Heme oxygenase expression as a biomarker of exposure to amphiphilic polymer-coated CdSe/ZnS quantum dots

McConnachie, Lisa A.; White, Collin C.; Botta, Dianne; Zadworny, Megan E.; Cox, David A.; Beyer, Richard P.; Hu, Xiaoge; Eaton, David L.; Gao, Xiaohu; Kavanagh, Terrance J.

doi:10.3109/17435390.2011.648224

Cited by 22 publications

(31 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There was essentially no change in cell membrane integrity using Trypan Blue for all three PDots (Figure 3, panel B), while the positive control with 0.2nM Etoposide showed a 30% decrease. These results indicated that PDots do not substantially affect cell viability for doses ranging from 2.5 nM to 40 nM, which is consistent with previously reported results 10,19-21,22 and are comparable to those of QDots previously reported as well 24 .…”

Section: Resultssupporting

confidence: 92%

“…with in vivo exposures. RAW264.7 cells were previously reported to show good uptake capability for QDots 24 . In this study, RAW264.7 cells avidly took up both QDots and PDots as shown in Figure 2.…”

Section: Resultsmentioning

confidence: 99%

“…Amphiphilic polymer coated TOPO-PMAT CdSe/ZnS QDots were prepared as previously reported 23,24 . Briefly, 40 mg of PMAT was mixed with 17.7 nmol of TOPO QDots suspended in chloroform.…”

Section: Methodsmentioning

confidence: 99%

“…Red PDots (PFBT/PFDBT5) (B) QDot preparation and schematic of their structural characteristics. (C) PDot and (D) QDot size determination from TEM and dynamic light scattering properties, 9,24 (E) Structure of NIR720.…”

Section: Figurementioning

confidence: 99%

See 3 more Smart Citations

Toxicity and oxidative stress induced by semiconducting polymer dots in RAW264.7 mouse macrophages

White

Jin

et al. 2015

Nanoscale

Self Cite

View full text Add to dashboard Cite

The rapid development and acceptance of PDots for biological applications depends on an in depth understanding of their cytotoxicity. In this paper, we performed a comprehensive study of PDot cytotoxicity at both the gross cell effect level (such as cell viability, proliferation and necrosis) and more subtle effects (such as redox stress) on RAW264.7 cells, a murine macrophage cell line with high relevance to in vivo nanoparticle disposition. The redox stress measurements assessed were inner mitochondrial membrane lipid peroxidation (nonyl-acridine orange, NAO), total thiol level (monobromobimane, MBB), and pyridine nucleotide redox status (NAD(P)H autofluorescence). Because of the extensive work already performed with QDots on nanotoxicity and also because of their comparable size, QDots were chosen as a comparison/reference nanoparticle for this study. The results showed that PDots exhibit cytotoxic effects to a much lesser degree than their inorganic analogue (QDots) and are much brighter, allowing for much lower concentrations to be used in various biological applications. In addition, at lower dose levels (2.5 nM to 10 nM) PDot treatment resulted in higher total thiol level than those found with QDots. At higher dose levels (20 nM to 40 nM) QDots caused significantly higher thiol levels in RAW264.7 cells, than was seen with PDots, suggesting that QDots elicit compensation to oxidative stress by upregulating GSH synthesis. At the higher concentrations of QDots, NAD(P)H levels showed an initial depletion, then repletion to a level that was greater than vehicle controls. PDots showed a similar trend but this was not statistically significant. Because PDots elicit less oxidative stress and cytotoxicity at low concentrations than QDots, and because they exhibit superior fluorescence at these low concentrations, PDots are predicted to have enhanced utility in biomedical applications.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

“…Amphiphilic polymer coated TOPO-PMAT CdSe/ZnS QDots were prepared as previously reported 23,24 . Briefly, 40 mg of PMAT was mixed with 17.7 nmol of TOPO QDots suspended in chloroform.…”

Section: Methodsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

See 2 more Smart Citations

Toxicity and oxidative stress induced by semiconducting polymer dots in RAW264.7 mouse macrophages

