Hemoglobin-modified nanoparticles for electrochemical determination of haptoglobin: Application in bovine mastitis diagnosis

Carinelli, Soledad; Fernández, Iñigo; González–Mora, José Luis; Salazar, Pedro

doi:10.1016/j.microc.2022.107528

Cited by 12 publications

(8 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Once Hp was complexed by the Hb-MB conjugates, it was captured with an anti-bovine Hp antibody further labeled with alkaline phosphatase. The method showed a range of detection from 25 to 800 μg mL −1 and a LOD of 43 ng mL −1 [21]. Importantly, the biosensor developed in our work shows better LOD (70 pg mL −1 ) than those claimed for most of the already reported methodologies for the determination of total Hp without requiring tedious protocols or complex and expensive instrumentation.…”

Section: Immunoplatforms For the Single Determination Of Total And Gl...mentioning

confidence: 64%

“…It is important to note that, nowadays, the reported biosensors for the determination of glycosylated biomarkers mainly use aptamers [13,14] or lectins [15,16] as recognition elements. Moreover, although some electrochemical biodevices have been reported for the determination of total Hp [17][18][19][20][21], to date, no electrochemical immunoplatform has been described for the determination of glycosylated Hp or for total and glycosylated Hp simultaneous determination.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin

et al. 2022

View full text Add to dashboard Cite

The glycosylation status of proteins is increasingly used as biomarker to improve the reliability in the diagnosis and prognosis of diseases as relevant as cancer. This feeds the need for tools that allow its simple and reliable analysis and are compatible with applicability in the clinic. With this objective in mind, this work reports the first bioelectronic immunoplatforms described to date for the determination of glycosylated haptoglobin (Hp) and the simultaneous determination of total and glycosylated Hp. The bioelectronic immunoplatform is based on the implementation of non-competitive bioassays using two different antibodies or an antibody and a lectin on the surface of commercial magnetic microcarriers. The resulting bioconjugates are labeled with the horseradish peroxidase (HRP) enzyme, and after their magnetic capture on disposable electroplatforms, the amperometric transduction using the H2O2/hydroquinone (HQ) system allows the single or multiple detection. The developed immunoplatform achieves limits of detection (LODs) of 0.07 and 0.46 ng mL−1 for total and glycosylated Hp in buffer solution, respectively. The immunoplatform allows accurate determination using simple and relatively short protocols (approx. 75 min) of total and glycosylated Hp in the secretomes of in vitro–cultured colorectal cancer (CRC) cells with different metastatic potentials, which is not feasible, due to lack of sensitivity, by means of some commercial ELISA kits and Western blot methodology. Graphical abstract

show abstract

Section: Immunoplatforms For the Single Determination Of Total And Gl...mentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Figure 2 b shows the Nyquist plot for the GCE/CNT/Au electrode (the results for GCE, GCE/Au, and GCE/CNT electrodes are also illustrated). The impedance spectra of electrochemical sensors typically exhibit two distinct regions: a linear line portion at lower frequencies, which is associated with the diffusion phenomenon, and a semicircular portion at higher frequencies, which is related to the electron transfer process [ 39 ]. The semicircle portion diameter can be used to determine the electron transfer resistance (Rct) at the surface of the sensor [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Detection of Bisphenol A Based on Gold Nanoparticles/Multi-Walled Carbon Nanotubes: Applications on Glassy Carbon and Screen Printed Electrodes

Luis-Sunga,

Carinelli,

García

et al. 2024

Sensors

Self Cite

View full text Add to dashboard Cite

Bisphenol A (BPA) has been classified as an endocrine-disrupting substance that may cause adverse effects on human health and the environment. The development of simple and sensitive electrochemical biosensors is crucial for the rapid and effective quantitative determination of BPA. This work presents a study on electrochemical sensors utilizing gold nanoparticle-modified multi-walled carbon nanotubes (CNT/AuNPs). Glassy carbon electrodes (GCEs) and screen-printed electrodes (SPEs) were conveniently modified and used for BPA detection. AuNPs were electrodeposited onto the CNT-modified electrodes using the galvanostatic method. The electrodes were properly modified and characterized by using Raman spectroscopy, cyclic voltammetry (CV), and electrochemical impedance analysis (EIS). The electrochemical response of the sensors was studied using differential pulse voltammetry (DPV) and constant potential amperometry (CPA) for modified GCE and SPE electrodes, respectively, and the main analytical parameters were studied and optimized. Problems encountered with the use of GCEs, such as sensor degradation and high limit of detection (LOD), were overcome by using modified SPEs and a flow injection device for the measurements. Under this approach, an LOD as low as 5 nM (S/N = 3) was achieved and presented a linear range up to 20 μM. Finally, our investigation addressed interference, reproducibility, and reusability aspects, successfully identifying BPA in both spiked and authentic samples, including commercial and tap waters. These findings underscore the practical applicability of our method for accurate BPA detection in real-world scenarios. Notably, the integration of SPEs and a flow injection device facilitated simplified automation, offering an exceptionally efficient and reliable solution for precise BPA detection in water analysis laboratories.

show abstract

“…Using the enzymatic activity of XOs for the detection of xanthine (Xn), Xn was detected sensitively with a 100 nM LoD and rapid response (within 10 s) using the DPV technique. Additionally, various functional nanocomposite-based enzymatic nanobiosensors have been reported, such as the use of magnetic NPs (MNPs) [ 83 , 84 ]. For instance, the MNP, Iron (III) oxide (Fe 3 O 4 ), encapsulated by polynorepinephrine (PNE), was developed and combined with GOx to develop an enzymatic glucose nanobiosensor operable with a smartphone ( Figure 4 B) [ 84 ].…”

Section: Enzymatic Electrochemical Nanobiosensorsmentioning

confidence: 99%

Enzymatic Electrochemical/Fluorescent Nanobiosensor for Detection of Small Chemicals

Choi

Yoon

2023

Biosensors

View full text Add to dashboard Cite

The detection of small molecules has attracted enormous interest in various fields, including the chemical, biological, and healthcare fields. In order to achieve such detection with high accuracy, up to now, various types of biosensors have been developed. Among those biosensors, enzymatic biosensors have shown excellent sensing performances via their highly specific enzymatic reactions with small chemical molecules. As techniques used to implement the sensing function of such enzymatic biosensors, electrochemical and fluorescence techniques have been mostly used for the detection of small molecules because of their advantages. In addition, through the incorporation of nanotechnologies, the detection property of each technique-based enzymatic nanobiosensors can be improved to measure harmful or important small molecules accurately. This review provides interdisciplinary information related to developing enzymatic nanobiosensors for small molecule detection, such as widely used enzymes, target small molecules, and electrochemical/fluorescence techniques. We expect that this review will provide a broad perspective and well-organized roadmap to develop novel electrochemical and fluorescent enzymatic nanobiosensors.

show abstract

Hemoglobin-modified nanoparticles for electrochemical determination of haptoglobin: Application in bovine mastitis diagnosis

Cited by 12 publications

References 42 publications

First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin

First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin

Electrochemical Detection of Bisphenol A Based on Gold Nanoparticles/Multi-Walled Carbon Nanotubes: Applications on Glassy Carbon and Screen Printed Electrodes

Enzymatic Electrochemical/Fluorescent Nanobiosensor for Detection of Small Chemicals

Contact Info

Product

Resources

About