Heparin/DNA aptamer co-assembled multifunctional catecholamine coating for EPC capture and improved hemocompatibility of vascular devices

Deng, Jinchuan; Yuan, Shuheng; Li, Xin; Wang, Kebing; Xie, Lizhi; Li, Na; Wang, Jin; Huang, Nan

doi:10.1016/j.msec.2017.05.057

Cited by 23 publications

(8 citation statements)

References 45 publications

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“…Recently, Schulz et al showed that poly(ether imide) films functionalized with an aptamer-cRGD peptide improved the initial adherence and shear resistance of EC compared to unmodified films in vitro [ 85 ]. Another study investigated stents coated with heparin and aptamers regarding their potential to capture EPC and found that this modification effectively trapped arrested cells under dynamic conditions and regulated their distribution [ 86 ].…”

Section: Strategies To Generate or Mimic Endothelial Basement Membranesmentioning

confidence: 99%

Designing Cardiovascular Implants Taking in View the Endothelial Basement Membrane

Lau

Gossen

Lendlein

2021

IJMS

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Insufficient endothelialization of cardiovascular grafts is a major hurdle in vascular surgery and regenerative medicine, bearing a risk for early graft thrombosis. Neither of the numerous strategies pursued to solve these problems were conclusive. Endothelialization is regulated by the endothelial basement membrane (EBM), a highly specialized part of the vascular extracellular matrix. Thus, a detailed understanding of the structure–function interrelations of the EBM components is fundamental for designing biomimetic materials aiming to mimic EBM functions. In this review, a detailed description of the structure and functions of the EBM are provided, including the luminal and abluminal interactions with adjacent cell types, such as vascular smooth muscle cells. Moreover, in vivo as well as in vitro strategies to build or renew EBM are summarized and critically discussed. The spectrum of methods includes vessel decellularization and implant biofunctionalization strategies as well as tissue engineering-based approaches and bioprinting. Finally, the limitations of these methods are highlighted, and future directions are suggested to help improve future design strategies for EBM-inspired materials in the cardiovascular field.

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Section: Strategies To Generate or Mimic Endothelial Basement Membranesmentioning

confidence: 99%

Designing Cardiovascular Implants Taking in View the Endothelial Basement Membrane

Lau

Gossen

Lendlein

2021

IJMS

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“…The much smaller size of scFv fragments (~27 kDa vs. 150 kDa for IgGs) also enables their production using cheaper and faster bacterial expression systems while the absence of the antibody constant domains, including immune effector motifs, reduces nonspecific interactions with immune and other cells in vivo . While aptamers offer another alternative to effect cell binding due to their specificity, they have been hampered by their susceptibility to nuclease degradation leading to shorter half‐life and, for the present at least, less well‐developed routes to market compared to antibody molecules . EC‐specific proteins or peptides offer a further option in terms of cell binding and surface coating.…”

Section: Discussionmentioning

confidence: 99%

Functionalization with a VEGFR2‐binding antibody fragment leads to enhanced endothelialization of a cardiovascular stent in vitro and in vivo

et al. 2019

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Rapid endothelialization of cardiovascular stents is critical to prevent major clinical complications such as restenosis. Reconstruction of the native endothelium on the stent surface can be achieved by the capture of endothelial progenitor cells (EPCs) or neighboring endothelial cells (ECs) in vivo. In this study, stainless steel cardiovascular stents were functionalized with recombinant scFv antibody fragments specific for vascular endothelial growth factor receptor‐2 (VEGFR2) that is expressed on EPCs and ECs. Anti‐VEGFR2 scFvs were expressed in glycosylated form in Escherichia coli and covalently attached to amine‐functionalized, titania‐coated steel disks and stents. ScFv‐coated surfaces exhibited no detectable cytotoxicity to human ECs or erythrocytes in vitro and bound 15 times more VEGFR2‐positive human umbilical vein ECs than controls after as little as 3 min. Porcine coronary arteries were successfully stented with scFv‐coated stents with no adverse clinical events after 30 days. Endovascular imaging and histology revealed coverage of the anti‐VEGFR2 scFv‐coated stent with a cell layer after 5 days and the presence of a neointima layer with a minimum thickness of 80 μm after 30 days. Biofunctionalization of cardiovascular stents with endothelial cell‐capturing antibody fragments in this manner offers promise in accelerating stent endothelialization in vivo. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:213–224, 2020.

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“…After that, the samples were fixated with 2.5 wt% glutaraldehyde solution overnight. For the whole-blood test [ 51 ], the same volume (0.5 ml) of fresh human whole-blood was added onto each well of a 24-well plate containing samples. After 30 min incubation at 37°C, the samples were cleaned with PBS and fixed by 2.5% glutaraldehyde overnight.…”

Section: Methodsmentioning

confidence: 99%

Preparation of phospholipid-based polycarbonate urethanes for potential applications of blood-contacting implants

Cai

et al. 2020

Regenerative Biomaterials

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Polyurethanes are widely used in interventional devices due to the excellent physicochemical property. However, non-specific adhesion and severe inflammatory response of ordinary polyurethanes may lead to severe complications of intravenous devices. Herein, a novel phospholipid-based polycarbonate urethanes (PCUs) were developed via two-step solution polymerization by direct synthesis based on functional raw materials. Furthermore, PCUs were coated on biomedical metal sheets to construct biomimetic anti-fouling surface. The results of stress–strain curves exhibited excellent tensile properties of PCUs films. Differential scanning calorimetry results indicated that the microphase separation of such PCUs polymers could be well regulated by adjusting the formulation of chain extender, leading to different biological response. In vitro blood compatibility tests including bovine serum albumin adsorption, fibrinogen adsorption and denaturation, platelet adhesion and whole-blood experiment showed superior performance in inhibition non-specific adhesion of PCUs samples. Endothelial cells and smooth muscle cells culture tests further revealed a good anti-cell adhesion ability. Finally, animal experiments including ex vivo blood circulation and subcutaneous inflammation animal experiments indicated a strong ability in anti-thrombosis and histocompatibility. These results high light the strong anti-adhesion property of phospholipid-based PCUs films, which may be applied to the blood-contacting implants such as intravenous catheter or antithrombotic surface in the future.

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Heparin/DNA aptamer co-assembled multifunctional catecholamine coating for EPC capture and improved hemocompatibility of vascular devices

Cited by 23 publications

References 45 publications

Designing Cardiovascular Implants Taking in View the Endothelial Basement Membrane

Designing Cardiovascular Implants Taking in View the Endothelial Basement Membrane

Functionalization with a VEGFR2‐binding antibody fragment leads to enhanced endothelialization of a cardiovascular stent in vitro and in vivo

Preparation of phospholipid-based polycarbonate urethanes for potential applications of blood-contacting implants

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