Hematopoietic stem cells: from the bone to the bioreactor

Cabrita, Gonçalo J. M.; Ferreira, Bruno Sommer; Silva, Cláudia Lobato da; Gonçalves, Raquel; Almeida-Porada, Graça; Cabral, Joaquim M. S.

doi:10.1016/s0167-7799(03)00076-3

Cited by 119 publications

(92 citation statements)

References 63 publications

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“…The ability to generate large numbers of HPS cell-derived differentiated cells is critical to developing these strategies. Stirred suspension cultures are a popular approach to scale-up static dish-based cell cultures because they improve culture homogeneity, permit online monitoring and control of pH and dissolved oxygen concentration, and continuous medium perfusion can be easily enabled (Collins, Miller et al 1998;Madlambayan, Rogers et al 2001;Cabrita, Ferreira et al 2003;Kwon, Kim et al 2003;Zandstra, Bauwens et al 2003;Dang, Gerecht-Nir et al 2004;Bauwens, Yin et al 2005). …”

Section: Scalable Production Of Hpsc-derived Cardiomyocytesmentioning

confidence: 99%

Geometric Control of Cardiomyogenic Induction in Human Pluripotent Stem Cells

Bauwens

Song

Thavandiran

et al. 2011

Tissue Engineering Part A

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Pluripotent stem cells provide the opportunity to study human cardiogenesis in vitro, and are a renewable source of tissue for drug testing and disease models, including replacement cardiomyocytes that may be a useful treatment for heart failure. Typically, differentiation is initiated by forming spherical cell aggregates wherein an extraembryonic endoderm (ExE) layer develops on the surface. Given that interactions between endoderm and mesoderm influence embryonic cardiogenesis, we examined the impact of human embryonic stem cell (hESC) aggregate size on endoderm and cardiac development. We first demonstrated aggregate size control by micropatterning hESC colonies at defined diameters and transferring the colonies to suspension. The ratio of endoderm (GATA-6) to neural (PAX6) gene and protein expression increased with decreasing colony size. Subsequently, maximum mesoderm and cardiac induction occurred in larger aggregates when initiated with endoderm-biased hESCs (high GATA-6:PAX6), and in smaller aggregates when initiated with neural-biased hESCs (low GATA-6:PAX6). Additionally, incorporating micropatterned aggregates in a stirred suspension bioreactor increased cell yields and contracting aggregate frequency. We next interrogated the relationship between aggregate size and endoderm and cardiac differentiation efficiency in sizecontrolled aggregates, generated using forced aggregation, in defined cardiogenic medium.

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Section: Scalable Production Of Hpsc-derived Cardiomyocytesmentioning

confidence: 99%

Geometric Control of Cardiomyogenic Induction in Human Pluripotent Stem Cells

Bauwens

Song

Thavandiran

et al. 2011

Tissue Engineering Part A

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“…Tissue engineering has progressed in areas relevant to the developments of novel biomaterials [17], the designs of bioreactors for dynamic in vitro culture systems [18], and the uses of diverse cell sources including appropriate multi-potent stem cells [19]. For the construction of soft tissues or even entire organs, however, huge technical challenges remain to be overcome.…”

Section: Resultsmentioning

confidence: 99%

Layer-by-layer printing of cells and its application to tissue engineering

Kesari¹,

Xu²,

Boland³

2004

MRS Proc.

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Tissues and organs exhibit distinct shapes and functions nurtured by vascular connectivity.In order to mimic and examine these intricate structure-function relationships, it is necessary to develop efficient strategies for assembling tissue-like constructs.Many of the top-down fabrication techniques used to build microelectromechanical systems, including photolithography, are attractive due to the similar feature sizes, but are not suitable for delicate biological systems or aqueous environments. A layer-by layer approach has been proposed by us to pattern functional cell structures in three dimensions. Freeform cell structures are created by the inkjet method, in which cells are entrapped within hydrogels and crosslinked on demand. The cells are viable, functional and show potential for cell maturation as exemplified by the diversion of hematopoietic stem cells into multiple cell types. These results show promise for many tissue engineering applications.

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“…Several kinds of bioreactors have been applied in the field of HSCs expansion, including stirred tank bioreactor, fixed bed bioreactor and perfusion chamber [73][74][75]. It is known that hematopoietic cells are extremely sensitive to shear force, hence cells may suffer some physical damage under shear environment like in a stirred tank bioreactor and perfusion chambers [73].…”

Section: Ex Vivo Expansion Of Hscmentioning

confidence: 99%

Hematopoietic stem cells from peripheral blood the perspective of non-mobilized peripheral blood

Katsares¹,

Paparidis²,

Nikolaidou³

et al. 2010

Health

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The peripheral blood is a major source of hematopoietic stem cells. Almost for two decades the peripheral blood has been mobilized, in order to enhance the CD34 + concentration. The isolated stem cells from the mobilized peripheral blood are used as an alternative, or in addition to bone marrow derived stem cells. In this paper, a new perspective is being discussed; the use of non-mobilized peripheral blood as an alternative source for hematopoietic progenitor cells. The number of isolated hematopoietic stem cells is evaluated using flow cytometry. The viability can be evaluated using the trypan blue exclusion test, the flow cytometry or automated assays. The isolated hematopoietic stem cells could be used for ex vivo expansion either in static systems or in proper bioreactor systems, prior to cryopreservation and/or transplantation.

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Hematopoietic stem cells: from the bone to the bioreactor

Cited by 119 publications

References 63 publications

Geometric Control of Cardiomyogenic Induction in Human Pluripotent Stem Cells

Geometric Control of Cardiomyogenic Induction in Human Pluripotent Stem Cells

Layer-by-layer printing of cells and its application to tissue engineering

Hematopoietic stem cells from peripheral blood the perspective of non-mobilized peripheral blood

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