2014
DOI: 10.1371/journal.pone.0085944
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Heparanase Interacts with Resistin and Augments Its Activity

Abstract: In an attempt to isolate a heparanase receptor, postulated to mediate non-enzymatic functions of the heparanase protein, we utilized human urine collected from healthy volunteers. Affinity chromatography of this rich protein source on immobilized heparanase revealed resistin as a heparanase binding protein. Co-immunoprecipitation and ELISA further confirmed the interaction between heparanase and resistin. Importantly, we found that heparanase potentiates the bioactivity of resistin in its standard bioassay in … Show more

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Cited by 11 publications
(9 citation statements)
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“…Furthermore, it is interesting to note that resistin has recently been identified as a new interacting partner for HPSE. Resistin has been shown to bind and signal through TLR4, leading to an increase in the pro-inflammatory protein MCP-1 [62] , [63] . How the interaction of HPSE and resistin regulates pro-inflammatory cytokine production is clearly of significant interest.…”
Section: Discussionmentioning
confidence: 99%
“…Furthermore, it is interesting to note that resistin has recently been identified as a new interacting partner for HPSE. Resistin has been shown to bind and signal through TLR4, leading to an increase in the pro-inflammatory protein MCP-1 [62] , [63] . How the interaction of HPSE and resistin regulates pro-inflammatory cytokine production is clearly of significant interest.…”
Section: Discussionmentioning
confidence: 99%
“…Indeed, heparanase-mediated cytokine release is reduced when cells are treated with the NF-κB inhibitor BAY117082 (Blich et al, 2013). Furthermore, it is interesting to note that resistin has been identified as a new interacting partner for heparanase (Novick et al, 2014). Resistin has been shown to bind and signal through TLR4, leading to an increase in the pro-inflammatory protein MCP-1 (Kopp et al, 2009).…”
Section: Heparanase In Acute and Chronic Inflammationmentioning
confidence: 99%
“…In humans, resistin circulates in trimeric and oligomeric forms of which the oligomeric form appears to have a more potent effect on the stimulation of proinflammatory cytokines [ 6 ]. Interestingly, the bioactivity of resistin is augmented by a physical interaction between resistin and heparanase [ 7 ].…”
Section: Resistin In Rodents and Humansmentioning
confidence: 99%