Hemoglobin and hemin induce DNA damage in human colon tumor cells HT29 clone 19A and in primary human colonocytes

Glei, Michael; Klenow, Stefanie; Sauer, Julia; Wegewitz, Uta; Richter, Konrad Klaus; Pool‐Zobel, Beatrice L.

doi:10.1016/j.mrfmmm.2005.08.006

Cited by 85 publications

(50 citation statements)

References 34 publications

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“…These include the induction of comet effects by apoptosis inducers which inhibit topoisomerases (Boos & Stopper, 2000;Gieseler et al, 1999); cytokine treatment of cultured cells (Delaney et al, 1997); sodium dodecyl sulfate and potassium cyanide (Henderson et al, 1998); colchicine, dl-menthol and sodium acetate (Kiffe et al, 2003); luteolin (Michels et al, 2005); gossypol (Quintana et al, 2000), carbon tetrachloride (Sasaki et al, 1998) and vitamin C (Anderson et al, 1994). Further examples of induction of comet effects of questionable genotoxic biological significance include dietary flavonoids quercetin, myricetin and silymarin (Duthie et al, 1997); hemoglobin (Glei et al, 2006); olive oil extracts (Nousis et al, 2005) and capsaicin (Richeux et al, 1999).…”

Section: Significance Of Dna Damage Endpoint Resultsmentioning

confidence: 99%

Review of genotoxicity studies of glyphosate and glyphosate-based formulations

Kier

Kirkland²

2013

Critical Reviews in Toxicology

120

102

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An earlier review of the toxicity of glyphosate and the original Roundupä-branded formulation concluded that neither glyphosate nor the formulation poses a risk for the production of heritable/somatic mutations in humans. The present review of subsequent genotoxicity publications and regulatory studies of glyphosate and glyphosate-based formulations (GBFs) incorporates all of the findings into a weight of evidence for genotoxicity. An overwhelming preponderance of negative results in well-conducted bacterial reversion and in vivo mammalian micronucleus and chromosomal aberration assays indicates that glyphosate and typical GBFs are not genotoxic in these core assays. Negative results for in vitro gene mutation and a majority of negative results for chromosomal effect assays in mammalian cells add to the weight of evidence that glyphosate is not typically genotoxic for these endpoints in mammalian systems. Mixed results were observed for micronucleus assays of GBFs in nonmammalian systems. Reports of positive results for DNA damage endpoints indicate that glyphosate and GBFs tend to elicit DNA damage effects at high or toxic dose levels, but the data suggest that this is due to cytotoxicity rather than DNA interaction with GBF activity perhaps associated with the surfactants present in many GBFs. Glyphosate and typical GBFs do not appear to present significant genotoxic risk under normal conditions of human or environmental exposures.

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Section: Significance Of Dna Damage Endpoint Resultsmentioning

confidence: 99%

Review of genotoxicity studies of glyphosate and glyphosate-based formulations

Kier

Kirkland²

2013

Critical Reviews in Toxicology

120

102

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“…Cigarette smoking has been identified as one of the main factors affecting DNA damage in nurses handling cytostatic drugs 30,31) . Age, gender, air pollution, diet, infection, sunlight, Hb, and residential radon have also been regarded as other possible factors affecting the level of DNA damage detected in the comet assay 21,32) , but there is no evidence to suggest DNA damage due to shift work. Actually, we used most of these confounders, as well as hospital, in the data analysis, and one trained examiner conducted all biochemical tests under masking to avoid a measurement bias.…”

Section: Discussionmentioning

confidence: 99%

Assessment of DNA Damage in Japanese Nurses Handling Antineoplastic Drugs by the Comet Assay

Sasaki

Dakeishi

Hoshi

et al. 2008

Journal of Occupational Health

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Assessment of DNA Damage in Japanese Nurses Handling Antineoplastic Drugs by the Comet Assay: Makiko SASAKI, et al. Department of Environmental Health Sciences, Akita University School of Medicine-To clarify genotoxic effects of occupational exposure to antineoplastic drugs in Japan, we examined DNA damage, assessed by the comet assay, in 121 female nurses and 46 female clerks working at three hospitals in the northeast of Japan. The comet assay is considered to be a sensitive and rapid method for DNA strand break detection in individual cells, and tail length and tail moment are used as the comet parameters. Concerning the basal characteristics, the 46 control subjects had higher rates of smoking and coffee-drinking habits and lower hemoglobin than the 121 nurses (p<0.05). The logtransformed tail length in the nurses was significantly longer than that in the control subjects after adjusting for possible covariates such as age and smoking habit (p<0.05). Also, the log-transformed tail length was significantly longer, in the 57 nurses who had handled antineoplastic drugs in the last six months, than that in the 46 control subjects (p<0.05); but, no significant difference in tail length or tail moment was seen between the two nurse groups with and without experience of handling hazardous drugs (p>0.05). These results suggest that Japanese nurses who have worked at hospitals using antineoplastic drugs may have a potential risk of DNA damage. To minimize this risk in Japan, use of biological safety cabinet and appropriate protective equipments, in addition to staff education and training, should be implemented in the healthcare environment. (J Occup Health 2008; 50: 7-12)

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“…This mechanism has received limited support from studies on cancer cells in vitro. They show that hemin induces DNA damage in human cells of colonic origin (Glei, et al, 2006), via hydrogen peroxide produced by hemeoxygenase, which can be inhibited in vitro by Zn-protoporphyrin (Ishikawa, Tamaki, Ohata, Arihara, & Itoh, 2010).…”

Section: The Heme Iron Hypothesis: Fat Peroxidation and N-nitroso Patmentioning

confidence: 99%

Red meat and colon cancer: Should we become vegetarians, or can we make meat safer?

2011

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Hemoglobin and hemin induce DNA damage in human colon tumor cells HT29 clone 19A and in primary human colonocytes

Cited by 85 publications

References 34 publications

Review of genotoxicity studies of glyphosate and glyphosate-based formulations

Review of genotoxicity studies of glyphosate and glyphosate-based formulations

Assessment of DNA Damage in Japanese Nurses Handling Antineoplastic Drugs by the Comet Assay

Red meat and colon cancer: Should we become vegetarians, or can we make meat safer?

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