White

Jin

et al. 2015

Nanoscale

Self Cite

View full text Add to dashboard Cite

show abstract

“…Pulmonary HO-1 levels were reported to be an effective marker of oxidative stress (Ayres et al, 2008;Li et al, 2009), and prior in vitro research indicated HO-1 as a biomarker of exposure to quantum dot nanoparticles, which elicit their acute toxicity via generation of reactive oxygen intermediates (McConnachie et al, 2013). Further, evidence from in vivo studies utilizing chronic UF ambient particle exposure demonstrated activation of the Nrf-2 signaling pathway, a regulator of HO-1 and a pathway subject to diminished upregulation with aging that might potentially explain additional susceptibility in the aging population to PM pollution (Zhang et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Allergic Airway Inflammation is Differentially Exacerbated by Daytime and Nighttime Ultrafine and Submicron Fine Ambient Particles: Heme Oxygenase-1 as an Indicator of PM-Mediated Allergic Inflammation

Carosino

Bein

Plummer

et al. 2015

Journal of Toxicology and Environmental Health, Part A

View full text Add to dashboard Cite

Ambient particulate matter (PM) originates from a range of sources and differs in composition with respect to season, time of day, and particle size. In this study, ambient PM samples in the ultrafine and submicrometer fine range were tested for the potential to exacerbate a murine model of allergic airway inflammation when exposure occurs solely during allergic sensitization, but not during subsequent allergen challenge. Temporally resolved and size-segregated PM samples were used to understand how summer or winter, day or night, and ambient ultrafine and submicrometer fine particle size influence PM's ability to exacerbate allergic inflammation. PM was collected in urban Fresno, CA. BALB/c mice were exposed to PM and house dust mite allergen (HDM) via intranasal aspiration on d 1, 3, and 5. HDM challenge occurred on d 12-14, with inflammation assessed 24 h following final challenge. While season or particle size did not predict allergic inflammation, daytime ultrafine and submicrometer fine particles significantly increased total cellular inflammation, specifically lymphocyte and eosinophil infiltration, compared to allergic controls. Further studies examined PM-mediated changes within the lung during the period where allergen sensitization occurred by measuring direct effects of PM on pulmonary oxidative stress and inflammation. Pulmonary levels of heme oxygenase-1 (HO-1), a biomarker of oxidative stress, but not cellular inflammation, demonstrated a remarkable correlation with the degree of allergic inflammation in animals sensitized to allergen and PM concomitantly, suggesting acute PM-mediated HO-1 levels may serve as a predictive indicator of a particle's ability to exacerbate allergic airway inflammation.

show abstract

Carbon‐Dot‐Based Two‐Photon Visible Nanocarriers for Safe and Highly Efficient Delivery of siRNA and DNA

Wang

Bhirde

et al. 2014

Adv Healthcare Materials

View full text Add to dashboard Cite

The emergence of photoluminescent carbon-based nanomaterials offers great potential for a wide variety of biomedical applications such as fluorescence imaging and cellular labeling. This report illustrates a novel photonic carbon dot (Cdots) based nanocarrier by using low molecular weight amphiphilic PEI (Alkyl-PEI2k) for surface passivation. The resulting water-dispersible Alkyl-PEI2k-Cdot nanocarrier possesses good stability, monodispersity with narrow size distribution and fluorescence properties. In addition, Alkyl-PEI2k-Cdot nanocarrier has a markedly low toxicity and good gene transfection effect in vitro and in vivo. Considering its low cytotoxicity, high gene delivery efficiency and fluorescence performance, Alkyl-PEI2k-Cdots could serve as a novel imaging-trackable gene-delivery nanocarrier promising for gene therapy and optical molecular imaging.

show abstract

Heme oxygenase expression as a biomarker of exposure to amphiphilic polymer-coated CdSe/ZnS quantum dots

Cited by 22 publications

References 59 publications

Toxicity and oxidative stress induced by semiconducting polymer dots in RAW264.7 mouse macrophages

Toxicity and oxidative stress induced by semiconducting polymer dots in RAW264.7 mouse macrophages

Allergic Airway Inflammation is Differentially Exacerbated by Daytime and Nighttime Ultrafine and Submicron Fine Ambient Particles: Heme Oxygenase-1 as an Indicator of PM-Mediated Allergic Inflammation

Carbon‐Dot‐Based Two‐Photon Visible Nanocarriers for Safe and Highly Efficient Delivery of siRNA and DNA

Contact Info

Product

Resources

About