Hematological parameters and selected intestinal microbiota populations in the Indonesian indigenous crossbred chickens fed basal diet supplemented with multi-strain probiotic preparation in combination with vitamins and minerals

Abstract

Aim:The aim of this study was to investigate the effect of dietary supplementation with multi-strain probiotic preparation in combination with vitamins and minerals on the hematological parameters and selected intestinal microbiota populations in the Indonesian indigenous crossbred chickens.Materials and Methods:A total of 240 one-day-old Indonesian indigenous crossbred chicks were raised for 10 weeks. The chicks were distributed to one of four groups, i.e., chicks receiving basal diet without any additive (CONT), chicks receiving basal diet with 0.04% of zinc bacitracin (AGP), chicks receiving basal diet with 0.01% of commercial probiotic Bacillus subtilis preparation (PROB1), and chicks receiving basal diet with 0.5% of multi-strain probiotic preparation in combination with vitamins and minerals (PROB2). Blood was collected on the week 8, while the internal organs and eviscerated carcasses were collected on the week 10.Results:PROB2 tended (p=0.09) to have a lower body weight (BW) compared to CONT chicks. Feed conversion ratio was higher (p<0.05) in PROB1 and PROB2 compared to CONT birds. The number of thrombocytes tended (p=0.09) to be higher in CONT than in other groups. Antibody titer against Newcastle disease virus vaccine was higher (p<0.05) in PROB1 and PROB2 than in CONT group. Serum triglyceride concentration was lower (p<0.05) in PROB2 than in other birds. AGP chicks had lower (p<0.05) serum total protein and globulin concentrations than CONT and PROB1 chicks. Serum albumin level was lower (p<0.05) in PROB2 than in CONT and PROB1 birds. Albumin to globulin ratio tended (p=0.06) to be higher in AGP than in other birds. Lactose-negative Enterobacteriaceae tended (p=0.07) to be lower in PROB1 and PROB2 than in CONT group. PROB1 and PROB2 tended (p=0.06) to have greater lactic acid bacteria (LAB) population than in CONT and AGP birds.Conclusion:Multi-strain probiotic preparation in combination with vitamins and minerals was able to improve immune response and control the potentially pathogenic bacteria. However, the additive could not improve the growth performance of the Indonesian indigenous crossbred chickens.

Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers

“…Probiotic supplementation with heat stress conditions significantly improved the hematological measurements in broiler chicks ( 59 ). However, Sugiharto et al ( 60 ) reported that probiotics have no effect on the chicks' hematology in normal environmental conditions. On the other side, the results of the leukogram were negatively affected under thermal stress.…”

The Benefit of Hedgerow Access on the Health and Growth Rate of Pasture Raised Broiler Chickens

“…Whatever benefit was conferred by the access to the hedgerow appeared to be preferentially allocated to production performance in the Cornish cross and immune function in the Red rangers. This is makes sense when considering the intended use for each genotype; fast growing Cornish cross were developed to achieve maximum growth rates in the sterile, tightly regulated environment of conventional indoor poultry-houses (Aviagen, 2018), whereas slower growing Red rangers retain the ancestral ability to allocate resources into the development of energetically expensive adaptive mechanisms such as hemodynamic cooling and immune surveillance (Donkoh, 1989;Wideman et al, 1994;Sugiharto et al, 2018). These findings are consistent with a phenomenon that has been well-documented in broiler chickens, in which there exists a genetic trade-off between maximum production performance and the ability to adapt to environmental challenges (Cahaner and Leenstra, 1992;Deeb and Cahaner, 2002;Ask et al, 2006;Azad et al, 2010;Castellini et al, 2016;Duangjinda et al, 2017).…”

The growth performance of Peking ducks fed with rations containing probiotic agents and waste products of leubiem fish (Chanthidermis maculatus)

“…Similarly, feed additives such as probiotics are needed to improve the productivity and performance of Peking ducks. Probiotics are a group of beneficial microbes used to influence the host through the repair of the microorganisms in the digestible tract [4]. The most commonly used probiotic microorganisms are the Lactobacillus and the Bifidobacteria strains [5].…”