Hematologic Techniques for Quantitative Assessment in Reptiles and Amphibians

doi:10.1002/9781119660293.ch18

Cited by 3 publications

(4 citation statements)

References 28 publications

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“…13,27 Bas vary from species to species, being quite frequent in reptiles and suffering influence from habitat. 12,27 In addition, basophilia has been associated with parasitic and viral infections. 13,59 No clinical changes were observed in captive individuals, but environmental variations in exposure to disease, stress, and other immune-stimulating factors should be considered a plausible explanation for differences in Bas measures.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of Season, Environment, and Sex on Selected Blood Parameters of Geoffroy's Side-Necked Turtles, Phrynops Geoffroanus (Schweigger, 1812)

Pulcherio,

Rocha,

de Barros

et al. 2023

Journal of Zoo and Wildlife Medicine

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Section: Discussionmentioning

confidence: 99%

“…The TS was performed by clinical refractometer (Instrutemp, Belenzinho, São Paulo, São Paulo, Brazil, 03059-023) using plasma from the microhematocrit tube. 12…”

Section: Methodsmentioning

confidence: 99%

Influence of Season, Environment, and Sex on Selected Blood Parameters of Geoffroy's Side-Necked Turtles, Phrynops Geoffroanus (Schweigger, 1812)

Pulcherio,

Rocha,

de Barros

et al. 2023

Journal of Zoo and Wildlife Medicine

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“…A PCV was determined with a microhematocrit centrifuge (Pico 17 centrifuge, Haraeus, Thermo Fisher Scientific), and TP of plasma was measured using a digital refractometer (Palm Abbe Digital Refractometer, Misco, Cleveland, OH). A WBC count was obtained using a Neubauer hemocytometer and Phloxine B solution ( 10 ). The phloxine B solution was made in-house from powder (Sigma-Aldrich, St Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

“…The total WBC count was based only on the heterophil percentage due to the light basophilic appearance of the eosinophils and their unknown uptake with the eosin stain. The equation used to determine the total calculated WBC count in this specific case was [(total count of eosin-stained WBCs on both sides of hemocytometer) x 1.1 × 16 × 100] / % heterophils from the differential ( 10 ). A 200 WBC differential count was performed on a blood film stained with modified Wright’s stain (Hema-tek 1,000, Siemens Healthcare Diagnostics Inc., Tarrytown, NJ) by one reviewer (TS); the calculated WBC count was concordant with the WBC estimate, as described above, from the blood film.…”

Section: Methodsmentioning

confidence: 99%

A mystery revealed: an update on eosinophil and other blood cell morphology of the Argentine black and white tegu (Salvator merianae)

Bosch,

Stacy,

Armien

et al. 2024

Front. Vet. Sci.

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Reptile white blood cell (WBC) morphological features are strikingly variable across species. In the Argentine black and white tegu (Salvator merianae), red tegu (Salvator rufescens), and Savannah monitor (Varanus exanthematicus), previous reports described a WBC type with a single distinct, clear, linear- to ovoid- to crescent-shaped inclusion of presumptive monocytic origin. The objective of this study was to further investigate the origin of this unique WBC type with crescent-shaped inclusions. Blood samples from two Argentine black and white tegus, tegu 1, a 4-year-old female, and tegu 2, a 2-year-old presumed male, were submitted for routine hematological evaluation. Additional blood films were prepared and stained with these cytochemical stains: alkaline phosphatase (ALP; naphthol AS-MX phosphate substrate), alpha-naphthyl butyrate esterase, alpha-chloroacetate esterase, myeloperoxidase, Periodic acid-Schiff, and Sudan black B. Blood films from tegu 1 were also stained with a second ALP stain (5-bromo-4-chloro-3-indoxyl-phosphate and nitroblue tetrazolium substrate), Luna, luxol fast blue, and toluidine blue. The blood from tegu 1 was cytocentrifuged to isolate and fix the buffy coat in glutaraldehyde 2.5% aqueous solution for transmission electron microscopy. Six morphologically distinct WBC types were identified from tegu 1, including heterophils, basophils, monocytes, azurophils, lymphocytes, and the unique WBC type, which were identified as eosinophils with inclusions. WBC types in tegu 2 were similar; however, eosinophils lacked a discernable inclusion. Proper WBC identification will be useful in obtaining accurate hemogram data for this species.

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Location has a significant effect on body condition and blood parameters in the eastern longneck turtle (Chelodina longicollis)

Scheelings

2024

Aust. J. Zool.

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The aim of this investigation was to explore the effect point of capture has on relative weight (Wr), as well as haematology and biochemistry values, in wild eastern longneck turtles (Chelodina longicollis). This study group consisted of two sites of turtles residing in Duck Pond (DP) and Ivanhoe Wetland (IW) in the Darebin Parklands, in Alphington, Melbourne. From DP, 184 turtles were captured, and from IW, 37 turtles were captured. All turtles were weighed and measured, and a random subset of 20 turtles from each waterbody was selected for blood collection. Significant differences were found to exist for Wr, basophils, glucose, uric acid, triglycerides and bile acids between the two sites. Serum glucose levels tended to decrease as a turtle increased in mass, straight carapace length (SCL) and Wr. The results of this investigation highlight the need to take location into consideration when assessing blood parameters in reptiles.

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Hematologic Techniques for Quantitative Assessment in Reptiles and Amphibians

Cited by 3 publications

References 28 publications

Influence of Season, Environment, and Sex on Selected Blood Parameters of Geoffroy's Side-Necked Turtles, Phrynops Geoffroanus (Schweigger, 1812)

Influence of Season, Environment, and Sex on Selected Blood Parameters of Geoffroy's Side-Necked Turtles, Phrynops Geoffroanus (Schweigger, 1812)

A mystery revealed: an update on eosinophil and other blood cell morphology of the Argentine black and white tegu (Salvator merianae)

Location has a significant effect on body condition and blood parameters in the eastern longneck turtle (Chelodina longicollis)

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