Hemocytin, the special aggregation factor connecting insect hemolymph immunity, a potential target of insecticidal immunosuppresant

Hu, Hongwang; Hu, Qiongbo; Weng, Qunfang; Wang, Jingjing

doi:10.1016/j.pestbp.2023.105704

Cited by 4 publications

(3 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These two EPFs are considered the generalist Metarhizium , which has broad host spectra in relation to the specialist M. acridum with the single host. The generalist Metarhizium acquired destruxins, a cyclopeptidic mycotoxin, which is an insecticidal compound with multiple bioactivities such as anti-immunity, anti-feeding, and growth regulation [ 25 , 34 , 35 ]. The results suggest that the two Metarhizium species primarily cause insect disease through the digestive tract.…”

Section: Discussionmentioning

confidence: 99%

Mycophagous Mite, Tyrophagus putrescentiae, Prefers to Feed on Entomopathogenic Fungi, except Metarhizium Generalists

Ou,

Chen,

et al. 2024

Microorganisms

Self Cite

View full text Add to dashboard Cite

(1) Background: The mycophagous mite, Tyrophagus putrescentiae, was found to feed on entomopathogenic fungi (EPF) in our previous experiments, which seriously impacted the culture and preservation of fungal strains. Therefore, it is necessary to investigate the biological characteristics of the occurrence and damage to EPF. (2) Methods: The mite’s growth and development and feeding preference were surveyed by comparative culture and observation; also, optical and electronic microscopies were employed. (3) Results: T. putrescentiae could survive normally after being fed on seven EPF species, including Purpureocillium lilacinum, Marquandii marquandii, Cordyceps fumosorosea, Beauveria bassiana, Metarhizium flavoviride, Lecanicillium dimorphum, and Metacordyceps chlamydosporia. The first four fungi were the mite’s favorites with their greater feeding amount and shorter developmental duration. Interestingly, the mite could also feed on Metarhizium anisopliae and Metarhizium robertsii, but this led to the mite’s death. After feeding on M. anisopliae and M. robertsii, the mites began to die after 24 h, and the mortality rate reached 100% by 72 h. Observation under optical microscopy and scanning electron microscopy revealed that the conidia of M. anisopliae and M. robertsii adhered to the mite’s surface, but there was no evidence of penetration or invasion. However, dissection observation indicated that the two Metarhizium species germinate and grow within the mite’s digestive tract, which implies that Metarhizium generalists with broad-spectrum hosts and the production of destruxins have acaricidal activity toward the mycophagous mites.

show abstract

Section: Discussionmentioning

confidence: 99%

Mycophagous Mite, Tyrophagus putrescentiae, Prefers to Feed on Entomopathogenic Fungi, except Metarhizium Generalists

Ou,

Chen,

et al. 2024

Microorganisms

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the hemocyte-poor hemolymph, as in butterflies and moths, the humoral reactions in the plasma cause the self-assembly of lipoproteins (Bohn and Brehelin, 1986;Zdybicka-Barabas and Cytrynska, 2013;Ling et al, 2018;Miserez et al, 2023). Lipoproteins, with their surfactant-like action, recognize the foreign interface and immediately engage hemocytes or other large proteins, such as hemocytin (molecular weights of hemocytin in the moth Bombyx mori and the fly Drosophila melanogaster are ~260 and ~300 kDa, respectively) (Hu et al, 2024), to aggregate into filamentary threads consisting of more complex biopolymer subunits (Mulnix et al, 1995) and then into gel-like wound covers (Gregoire and Rockstein, 1974;Bohn and Brehelin, 1986). The gel tightening of the hemocytes at the wound surface renders the complex mesh impermeable to microorganisms and hinders water evaporation.…”

Section: Introductionmentioning

confidence: 99%

“…In contrast to the hemocyte-poor blood of butterflies and moths (Lepidoptera), the hemolymph of their larvae (i.e., caterpillars) is hemocyte-rich. The comparative biomolecular studies of clotting in lepidopteran larvae and adults and in other insects suggest that the hemocytin and lipoproteins common in adults and larvae could be among the first to respond and trigger complex immune reactions, resulting in the formation of filamentary structures and primary clots (Boman and Hultmark, 1987;Mulnix et al, 1995;Theopold et al, 2004;Rahman et al, 2006;Jiang et al, 2010;Zdybicka-Barabas and Cytrynska, 2013;Staczek et al, 2018;Maravilla et al, 2020;Hu et al, 2024).…”

Section: Introductionmentioning

confidence: 99%

To seal a wound, caterpillars transform blood from a viscous to a viscoelastic fluid in a few seconds

Aprelev,

Brasovs,

Bruce

et al. 2024

Front. Soft Matter

View full text Add to dashboard Cite

In insects vulnerable to dehydration, the mechanistic reaction of blood after wounding is rapid. It allows insects to minimize blood loss by sealing the wound and forming primary clots that provide scaffolding for the formation of new tissue. Using nano-rheological magnetic rotational spectroscopy with nickel nanorods and extensional rheology, we studied the properties of blood dripping from the wound of caterpillars of the Carolina sphinx moth (Manduca sexta) with a high concentration of blood cells. We discovered that wound sealing followed a two-step scenario. First, in a few seconds, the Newtonian low-viscosity blood turns into a non-Newtonian viscoelastic fluid that minimizes blood loss by retracting the dripping blood back into the wound. Next, blood cells aggregate, starting from the interfaces and propagating inward. We studied these processes using optical phase-contrast and polarized microscopy, X-ray imaging, and modeling. Comparative analyses of the cell-rich and cell-poor blood of different insects revealed common features of blood behavior. These discoveries can help design fast-working thickeners for vertebrate blood, including human blood.

show abstract

The parasitoid Exorista sorbillans exploits host silkworm encapsulation to build respiratory funnel for survival

Xu,

Lu,

et al. 2024

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

Hemocytin, the special aggregation factor connecting insect hemolymph immunity, a potential target of insecticidal immunosuppresant

Cited by 4 publications

References 69 publications

Mycophagous Mite, Tyrophagus putrescentiae, Prefers to Feed on Entomopathogenic Fungi, except Metarhizium Generalists

Mycophagous Mite, Tyrophagus putrescentiae, Prefers to Feed on Entomopathogenic Fungi, except Metarhizium Generalists

To seal a wound, caterpillars transform blood from a viscous to a viscoelastic fluid in a few seconds

The parasitoid Exorista sorbillans exploits host silkworm encapsulation to build respiratory funnel for survival

Contact Info

Product

Resources

About