Hemodialysis Effect on the Composition of the Eye Fluid of Cataract Patients

Abstract

Numerous reports have proven that dialysis patients experience disturbances in the levels of elements in biological fluids. Disturbances in the homeostasis of essential elements or the appearance of highly toxic elements are serious problems also in clinical ophthalmology. The purpose of this study was to investigate the influence of hemodialysis (HD) on the elemental composition of anterior chamber aqueous humor (AH) in patients undergoing cataract surgery. The study involved 22 patients. The control group enrolled 16 patients (age 75.68 ± 9.67, female 54.55%, male 45.45%) with cataract and normal kidney function (control), and the second group included six patients (age 70.33 ± 12.74, female 33.33%, male 66.67%) with cataract undergoing HD treatment. The elements quantification was established using an inductively coupled plasma optical emission spectrometer (ICP-MS). In the eye fluid of dialysis patients, there were increased levels of manganese (Mn) and mercury (Hg) and decreased levels of vanadium (V) and zinc (Zn). In addition, a statistically significant increase in the Hg/Zn and Hg/selenium (Se) ratios and a lowering of the iron (Fe)/Mn ratio were observed in the studied group in comparison to the control. The obtained results indicated the need for Zn and Se supplementation in order to eliminate the hazards caused by Hg toxicity. A lower level of V in the eye fluid of dialysis patients may have a positive effect on maintaining a calcium and phosphorus homeostasis. Our study gives a deep insight into changes of elements concentrations in AH induced by HD.

Chronic Alcohol Abuse Alters Hepatic Trace Element Concentrations-Metallomic Study of Hepatic Elemental Composition by Means of ICP-OES

“…Although these correlations have not reached statistical significance, they shed new light on the complex processes of homeostasis in trace element metabolism. So far, such intercorrelations have been tracked by our team in body fluids such as fluid from the anterior chamber of the human eye [ 42 , 43 ], and in tissues collected post mortem from various sites of the human brain [ 12 , 13 , 44 , 45 ]. Our research to date has confirmed that in the case of disturbance of physiological processes that take place in eye diseases (cataracts), AUD, or are the result of medical procedures such as hemodialysis [ 44 ], there are visible changes in the intercorrelation matrices.…”

“…So far, such intercorrelations have been tracked by our team in body fluids such as fluid from the anterior chamber of the human eye [ 42 , 43 ], and in tissues collected post mortem from various sites of the human brain [ 12 , 13 , 44 , 45 ]. Our research to date has confirmed that in the case of disturbance of physiological processes that take place in eye diseases (cataracts), AUD, or are the result of medical procedures such as hemodialysis [ 44 ], there are visible changes in the intercorrelation matrices. In the present study, it can be seen that most elements evaluated in tissues collected from the AUDs of patients are related to each other by strong positive correlations represented by Spearman’s r coefficient.…”

ICP-MS Multi-Elemental Analysis of the Human Meninges Collected from Sudden Death Victims in South-Eastern Poland

“…Without doubt, the lanthanides have received little attention so far when it comes to analyzing the elemental composition of human tissues. In our previous reports, we have shown their presence in fluid collected from the anterior chamber of patients undergoing cataract surgery [ 38 , 91 , 92 ]. This fluid, which is partly a plasma filtrate, had a lanthanide content that was two or three orders of magnitude lower than in the meninges.…”

The First Evidence on the Occurrence of Bisphenol Analogues in the Aqueous Humor of Patients Undergoing Cataract Surgery

“…For this purpose, the composition of amino acid and lipid metabolites such as glutaric acid and pelargonic acid [ 7 ], markers of lipid peroxidation [ 8 ], enzymatic antioxidants such as superoxide dismutase (SOD) and catalase (CAT) [ 8 ], and tryptophan metabolites [ 9 ] were investigated. Due to the small amount of material for research, restrictions related to research on humans, and trace amounts of analytes in AH, advanced analytical techniques are used for analysis, such as nano-LC-ESI-MS/MS [ 10 ], ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry with electrospray ionization (UHPLC-ESI-MS/MS) [ 9 ], ICP-OES [ 11 ], ICP-MS [ 12 ].…”