Hemolytic and cytotoxic activity from cultures of Aureococcus anophagefferens—a causative species of brown tides in the north-western Bohai Sea, China

Huang, Baiqiang; Liang, Yanlan; Pan, Huizhu; Xie, Lei; Jiang, Tao; Jiang, Tianjiu

doi:10.1016/j.chemosphere.2020.125819

Cited by 18 publications

(15 citation statements)

References 45 publications

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“…Dinoflagellates were the dominant phytoplankton assemblages in the study area, according to metabarcoding. Their abundance was comparable with those reported in temperate areas of the northwestern Pacific (Huang et al, 2020;Wu et al, 2020;Liu et al, 2022) and much lower than those in tropical regions, such as the South China Sea (Wang et al, 2022) and the Western Pacific seamount regions (Xu et al, 2021). By comparison, haptophytes were the minor component (0.53% of phytoplankton) in the WSG, but they occupied a relatively high proportion of phytoplankton in the South China Sea (Wang et al, 2022) and the temperate regions of the northwest Pacific controlled by the Kuroshio current (Wu et al, 2020).…”

Section: Phytoplankton Population Structure With An Emphasis On Harmf...supporting

confidence: 65%

“…By comparison, haptophytes were the minor component (0.53% of phytoplankton) in the WSG, but they occupied a relatively high proportion of phytoplankton in the South China Sea (Wang et al, 2022) and the temperate regions of the northwest Pacific controlled by the Kuroshio current (Wu et al, 2020). A high proportion of pelagophytes and cryptophytes was an important characteristic of the WSG when compared with previous studies (Huang et al, 2020;Wu et al, 2020;Xu et al, 2021).…”

Section: Phytoplankton Population Structure With An Emphasis On Harmf...contrasting

confidence: 54%

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Phytoplankton community structure in the Western Subarctic Gyre of the Pacific Ocean during summer determined by a combined approach of HPLC-pigment CHEMTAX and metabarcoding sequencing

Xin

Qin

et al. 2023

Front. Mar. Sci.

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The Western Subarctic Gyre (WSG) is a cyclonic upwelling gyre in the northwest subarctic Pacific, which is a region with a high concentration of nutrients but low chlorophyll. We investigated the community structure and spatial distribution of phytoplankton in this area by using HPLC-pigment CHEMTAX (a chemotaxonomy program) and metabarcoding sequencing during the summer of 2021. The phytoplankton community showed significant differences between the two methods. The CHEMTAX analyses identified eight major marine phytoplankton assemblages. Cryptophytes were the major contributors (24.96%) to the total Chl a, followed by pelagophytes, prymnesiophytes, diatoms, and chlorophytes. The eukaryotic phytoplankton OTUs obtained by metabarcoding were categorized into 149 species in 96 genera of 6 major groups (diatoms, prymnesiophytes, pelagophytes, chlorophytes, cryptophytes, and dinoflagellates). Dinoflagellates were the most abundant group, accounting for 44.74% of the total OTUs obtained, followed by cryptophytes and pelagophytes. Sixteen out of the 97 identified species were annotated as harmful algal species, and Heterocapsa rotundata, Karlodinium veneficum, and Aureococcus anophagefferens were assigned to the abundant group (i.e., at least 0.1% of the total reads). Nutrients were more important in shaping the phytoplankton community than temperature and salinity. The 24 stations were divided into southern and northern regions along 44°N according to the k-means method, with the former being dominated by high Chl a and low nutrients. Although different phytoplankton assemblages analyzed by the two methods showed various relationships with environmental factors, a common feature was that the dinoflagellate proportion showed a significantly negative correlation with low nutrients and a positive correlation with Chl a.

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Section: Phytoplankton Population Structure With An Emphasis On Harmf...supporting

confidence: 65%

Section: Phytoplankton Population Structure With An Emphasis On Harmf...contrasting

confidence: 54%

Phytoplankton community structure in the Western Subarctic Gyre of the Pacific Ocean during summer determined by a combined approach of HPLC-pigment CHEMTAX and metabarcoding sequencing

Xin

Qin

et al. 2023

Front. Mar. Sci.

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“…Aureococcus anophagefferens blooms (also known as brown tides) have caused destructive environmental impacts and massive losses in aquaculture in the past three decades [39][40][41][42]. For a long period, however, brown tides were only reported in the U.S. East Coast until the first brown tides occurred at Saldanha Bay, South Africa in 1997 [6] and suddenly occurred in the coastal waters number of RSCs decreased significantly over time during the storage, which died during the storage because of light or nutrient shortage, or decomposed by bacteria, only about 28% of RSCs survived 3 months of darkness coldness (Figures 4 and 5).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Laboratory Culture-Based Characterization of the Resting Stage Cells of the Brown-Tide-Causing Pelagophyte, Aureococcus anophagefferens

Deng

et al. 2020

JMSE

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Life history (life cycle) plays a vital role in the ecology of some microalgae; however, the well-known brown-tide-causing pelagophyte Aureococcus anophagefferens has been barely investigated in this regard. Recently, based mainly on detections in marine sediments from China, we proved that this organism has a resting stage. We, therefore, conducted a follow-up study to characterize the resting stage cells (RSCs) of A. anophagefferens using the culture CCMP1984. The RSCs were spherical, larger than the vegetative cells, and smooth in cell surface and contained more aggregated plastid but more vacuolar space than vegetative cells. RSCs contained a conspicuous lipid-enriched red droplet. We found a 9.9-fold decrease in adenosine triphosphate (ATP) content from vegetative cells to RSCs, indicative of a "resting" or dormant physiological state. The RSCs stored for 3 months (at 4 °C in darkness) readily reverted back to vegetative growth within 20 days after being transferred to the conditions for routine culture maintenance. Our results indicate that the RSCs of A. anophagefferens are a dormant state that differs from vegetative cells morphologically and physiologically, and that RSCs likely enable the species to survive unfavorable conditions, seed annual blooms, and facilitate its cosmopolitan distribution that we recently documented.

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Diversity and community structure of eukaryotic microalgae in surface sediments in the central Bohai Sea, China, based on a metabarcoding approach

Wang

et al. 2021

J. Ocean. Limnol.

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Hemolytic and cytotoxic activity from cultures of Aureococcus anophagefferens—a causative species of brown tides in the north-western Bohai Sea, China

Cited by 18 publications

References 45 publications

Phytoplankton community structure in the Western Subarctic Gyre of the Pacific Ocean during summer determined by a combined approach of HPLC-pigment CHEMTAX and metabarcoding sequencing

Phytoplankton community structure in the Western Subarctic Gyre of the Pacific Ocean during summer determined by a combined approach of HPLC-pigment CHEMTAX and metabarcoding sequencing

Laboratory Culture-Based Characterization of the Resting Stage Cells of the Brown-Tide-Causing Pelagophyte, Aureococcus anophagefferens

Diversity and community structure of eukaryotic microalgae in surface sediments in the central Bohai Sea, China, based on a metabarcoding approach

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