Hemodynamic shear stress stimulates migration and extravasation of tumor cells by elevating cellular oxidative level

Ma, Shijun; Fu, Afu; Chiew, Geraldine Giap Ying; Luo, Kathy Qian

doi:10.1016/j.canlet.2016.12.001

Cited by 80 publications

(95 citation statements)

References 37 publications

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“…Intracellular and nuclear DOX accumulation after drug release in MDA‐MB‐231 cells was studied using fluorescence imaging. MDA‐MB‐231 cells were grown for 24 hours and treated with or without free DOX, N‐GQD‐DOX and N‐GQD‐DOX‐APTES in FluoroBrite medium (Thermo Fisher Scientific) containing 10% FBS (Hyclone, Buckinghamshire, UK) for 24 hours . Hoechst staining solution (16.23 mM) was created by diluting the Hoechst stock solution in PBS with a ratio of 1:2000.…”

Section: Methodsmentioning

confidence: 98%

Graphene quantum dot based charge‐reversal nanomaterial for nucleus‐targeted drug delivery and efficiency controllable photodynamic therapy

Regmi

et al. 2019

Journal of Biophotonics

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Graphene quantum dots (GQDs), the new zero‐dimensional carbon nanomaterial, have been demonstrated as a promising material for biomedical applications due to its good biocompatibility and low toxicity. However, the integration of multiple therapeutic approaches into a nanosized platform based on the GQD has not been explored yet to our best knowledge. In this report, we regulate the generation of reactive oxygen species (ROS) when using the GQD as a photosensitizer by varying the doping amount of nitrogen atoms to achieve efficiency controllable photodynamic therapy. On the other hand, charge‐reversal (3‐Aminopropyl) triethoxysilane (APTES) was used to conjugate on the surface of GQD for nucleus targeting drug delivery for the first time. The treatment outcome of produced ROS and nucleus‐targeting drug delivery was investigated by fluorescence imaging. The results demonstrated that the N‐GQD‐DOX‐APTES in dual roles as a drug carrier and photosensitizer could achieve nucleus‐targeting delivery and strong ROS production simultaneously. This approach provides a promising strategy for the development of multifunctional therapy in one nano platform for biomedical applications.

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Section: Methodsmentioning

confidence: 98%

Graphene quantum dot based charge‐reversal nanomaterial for nucleus‐targeted drug delivery and efficiency controllable photodynamic therapy

Regmi

et al. 2019

Journal of Biophotonics

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“…Furthermore, the mechanism studied showed that the shear stress induced the ROS production in breast cancer cells [267]. Interestingly the combined effect of shear stress enhances the ROS production in DOX and cisplatin-treated cells with no effects in Taxol and etoposide-treated cells.…”

Section: Discussionmentioning

confidence: 97%

“…The second mechanism is to produce superoxide, which can damage mitochondria and induce apoptosis [230,306]. In our previous study, we showed that pulsatile SS elevated the levels of ROS, including superoxide and hydrogen peroxide, in circulated breast cancer cells [230,267]. We thus hypothesized that these SS-elevated ROS can further increase the apoptotic effect of DOX.…”

Section: Physiological Fluidic Shear Stress Significantly Enhanced Thmentioning

confidence: 96%

“…Thus, the intensity of green fluorescence can indicate the level of ROS [242]. Using this dye, we previously showed that circulating MDA-MB-231 cells under SS15 for 1-6 h significantly elevated the intracellular level of ROS [230,267].…”

Section: Shear Stress Elevated the Levels Of Ros In Dox-and Cisplatinmentioning

confidence: 99%

“…in our earlier publication [143,230,266,267]. The goal is to validate the shear stress experienced by cancer cells in the experimental work of our microfluidic system with a numerical simulation using a COMSOL multi-physics software.…”

Section: Shear Stress Was Experimentally Calculated Using the Poiseuimentioning

confidence: 99%

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Study of the destructive effects of shear stress and ROS-generating drugs on circulating tumor cells

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Vascularized Biomaterials to Study Cancer Metastasis

Bittner

Jiménez

Peyton

2020

Adv Healthcare Materials

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Cancer metastasis, the spread of cancer cells to distant organs, is responsible for 90% of cancer‐related deaths. Cancer cells need to enter and exit circulation in order to form metastases, and the vasculature and endothelial cells are key regulators of this process. While vascularized 3D in vitro systems have been developed, few have been used to study cancer, and many lack key features of vessels that are necessary to study metastasis. This review focuses on current methods of vascularizing biomaterials for the study of cancer, and three main factors that regulate intravasation and extravasation: endothelial cell heterogeneity, hemodynamics, and the extracellular matrix of the perivascular niche.

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Hemodynamic shear stress stimulates migration and extravasation of tumor cells by elevating cellular oxidative level

Cited by 80 publications

References 37 publications

Graphene quantum dot based charge‐reversal nanomaterial for nucleus‐targeted drug delivery and efficiency controllable photodynamic therapy

Graphene quantum dot based charge‐reversal nanomaterial for nucleus‐targeted drug delivery and efficiency controllable photodynamic therapy

Study of the destructive effects of shear stress and ROS-generating drugs on circulating tumor cells

Vascularized Biomaterials to Study Cancer Metastasis

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