Hemoglobin-based oxygen carriers: Clinical application of HBOC-201 as an alternative to red blood cells for machine perfusion in liver transplantation

Moein, Mahmoudreza; Ahmed, Maham; Arafa, Fatema; Saidi, Reza F.

doi:10.1016/j.sipas.2022.100120

Cited by 3 publications

(3 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, a variety of alternatives have been proposed ranging from artificial oxygen carriers such as polymerized bovine hemoglobin-based oxygen carriers and pyridoxylated bovine hemoglobin to acellular oxygen-carrying media such as STEEN ( 60 , 61 ). These modern solutions also offer the advantage of convenient storage and transport—at similar effectiveness and rheological-hemodynamic characteristics ( 62 , 63 ).…”

Section: Ex Situ Machine Perfusionmentioning

confidence: 99%

Pushing the boundaries of innovation: the potential of ex vivo organ perfusion from an interdisciplinary point of view

Iske,

Schroeter,

Knoedler

et al. 2023

Front. Cardiovasc. Med.

View full text Add to dashboard Cite

Ex vivo machine perfusion (EVMP) is an emerging technique for preserving explanted solid organs with primary application in allogeneic organ transplantation. EVMP has been established as an alternative to the standard of care static-cold preservation, allowing for prolonged preservation and real-time monitoring of organ quality while reducing/preventing ischemia–reperfusion injury. Moreover, it has paved the way to involve expanded criteria donors, e.g., after circulatory death, thus expanding the donor organ pool. Ongoing improvements in EVMP protocols, especially expanding the duration of preservation, paved the way for its broader application, in particular for reconditioning and modification of diseased organs and tumor and infection therapies and regenerative approaches. Moreover, implementing EVMP for in vivo-like preclinical studies improving disease modeling raises significant interest, while providing an ideal interface for bioengineering and genetic manipulation. These approaches can be applied not only in an allogeneic and xenogeneic transplant setting but also in an autologous setting, where patients can be on temporary organ support while the diseased organs are treated ex vivo, followed by reimplantation of the cured organ. This review provides a comprehensive overview of the differences and similarities in abdominal (kidney and liver) and thoracic (lung and heart) EVMP, focusing on the organ-specific components and preservation techniques, specifically on the composition of perfusion solutions and their supplements and perfusion temperatures and flow conditions. Novel treatment opportunities beyond organ transplantation and limitations of abdominal and thoracic EVMP are delineated to identify complementary interdisciplinary approaches for the application and development of this technique.

show abstract

Section: Ex Situ Machine Perfusionmentioning

confidence: 99%

Pushing the boundaries of innovation: the potential of ex vivo organ perfusion from an interdisciplinary point of view

Iske,

Schroeter,

Knoedler

et al. 2023

Front. Cardiovasc. Med.

View full text Add to dashboard Cite

show abstract

“…Simultaneously, different perfusate mediums can be altered to supply the desired amounts of oxygen and nutrients. For example, Normothermic Machine Perfusion (NMP), an FDA approved protocol, involves maintaining the organ at a physiological temperature of 37 °C and supplementing the perfusate with an oxygen carrier, such as packed red blood cells (pRBC) 11 , although other synthetic oxygen carriers have also been used experimentally 12 , 13 . While NMP minimizes cold ischemia time, grafts are continuously maintained in a hyper-oxygenated state 14 .…”

Section: Introductionmentioning

confidence: 99%

Real-time monitoring of mitochondrial oxygenation during machine perfusion using resonance Raman spectroscopy predicts organ function

Jain,

Ajenu,

Lopera Higuita

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Organ transplantation is a life-saving procedure affecting over 100,000 people on the transplant waitlist. Ischemia reperfusion injury (IRI) is a major challenge in the field as it can cause post-transplantation complications and limit the use of organs from extended criteria donors. Machine perfusion technology has the potential to mitigate IRI; however, it currently fails to achieve its full potential due to a lack of highly sensitive and specific assays to assess organ quality during perfusion. We developed a real-time and non-invasive method of assessing organs during perfusion based on mitochondrial function and injury using resonance Raman spectroscopy. It uses a 441 nm laser and a high-resolution spectrometer to quantify the oxidation state of mitochondrial cytochromes during perfusion. This index of mitochondrial oxidation, or 3RMR, was used to understand differences in mitochondrial recovery of cold ischemic rodent livers during machine perfusion at normothermic temperatures with an acellular versus cellular perfusate. Measurement of the mitochondrial oxidation revealed that there was no difference in 3RMR of fresh livers as a function of normothermic perfusion when comparing acellular versus cellular-based perfusates. However, following 24 h of static cold storage, 3RMR returned to baseline faster with a cellular-based perfusate, yet 3RMR progressively increased during perfusion, indicating injury may develop over time. Thus, this study emphasizes the need for further refinement of a reoxygenation strategy during normothermic machine perfusion that considers cold ischemia durations, gradual recovery/rewarming, and risk of hemolysis.

show abstract

“…Simultaneously, different perfusate mediums can be altered to supply the desired amounts of oxygen and nutrients. For example, Normothermic Machine Perfusion (NMP), an FDA approved protocol, involves maintaining the organ at a physiological temperature of 37°C and supplementing the perfusate with an oxygen carrier, such as packed red blood cells (pRBC) 11 or synthetic hemoglobin from swine (HBOC201 19 ) and murine sources (Hemarina M101 20 ). While NMP minimizes ischemia time, grafts are continuously maintained in a hyper-oxygenated state 12 .…”

Section: Introductionmentioning

confidence: 99%

Real-time monitoring of mitochondrial oxygenation during machine perfusion using resonance Raman spectroscopy predicts organ function

Jain,

Ajenu,

Hafiz

et al. 2023

Preprint

View full text Add to dashboard Cite

Organ transplantation is a life-saving procedure affecting over 100,000 people on the transplant waitlist. Ischemia reperfusion injury is a major challenge in the field as it can cause post-transplantation complications and limits the use of organs from extended criteria donors. Machine perfusion technology is used to repair organs before transplant, however, currently fails to achieve its full potential due to a lack of highly sensitive and specific assays to predict organ quality during perfusion. We developed a real-time and non-invasive method of assessing organ function and injury based on mitochondrial oxygenation using resonance Raman spectroscopy. It uses a 441 nm laser and a high-resolution spectrometer to predict the oxidation state of mitochondrial cytochromes during perfusion, which vary due to differences in storage compositions and perfusate compositions. This index of mitochondrial oxidation, or 3RMR, was found to predict organ health based on clinically utilized markers of perfusion quality, tissue metabolism, and organ injury. It also revealed differences in oxygenation with perfusates that may or may not be supplemented with packed red blood cells as oxygen carriers. This study emphasizes the need for further refinement of a reoxygenation strategy during machine perfusion that is based on a gradual recovery from storage. Thus, we present a novel platform that provides a real-time and quantitative assessment of mitochondrial health during machine perfusion of livers, which is easy to translate to the clinic.

show abstract

Hemoglobin-based oxygen carriers: Clinical application of HBOC-201 as an alternative to red blood cells for machine perfusion in liver transplantation

Cited by 3 publications

References 19 publications

Pushing the boundaries of innovation: the potential of ex vivo organ perfusion from an interdisciplinary point of view

Pushing the boundaries of innovation: the potential of ex vivo organ perfusion from an interdisciplinary point of view

Real-time monitoring of mitochondrial oxygenation during machine perfusion using resonance Raman spectroscopy predicts organ function

Real-time monitoring of mitochondrial oxygenation during machine perfusion using resonance Raman spectroscopy predicts organ function

Contact Info

Product

Resources

About