Hematochemical Patterns in Follicular Fluid and Blood Stream in Cycling Mares: A Comparative Note

Satué, K.; Fazio, Esterina; Ferlazzo, Adriana; Medica, Pietro

doi:10.1016/j.jevs.2019.06.016

Cited by 8 publications

(5 citation statements)

References 41 publications

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“…Lipids may serve as the main substrate for aerobic energy production during oocyte maturation, as glucose is mostly directed for anaerobic energy production (Lewis et al 2020). Systemic and follicular triglyceride concentrations were consistent with a previous study from our group using the same methodology and follicle category (Sessions-Bresnahan et al 2016), with TG concentrations higher in plasma when compared to follicular fluid, although this relationship was not consistent with other equine studies (Collins et al 1997, Satué et al 2019. Triglyceride concentrations in follicular fluid were reduced after diet supplementation, although a similar decline was not noted in systemic concentrations.…”

Section: Discussionsupporting

confidence: 85%

Oocyte metabolic function, lipid composition, and developmental potential are altered by diet in older mares

et al. 2022

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Dietary supplementation is the most feasible method to improve oocyte function and developmental potential in vivo. During three experiments, oocytes were collected from maturing, dominant follicles of older mares to determine whether short-term dietary supplements can alter oocyte metabolic function, lipid composition, and developmental potential. Over approximately 8 weeks, control mares were fed hay (CON) or hay and grain products (COB). Treated mares received supplements designed for equine wellness and gastrointestinal health, flaxseed oil, and a proprietary blend of fatty acid and antioxidant support (reproductive support supplement (RSS)) intended to increase antioxidant activity and lipid oxidation. RSS was modified for individual experiments with additional antioxidants or altered concentrations of n-3 to n-6 fatty acids. Oocytes from mares supplemented with RSS when compared to COB had higher basal oxygen consumption, indicative of higher aerobic metabolism, and proportionately more aerobic to anaerobic metabolism. In the second experiment, oocytes collected from the same mares prior to (CON) and after approximately 8 weeks of RSS supplementation had significantly reduced oocyte lipid abundance. In the final experiment, COB was compared to RSS supplementation, including RSS modified to proportionately reduce n-3 fatty acids and increase n-6 fatty acids. The ability of sperm-injected oocytes to develop into blastocysts was higher for RSS, regardless of fatty acid content, than for COB. We demonstrated that short-term diet supplementation can directly affect oocyte function in older mares, resulting in oocytes with increased metabolic activity, reduced lipid content, and increased developmental potential.

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Section: Discussionsupporting

confidence: 85%

Oocyte metabolic function, lipid composition, and developmental potential are altered by diet in older mares

et al. 2022

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“…All year round, the maturation rate of equine oocytes was directly correlated with follicle size (Hinrichs and Schmidt, 2000). The follicular development in mares influences the follicular metabolites, electrolytes (Satué et al 2019) and steroid hormones (Satué et al 2020). Interestingly, FF components, related to the oocytes developmental competence, have been clearly described in cows (Annes et al 2019), camels (El-Shahat et al 2018, goats (Junior et al 2018), and sows (Bertoldo et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Lipid metabolites, interleukin-6 and oxidative stress markers in follicular fluid and their association with serum concentrations in mares

et al. 2023

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The application of trans-vaginal ovum pick up (OPU) and intracytoplasmic sperm injection (ICSI) is well established for commercial in vitro embryo production in horses. These assisted reproductive techniques are especially applied during the non-breeding season of the mare. However, little is known about how the health of the oocyte donor may affect the biochemical composition of the follicular fluid (FF) in small and medium-sized follicles routinely aspirated during OPU. This study aimed to investigate associations between systemic and FF concentrations of interleukin-6 (IL-6), total cholesterol, triglycerides, non-esterified fatty acids (NEFA), reactive oxygen metabolites (d-ROMs), biological antioxidant potential (BAP), and oxidative stress index (OSI) during the non-breeding season in mares. At the slaughterhouse, serum and FF of small (5-10 mm in diameter), medium (>10-20 mm in diameter), and large (>20-30 mm in diameter) follicles were sampled from 12 healthy mares.There was a strong positive association (P<0.01) between the concentration of IL-6 in serum and those measured in small (r= 0.846), medium (r= 0.999), and large (r= 0.996) follicles. Serum concentrations of NEFA were positively correlated (P<0.05) with those measured in small (r= 0.726), medium (r= 0.720), and large (r= 0.974) follicles. Values of total cholesterol and OSI in serum and medium follicles were significantly associated (r= 0.736 and r= 0.696, respectively). The serum concentrations of all lipid metabolites were markedly higher than those measured in FF of small-and medium-sized follicles. Values of IL-6 and OSI did not change significantly between serum and all follicle classes (P≥0.05). To conclude, changes in the blood composition associated with inflammation, oxidative stress, and disturbed lipid metabolism of mares may lead to an inadequate oocyte microenvironment, which could affect oocyte quality and the success rate of OPU/ICSI programs. Further research should indicate whether these changes may ultimately affect in vitro oocyte developmental capacity and subsequent embryo quality.

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“…The higher concentrations of AST and ALT in small follicles and medium follicles may also indicate the stage of early follicular atresia because when tissue is damaged, it leads to an increase in the concentration of transferases. [12] Alkaline phosphatase (ALP) is a lysosomal enzyme that catalyzes different reactions in the body, including the active transport of phosphates through the cell membrane, synthesis of proteins, and transfer of deoxyribonucleic acid to the nucleus. [13] The activity of ALP increases due to physiological phenomena, as well as pathological phenomena, such as inflammation and proliferation of cells.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Activity of enzymes in the follicular fluid and outcome of in vitro fertilization

Sorak,

Nikolov,

Sazdanovic

et al. 2024

Medicine

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Defining certain parameters of the oocyte microenvironment that can be simply and quickly detected and that enable differentiation of oocytes that have better or worse quality could potentially increase the efficacy of the in vitro fertilization (IVF) method. Follicular fluid represents the microenvironment for the development of oocytes during different stages of maturation. Enzymes present in the follicular fluid may affect the quality of oocytes. There are inconsistencies in the literature concerning enzyme concentration in follicular fluid. The main aim of this study was to examine whether the concentration of enzymes aspartate aminotransferase, alanine aminotransferase (ALT), alkaline phosphatase, and lactate dehydrogenase in follicular fluid can influence the outcome of IVF. The study included 120 patients who were involved in the process of IVF. We used the follicular fluid obtained after follicular aspiration in the ovaries as biological material for the analysis. The values of enzymes LDL (method—kinetic UV test), aspartate aminotransferase (method—kinetic UV test), ALT (method—kinetic UV test) and alkaline phosphatase (method—colorimetric kinetic test) in the follicular fluid were determined on the analyzer АU 680, Becman Coulter. The criteria of the Istanbul consensus of clinical embryologists were used for the embryo assessment. The software package SPSS 20 was used for the statistical processing of data. The results of follicular fluid sample analysis showed a correlation between ALT concentration in the follicular fluid and IVF outcome. Based on our results, we can conclude that ALT concentration affects the outcome of IVF.

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Hematochemical Patterns in Follicular Fluid and Blood Stream in Cycling Mares: A Comparative Note

Cited by 8 publications

References 41 publications

Oocyte metabolic function, lipid composition, and developmental potential are altered by diet in older mares

Oocyte metabolic function, lipid composition, and developmental potential are altered by diet in older mares

Lipid metabolites, interleukin-6 and oxidative stress markers in follicular fluid and their association with serum concentrations in mares

Activity of enzymes in the follicular fluid and outcome of in vitro fertilization

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