01). A significant increase in FIB levels were detected for ehrlichiosis mono infection and ehrlichiosis -leishmaniosis co infection versus healthy control (P < 0.001). Plasma D-dimer concentrations were found to be higher in all groups infected with vector borne diseases compared to healthy group (P < 0.001) and the differences between infected groups were not statistically significant. Discussion: Bleeding disorders such as epistaxis, haematuria and haemorrhagic diarrhoea has been reported in dogs with vector-borne diseases. These disorders represent the main cause of death in dogs. In the present study, thrombocytopenia was observed in dogs mono infected with ehrlichiosis compared to healthy. This finding is in agreement with those reported in dogs with ehrlichiosis. Plasma FIB is one of the most important factors in the coagulation cascade. In the present study, a significant difference between dogs with ehrlichiosis mono infection and ehrlichiosis -leishmaniosis co infection versus healthy controls group was observed. PT and APTT are commonly used in evaluating dogs with bleeding tendencies. In the present study, a significant differenc...

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“…Clinical manifestations of the disease often include fever, pale mucous membranes, depression, anorexia, haemorrhagia, weight loss and ocular signs (2,4,5). Laboratory findings most frequently determined are thrombocytopenia, leukopenia, anaemia, and hypergammaglobulinemia (4,6,7). All breeds may be infected with E. canis.…”

Section: Introductionmentioning

confidence: 99%

Ege Bölgesindeki Köpeklerde Monositik Ehrlichiosis: Klinik ve Hematolojik Bulgular

Tuna¹,

Bakırcı²,

Dinler³

et al. 2019

Atatürk Üniversitesi Veteriner Bilimleri Dergisi

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The objective of the present study was to evaluate clinical and haematological findings among 63 dogs solely diagnosed canine monocytic ehrlichiosis (CME) within IFAT application. For this purpose; age, breed, sex, anti-parasitic drug application, living environment, the presence of tick and general condition of the dogs were noticed, clinical and haematological variables were determined. Fourteen out of 63 (22.22%) dogs were regularly treated with anti-parasitic drugs while other 49 dogs (77.77%) were irregularly treated with anti-parasitic drugs. Forty-two (66.66%) of the dogs were living on garden conditions while 21 (33.33%) of the dogs were living in the house and 36 (57.14%) of the dogs had tick found on clinical referral. The most commonly observed clinical signs were anorexia (87.30%), lymphadenopathy (80.95%), pale mucous membranes (47.61%) and fever (42.85 %). Besides tachypnoea (36.50%), tachyarrhythmia (34.92%), haemorrhagia (22.22%), eye lesions (22.22%), skin lesions (7.93%), neurological signs (3.17%) and arthritis (19.04%) were other observed clinical signs. Thrombocytopenia (63.49%) and anaemia (46.03%) were most commonly detected haematological findings. In conclusion, it was suggested that CME was prevalent in our region and CME must be taken into consideration among dogs presenting aforementioned clinical and haematological findings. Öz: Bu çalışmanın amacı IFAT uygulaması ile sadece köpek monositik ehrlichiosis (KME) tanısı konulmuş 63 köpekteki klinik ve hematolojik bulguların değerlendirilmesidir. Bu amaçla; yaş, ırk, cinsiyet, anti-paraziter ilaç uygulamaları, yaşadığı ortam, kene varlığı ve genel durumla ilgili bilgiler kaydedilip, klinik ve hematolojik değişkenler belirlendi. Altmış üç köpekten 14'ünde (%22.2) anti-paraziter ilaç uygulamaları düzenliyken, 49 (%77.7) köpekte ise anti-paraziter ilaç uygulamaları düzensizdi. Köpeklerden 42'sinin (%66.6) bahçe ortamında, 21'inin (%33.3) ev ortamında yaşadığı ve 36 köpeğin (%57.1) kliniğe getirildiğinde üzerinde kene olduğu belirlendi. En sık gözlenen klinik bulgular; anoreksi (%87.30), lenfadenopati (%80.95), soluk mukoz membranlar (%47.61) ve ateş olarak tespit edilmiştir (%42.85). Ayrıca, Taşipne (%36.50), taşiaritmi (%34.92), hemoraji (%22.22), göz lezyonu (%22.22), deri lezyonu (%7.93), nörolojik bulgular (%3.17) ve arthritis (%19.04) gözlemlenen diğer klinik bulgular olarak belirlendi. Trombositopeni (%63.49) ve anemi (%46.03) en yaygın belirlenen hematolojik bulgular olarak dikkat çekmiştir. Sonuç olarak bölgemizde KME'in yaygın olarak görüldüğü ve yukarda belirtilen klinik ve hematolojik bulguların birlikte görüldüğü köpeklerde KME'inde dikkate alınması gerektiği kanısına varıldı.

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Hematobiochemical changes in ehrlichiosis in dogs of Anand region, Gujarat

Cited by 22 publications

References 7 publications

Canine Monocytic Ehrlichiosis among working dogs of organised kennels in India: A comprehensive analyses of clinico-pathology, serological and molecular epidemiological approach

Canine Monocytic Ehrlichiosis among working dogs of organised kennels in India: A comprehensive analyses of clinico-pathology, serological and molecular epidemiological approach

Interpretation of Coagulation Tendency Contributing to Thrombosis in Vector-Borne Diseases (Ehrlichiosis, Anaplasmosis, Leishmaniosis, and Dirofilariasis) among Dogs

Ege Bölgesindeki Köpeklerde Monositik Ehrlichiosis: Klinik ve Hematolojik Bulgular

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