Hematological parameters and nuclear abnormalities in peripheral erythrocytes of Achirus lineatus (Pleuronectiformes: Achiridae)

Abstract

Hematological parameter of demersal fish constitutes important measures of biological effects of seasonality and contaminants in sediments. We aimed to assess the hematological parameters and nuclear abnormalities, including micronuclei levels, in peripheral erythrocytes of flatfish Achirus lineatus collected in São Vicente Estuary in summer and winter. The number of lymphocytes was significantly higher in summer, whereas the number of neutrophils was significantly higher in winter. For other measured parameters, no significant differences were observed in spite of the levels of erythroblasts, leukocytes, thrombocytes, micronuclei and nuclear abnormality tended to be slightly higher in winter. Integrated analysis of data suggests that genotoxicity may be related to immunosuppression, although some types of leukocytes appear to act removing anomalous cells. Considering the contamination status of the Santos-São Vicente Estuarine system, the results provide an important contribution to knowledge of the hematological aspects of A.lineatus and its potential use as a bioindicator for monitoring estuarine sites.

Yucca schidigeraextract mediated the growth performance, hepato‐renal function, antioxidative status and histopathological alterations in Nile tilapia (Oreochromis niloticus) exposed to hypoxia stress

“…During stress, secretion of cortisol curtails life span of lymphocytes promoting their apoptosis (Verburg van Kemenade et al., 1999; Wyets et al, 1998) that causes alterations in cell number. Under stress conditions, most often circulating lymphocytes decrease in number perhaps because of distribution between organs and also owing to lymphopoietic activity (Prado et al., 2015).…”

Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond

“…Micronuclei, which were defined as two completely separated nuclei within a single erythrocyte's cytoplasm, were identified, and their frequencies were recorded. Nuclear abnormalities are understood as precursors of further DNA damage, and they include invaginations of the nuclear envelope of different sizes, noticeable depressions in the center in which nuclear material was otherwise absent, as well as other morphological abnormalities that did not fit into the previous categories (Bucker et al 2012;Prado et al 2014;Seriani et al 2015b;Thomé et al 2016).…”

“…In urban areas, aquatic ecosystems are more susceptible to degradation, and many vertebrates and invertebrates have been used to monitor environmental quality. Changes in functional enzymes (Bonnail et al 2016;Vieira et al 2017;Sadauskas-Henrique et al 2017), effects of cytogenotoxicity (Prado, et al 2014, de Almeida Duarte et al 2016, mutagenicity (Araújo al. 2014;Weldetinsae et al 2017), and hematological, immunological, and histopathological changes (da Cruz et al, 2015;Corrêa et al 2017;Thummabancha et al 2016;Hedayati and Darabitabar 2017) all represent well-documented biomarkers used in environmental monitoring.…”

Differential blood counting in fish as a non-destructive biomarker of water contamination exposure

“…The characteristics of fish blood have long been used for assessing the physiological status of fish exposed to environmental stressors (França et al 2007;Prado et al 2014). Because blood flows continuously through the body and perfuses all organs and tissues, it integrates several levels of biological organization (physiology, histology, cytology, and hormonal regulation); thus, hematology may provide reliable information on the health status of a specimen.…”