Hematological, biochemical, and blood gas alterations of goat whole blood stored in CPDA-1 and CPD/SAG-M plastic bags

Abstract

The aim of this study was to evaluate the hematological, biochemical, and blood gas alterations of goat whole blood stored in different blood bags. Seven male, adult, crossbreed goats were used, weighing 62±1.8 kg. Nine hundred milliliters of whole blood from each animal was collected and stored in blood bags (450 ml in each), CPDA-1 (citrate phosphate dextrose-adenine) and CPD/SAG-M (citrate phosphate dextrose with saline-glucose-mannitol) as additive solutions, and kept refrigerated (2-4 ºC) for 42 days. Blood samples were collected from the plastic bags at baseline (T0) and after seven, 14, 21,28, 35, and 42 days for hematological, biochemical, blood gas, and microbiological evaluations. Free hemoglobin, degree of hemolysis, lactate, and pO2were increased in both bags, whereas hydrogen potential (pH) and the total hemoglobin concentration decreased overtime(P<0.05). The red blood cell count, glucose, sodium, and potassium remained stable, compared to the baseline. The CPD/SAG-M bag presented a lower red cell count, globular volume, total hemoglobin, and sodium, and a higher degree of hemolysis and plasma hemoglobin, compared with the CPDA-1 bag. The whole goat blood remained viable for therapeutic use; although, there were some important changes in the variables of the 42-day stored blood in relation to fresh blood (T0). We concluded that the CPDA-1 bag is more suitable for use in the storage of goat blood because of its lower commercial value.

Effect of storage on biochemical parameters of packed red blood cells of goats in............ 257-261 citrate phosphate dextrose adenine/ saline adenine glucose mannitol

“…The mean value of glucose in supernatant reduced as the storage period increased. This is in agreement with Tavares (2013), who observed decrease in glucose values during storage from day 0 to 42. This reduction might be due to the consumption of glucose by the metabolically active erythrocytes as an energy source.…”

Assessment of Donkey (Equus asinus africanus) Whole Blood Stored in CPDA-1 and CPD/SAG-M Blood Bags

“…The SAG/M blood bag was developed for human blood. In our study, as in other studies with animal species, this bag did not result in an additional improvement of stored blood quality [ 9 ]. This may be related to the specificity of animal blood, and animal-designed blood bags may be required to improve blood parameters.…”

“…The CPDA-1 bags contain a solution of citrate, phosphate, dextrose, and adenine (63 mL) that allows the storage of human blood for 35 days, while the CPD/SAG-M bags, in addition to citrate, phosphate, and dextrose (63 mL) have an additive solution in a satellite bag containing saline, adenine, and mannitol (100 mL) that provides a greater supply of energy and phosphate for the survival of red blood cells. CPDA-1 bags are cheaper and easier to find than CPD/SAG-M [ 9 ].…”

“…Blood bags containing citrate-phosphate-dextrose and adenine (CPDA-1) are the most commonly used ones to preserve blood under refrigeration in veterinary medicine [ 10 ]. CPDA-1-type blood bags have better red blood cell preservation properties than ACD (acid citrate dextrose), allowing whole blood storage for canines, [ 11 ] equines, [ 5 , 12 ] bovines, [ 13 ] sheep, [ 10 ] goats, [ 9 ] and buffaloes [ 14 ] for up to 35 days. In small animal transfusion medicine, a reduction in storage time has been observed, with the maximum storage time varying between 21 and 28 days [ 15 , 16 , 17 ].…”

The Effects of Pre-Storage Leukoreduction on the Conservation of Bovine Whole Blood in Plastic Bags

“…Although blood fractionation techniques are used to obtain individual blood components, the conservation and use of whole blood is still the most used method [16][17][18] in large animal clinics where it is more difficult to use fractionated blood. At the field level, it is necessary to invest in more infrastructure and equipment to perform fractionation.…”