Hematopoietic peripheral circulating blood stem cells as an independent marker of good transfusion management in patients with β‐thalassemia: results from a preliminary study

Napolitano, Mariasanta; Gerardi, Calogera; Lucia, Anna Di; Accardo, P; Rizzuto, Lucia; Ferraro, Maria; Siragusa, Sergio; Buscemi, Filippo

doi:10.1111/trf.13452

Cited by 6 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In mice, most steady-state circulating PB HSPCs show no long-term repopulating potential 25 , often fail to re-enter the BM 18,26,27 , or change phenotype after their egress 27 . In humans, increased frequencies of CD34 + cells in PB are observed in many hematopoietic diseases, notably in sickle cell anemia and b-thalassemia, and in cardiovascular, autoimmune and rheumatological conditions 21,[28][29][30][31][32][33][34] , but with very few studies providing further resolution of CD34 + subsets. It has long been assumed that steady-state PB HSPC composition mirrors that of the BM but no baseline steady-state circulating HSPC composition has been reported to date.…”

Section: Introductionmentioning

confidence: 99%

Unique molecular and functional features of extramedullary hematopoietic stem and progenitor cell reservoirs in humans

Mende¹,

Bastos

Santoro

et al. 2022

Blood

View full text Add to dashboard Cite

Rare hematopoietic stem and progenitor cell (HSPC) pools outside the bone marrow (BM) contribute to blood production in stress and disease but remain ill-defined. Although non-mobilized peripheral blood (PB) is routinely sampled for clinical management, the diagnosis and monitoring potential of PB HSPCs remains untapped, as no healthy PB HSPC baseline has been reported. Here we comprehensively delineate human extramedullary HSPC compartments comparing spleen, PB and mobilized PB (mPB) to BM using single-cell RNA-seq and/or functional assays. We uncover HSPC features shared by extramedullary tissues and others unique to PB. First, in contrast to actively dividing BM HSPCs, we find no evidence of substantial ongoing hematopoiesis in extramedullary tissues at steady state, but report increased splenic HSPC proliferative output during stress erythropoiesis. Second, extramedullary stem cells/multipotent progenitors (HSC/MPPs) from spleen, PB and mPB share a common transcriptional signature and increased abundance of lineage-primed subsets compared to BM. Third, healthy PB HSPCs display a unique bias towards erythroid-megakaryocytic differentiation. At HSC/MPP level, this is functionally imparted by a subset of phenotypic CD71+ HSC/MPPs, exclusively producing erythrocytes and megakaryocytes, highly abundant in PB but rare in other adult tissues. Finally, the unique erythroid-megakaryocytic-skewing of PB is perturbed with age, in essential thrombocythemia and in beta-thalassemia. Collectively, we identify extramedullary lineage-primed HSPC reservoirs that are non-proliferative in situ and report involvement of splenic HSPCs during demand-adapted hematopoiesis. Our data also establish aberrant composition and function of circulating HSPCs as potential clinical indicators of BM dysfunction.

show abstract

Section: Introductionmentioning

confidence: 99%

Unique molecular and functional features of extramedullary hematopoietic stem and progenitor cell reservoirs in humans

Mende¹,

Bastos

Santoro

et al. 2022

Blood

View full text Add to dashboard Cite

show abstract

“…Various agents are capable of mobilizing the HSPC from the BM niche to the PB, increasing the accessibility and the number of cells available for transplantation (38,39). Under physiological conditions, few HSPC are found in PB of untreated subjects (40)(41)(42) and, in absence of pharmacological mobilization, their amount in the periphery is a marker of disease progression and/or of treatment efficacy for several hematopoietic disorders (43)(44)(45)(46).…”

mentioning

confidence: 99%

Multiparametric Whole Blood Dissection: A one‐shot comprehensive picture of the human hematopoietic system

et al. 2017

View full text Add to dashboard Cite

Human hematopoiesis is a complex and dynamic system where morphologically and functionally diverse mature cell types are generated and maintained throughout life by bone marrow (BM) Hematopoietic Stem/Progenitor Cells (HSPC). Congenital and acquired hematopoietic disorders are often diagnosed through the detection of aberrant frequency or composition of hematopoietic cell populations. We here describe a novel protocol, called “Whole Blood Dissection” (WBD), capable of analyzing in a single test‐tube, hematopoietic progenitors and all major mature cell lineages composing either BM or peripheral blood (PB) through a multiparametric flow‐cytometry analysis. WBD allows unambiguously identifying in the same tube up to 23 different blood cell types including HSPC subtypes and all the major myeloid and lymphoid lineage compartments at different stages of maturation, through a combination of 17 surface and 1 viability cell markers. We assessed the efficacy of WBD by analyzing BM and PB samples from adult (n = 8) and pediatric (n = 9) healthy donors highlighting age‐related shift in cell composition. We also tested the capability of WBD on detecting aberrant hematopoietic cell composition in clinical samples of patients with primary immunodeficiency or leukemia unveiling expected and novel hematopoietic unbalances. Overall, WBD allows unambiguously identifying >99% of the cell subpopulations composing a blood sample in a reproducible, standardized, cost‐, and time‐efficient manner. This tool has a wide range of potential pre‐clinical and clinical applications going from the characterization of hematopoietic disorders to the monitoring of hematopoietic reconstitution in patients after transplant or gene therapy. © 2017 The Authors. Cytometry Part A Published by Wiley Periodicals, Inc. on behalf of ISAC.

show abstract

“…Em alguns casos, como durante a gravidez, a suplementação de ácido fólico pode trazer benefícios para pessoas com essa condição (AL-NOOD, 2009). A talassemia intermediária pode exigir instruções de transfusão de sangue para aumentar o suprimento de glóbulos vermelhos (NAPOLITANO et al, 2016;YESILIPEK, 2020). Pessoas com talassemia major necessitam transfusões de sangue regulares e medicamentos para remover o excesso de ferro que se acumulou em certos órgãos (terapia de quelação de ferro).…”

Section: Discussõesunclassified

“…Os pacientes sempre buscavam métodos populares de tratamento, o que muitas vezes prejudicava o processo curativo. Alguns desses métodos eram: cozinhar em panela de ferro, ingerir maior quantidade de legumes ricos em ferro, mostrando uma associação entre conhecimento leigo e médico (BOU-FAKHREDIN et al, 2021;NAPOLITANO et al, 2016).…”

Section: Discussõesunclassified

Anemias e suas correlações clínicas

Marques¹,

Figueredo²

2022

View full text Add to dashboard Cite

Hematopoietic peripheral circulating blood stem cells as an independent marker of good transfusion management in patients with β‐thalassemia: results from a preliminary study

Abstract: Circulating peripheral CD34 + stem cells are increased in β-thalassemia, in particular in nontransfused patients, compared to healthy controls.

Cited by 6 publications

References 13 publications

Unique molecular and functional features of extramedullary hematopoietic stem and progenitor cell reservoirs in humans

Unique molecular and functional features of extramedullary hematopoietic stem and progenitor cell reservoirs in humans

Multiparametric Whole Blood Dissection: A one‐shot comprehensive picture of the human hematopoietic system

Anemias e suas correlações clínicas

Contact Info

Product

Resources

About