Hematological dynamics following the co-administration of Resveratrol and Cisplatin in Sprague–Dawley rats.

Abstract

Background: Cisplatin, a platinum-based chemotherapy agent, is used in the effective treatment of a wide range of malignant cancers. Resveratrol, a polyphenolic plant-derived compound, has been shown to have several biological effects including protecting the body against several forms of damages.Objective: This study assessed the effects of cisplatin and supplementation with resveratrol on some hematological parameters in Sprague-Dawley rats.Material and Methods: Forty-five adult female Sprague-Dawley rats, grouped into 9, were used for this experimental study. Group 1 served as the control group and received distilled water only. Groups 2 and 9 received Cisplatin only while groups 3, 4, and 5 received different doses of Resveratrol after a single dose of Cisplatin. Groups 6, 7, and 8 received Resveratrol before Cisplatin.Results: At the end of administration, blood samples were collected and different hematological parameters were accessed. Groups 2 and 5 showed a significantly higher Total WBC when compared to the control group (p<0.05). Group 7 showed a significantly higher (p<0.05) neutrophil count compared to both the control group and group 2, but a significant decrease in the lymphocyte count compared to the same groups (p<0.05). Groups 6 and 7 showed a significant reduction in monocyte when compared to the control group (p<0.05). There was no significant difference in the eosinophil count of all treatment groups when compared to the control group and cisplatin groups (p<0.05). Basophil increased significantly in group 7 when compared to the control group and group 2 (p<0.05). More so, a significant decrease in the Haemoglobin (Hb), Packed cell volume (PCV) and Mean corpuscular volume (MCV) level were observed when compared to group 9 (p<0.05).Conclusion: This study showed that cisplatin-induced alteration in some hematological parameters is reversible by treatment with resveratrol. More so, resveratrol supplementation should be incorporated as part of dietary nutrients for patients on cisplatin during chemotherapy. ТҰЖЫРЫМДАМА Кіріспе: Цисплатин -бұл түрлі қатерлі ісіктерді тиімді емдеуде пайдаланылатын химиотерапевтикалық препараты құрайтын пла-тин. Ресвератрол, шығуы өсімдік түрінен болатын полифенольді байланысудың зақымдалудың кейбір формаларынан организмді қорғауды қосқанда, бірнеше биологиялық әсері бар екендігі дәлелденді.Мақсаты: Осы зерттеуде Спрег-Доули егеуқұйрықтарындағы кейбір гематологиялық параметрлерге ресвератролдың қоспалары және цисплатиннің әсері бағаланды.Материалдар мен әдістер: Осы зерттеуде 9 топқа бөлінген қырық бес ересек Спрег-Доули егеуқұйрықтары қолданылды. 1-топ бақылау тобы болды және дистилденген суды ғана қабылдады. 2-ші және 9-шы топтар тек қана цисплатин қабылдады, сол уақытта 3, 4-ші Цель: В данном исследовании оценивались эффекты цисплатина и добавки ресвератрола на некоторые гематологические параметры у крыс Спрег-Доули.Материалы и методы: В данном исследовании было использовано сорок пять взрослых крыс Спрег-Доули, разделенных на 9 групп. Группа 1 стала кон...

Evaluation of the therapeutic efficacy of Vitex agnus-castus extract on cisplatin-induced hematotoxicity in female Wistar rats

“…We also observed that the total WBC count returned to normal (i.e., pre-CP levels) around day 6 (144 h) after CP treatment, which aligns with the previous study by Nowrousian and Schmidt [ 30 ]. This increase in the WBCs on day 6 might be due to infection and inflammation during CP administration and CP metabolism in the rats [ 9 , 31 ]. While similar effects were observed in both VAC-treated groups, the extent of decrease in the total WBCs on day 3 in the VAC-350 group was lesser than that in the VAC-650 group, indicating that treatment with 350 mg/kg b.w.…”