Heme oxygenase 1 plays a crucial role in swamp eel response to oxidative stress induced by cadmium exposure or Aeromonas hydrophila infection

Zang, Yuwei; Zheng, Shuting; Tang, Fang; Yang, Long; Wei, Xiping; Kong, Dan; Sun, Wenxiu; Li, Wei

doi:10.1007/s10695-020-00846-0

Cited by 13 publications

(6 citation statements)

References 57 publications

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“…34,35 In MRSA, genes (isdI and SAOUHSC01282) expressing cytoprotective enzymes like glutathione peroxidases against oxidation were also up-regulated. 36,37 On the other hand, the ROS-induced DNA damage effect activated the DNA repair machinery in the bacteria. For instance, rpo family genes (rpoE, rpoY and rpoZ) with DNA repair functions were all upregulated in PdH treated CRSA and MRSA.…”

Section: Genome Analysismentioning

confidence: 99%

Revealing the antibacterial power of hydrogen-releasing PdH nanohydride against drug resistant Staphylococcus aureus: an in-depth mechanism study

Song

Liu

et al. 2023

J. Mater. Chem. B

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Section: Genome Analysismentioning

confidence: 99%

Revealing the antibacterial power of hydrogen-releasing PdH nanohydride against drug resistant Staphylococcus aureus: an in-depth mechanism study

Song

Liu

et al. 2023

J. Mater. Chem. B

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“…Moderate dose ALR alleviates oxidative damage, but a high dose counteracts this positive effect (Yang et al ., 2022). Stress is known to increase expression levels of ALR, HO1 and Nrf2 (Jung et al ., 2013; Yang et al ., 2022; Zang et al ., 2020). This study showed that bacterial infection greatly increased the expression level of Alr at the late stage and significantly decreased the expression of Nrf2.…”

Section: Discussionmentioning

confidence: 99%

“…A. hydrophila challenge was performed as described by Zang et al . (2020). Bacteria were cultured at 28°C until the mid‐logarithmic growth phase and then diluted in normal saline (0.9%) to a concentration of 10 6 CFU ml −1 .…”

Section: Methodsmentioning

confidence: 99%

Swamp eel aldehyde reductase is involved in response to nitrosative stress via regulating NO/GSH levels

Zhao

Yang

et al. 2023

Journal of Fish Biology

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Aldehyde reductase (ALR) plays key roles in the detoxification of toxic aldehyde. In this study, the authors cloned the swamp eel ALR gene using rapid amplification of cDNA ends‐PCR (RACE‐PCR). The recombinant protein (rALR) was expressed in Escherichia coli and purified using a Ni2+‐NTA chelating column. The rALR protein exhibited efficient reductive activity towards several aldehydes, ketones and S‐nitrosoglutathione (GSNO). A spot assay suggested that the recombinant E. coli strain expressing rALR showed better resistance to formaldehyde, sodium nitrite and GSNO stress, suggesting that swamp eel ALR is crucial for redox homeostasis in vivo. Consequently, the authors investigated the effect of rALR on the oxidative parameters of the liver in swamp eels challenged with Aeromonas hydrophila. The hepatic glutathione (GSH) content significantly increased, and the hepatic NO content and levels of reactive oxygen species and reactive nitrogen species significantly decreased when rALR was administered. In addition, the mRNA expression of hepatic Alr, HO1 and Nrf2 was significantly upregulated, whereas the expression levels of NF‐κB, IL‐1β and NOS1 were significantly downregulated in the rALR‐administered group. Collectively, these results suggest that ALR is involved in the response to nitrosative stress by regulating GSH/NO levels in the swamp eel.

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“…In sea bass and swamp eel, the highest transcription level of HO-1 was observed in the liver and was lower in other tissues [32,33]. Similarly, in golden pompano, the transcriptional level of HO-1 was considerably high in the liver [34].…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Heme Oxygenase-1 from Litopenaeus vannamei Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress

Huang

Yang

et al. 2022

Fishes

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Heme oxygenase-1 (HO-1) is a stress-inducible enzyme with antioxidant, anti-inflammatory, and anti-apoptotic effects. In this study, the HO-1 gene from Litopenaeus vannamei (Lv-HO-1) was identified. The open reading frame of Lv-HO-1 is 747 bp, encoding a peptide of 248 amino acids as well as a conserved HemO structural domain. Lv-HO-1 is 70–90% homological to crustaceans and about 50% homological to arthropods. The transcript levels of Lv-HO-1 were highest in the hepatopancreas and lower in other tissues. Knockdown of Lv-HO-1 led to structural destruction of the hepatopancreas. After ammonia exposure, Lv-HO-1 was significantly induced. Knockdown of Lv-HO-1 during ammonia exposure resulted in a significant decrease in antioxidant capacity and cellular autophagy levels compared to the control and increased apoptosis. The transcriptional levels of SOD and GSH-Px were considerably reduced (p < 0.05), as were the transcriptional levels of Atg3, Atg4, Atg5, and Atg10. The results indicated that Lv-HO-1 from L. vannamei can be induced by oxidative stress and may have important roles in regulating the host antioxidant system, reducing cell apoptosis.

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Heme oxygenase 1 plays a crucial role in swamp eel response to oxidative stress induced by cadmium exposure or Aeromonas hydrophila infection

Cited by 13 publications

References 57 publications

Revealing the antibacterial power of hydrogen-releasing PdH nanohydride against drug resistant Staphylococcus aureus: an in-depth mechanism study

Revealing the antibacterial power of hydrogen-releasing PdH nanohydride against drug resistant Staphylococcus aureus: an in-depth mechanism study

Swamp eel aldehyde reductase is involved in response to nitrosative stress via regulating NO/GSH levels

Identification and Characterization of Heme Oxygenase-1 from Litopenaeus vannamei Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress

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