Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary n6/n3 Ratios and DHA/EPA Supplementation in Streptozotocin Treated Rats

Starčević, Kristina; Filipović, Natalija; Galán, Asier; Micek, Vedran; Kurilj, Andrea Gudan; Mašek, Tomislav

doi:10.1002/mnfr.201701007

Cited by 17 publications

(12 citation statements)

References 45 publications

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“…This main oxidative mechanism is responsible for the creation of highly reactive derivatives, often damaging other membrane components, proteins, and nucleic acids [9,31]. Consistent with our data, lipid peroxidation has been significantly modified by PL supplementation, such as DHA/eicosapentaenoic acid (EPA), in streptozotocin-treated rats [32]. Challenging hippocampal cells with GPE significantly improved membrane fluidity, which is pivotal for proper membrane function and cell viability [7].…”

Section: Discussionsupporting

confidence: 76%

The Nootropic Drug Α-Glyceryl-Phosphoryl-Ethanolamine Exerts Neuroprotective Effects in Human Hippocampal Cells

Daniele

Mangano

Durando

et al. 2020

IJMS

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Brain aging involves changes in the lipid membrane composition that lead to a decrease in membrane excitability and neurotransmitter release. These membrane modifications have been identified as contributing factors in age-related memory decline. In this sense, precursors of phospholipids (PLs) can restore the physiological composition of cellular membranes and produce valuable therapeutic effects in brain aging. Among promising drugs, alpha-glycerylphosphorylethanolamine (GPE) has demonstrated protective effects in amyloid-injured astrocytes and in an aging model of human neural stem cells. However, the compound properties on mature neuronal cells remain unexplored. Herein, GPE was tested in human hippocampal neurons, which are involved in learning and memory, and characterized by a functional cholinergic transmission, thus representing a valuable cellular model to explore the beneficial properties of GPE. GPE induced the release of the main membrane phospholipids and of the acetylcholine neurotransmitter. Moreover, the compound reduced lipid peroxidation and enhanced membrane fluidity of human brain cells. GPE counteracted the DNA damage and viability decrease observed in in vitro aged neurons. Among GPE treatment effects, the autophagy was found positively upregulated. Overall, these results confirm the beneficial effects of GPE treatment and suggest the compound as a promising drug to preserve hippocampal neurons and virtually memory performances.

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Section: Discussionsupporting

confidence: 76%

The Nootropic Drug Α-Glyceryl-Phosphoryl-Ethanolamine Exerts Neuroprotective Effects in Human Hippocampal Cells

Daniele

Mangano

Durando

et al. 2020

IJMS

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“…The duration of the intervention lasted for 44 days: 14 days before induction of the DM1 and then 30 days after DM1 induction. In our previous works we proved that the same intervention resulted in substantial changes in total fatty acid composition of different tissues, as well as profound changes in fatty acid composition of phospholipids [27,28].…”

Section: Experimental Designmentioning

confidence: 74%

“…In the mouse brain, EPG contain more than 30 percent of n6 PUFAs, being also larger than the content of n3 (n3/n6 ratio = 0.77) [65]. During DM, a dramatic disturbance of the central and peripheral nervous system fatty acid metabolism and composition occurs [4,[66][67][68][69], but dietary fatty acid supplementation could prevent these disturbances [27]. Larger needs for myelin composition preservation could be an explanation for the more persistent effect of n6, in comparison to n3 PUFAs, in the prevention of myelinated sensory fibers disturbance that we found in the hearts of diabetic rats during the present study.…”

Section: Discussionmentioning

confidence: 99%

“…A total of 20 male Wistar rats were grown in standard polycarbonate cages in controlled environmental conditions (temperature at 22 ± 1 • C with a 12 h light/dark cycle, with free access to food and water). The standard laboratory diet (containing 20% crude protein, 5% crude fat, and 5% crude fiber) was adjusted with different ratios of n-6/n-3 using different oil blends, consisting of sunflower, fish, and linseed oil, as was published in detail previously [27]. The rats were randomly divided into 4 groups and were given different n6/n3 ratios, according to diet protocol in the study.…”

Section: Experimental Designmentioning

confidence: 99%

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Effects of Different n6/n3 PUFAs Dietary Ratio on Cardiac Diabetic Neuropathy

Urlić

Urlic

et al. 2020

Nutrients

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We studied the influence of experimentally induced DM1, in combination with different dietary n6:n3 polyunsaturated fatty acid (PUFA) ratios on different types of nerve fibers in rat myocardium, in order to reveal whether protective/unfavorable effects of different PUFAs on myocardial function in diabetic patients could be a (partial) repercussion of their effect on the changes in cardiac innervation. The control group (c) and diabetic group (stz) were fed with an n6/n3 ratio of ≈7; the diet of the stz+n6 group had an n6/n3 ratio ≈60, while the diet for the stz+DHA group contained 2.5% of fish oil (containing 16% eicosapentaenoic acid—EPA and 19% docosahexaenoic acid—DHA), n6/n3 ratio of ≈1. DM1 was induced by i.p. injection of streptozotocin (55 mg/kg) and rats were euthanized 30 days after induction. Immunohistochemistry was used for the detection and quantification of different types of neuronal fibers in the cardiac septum. We found changes in cardiac innervations characteristics for the initial phase of experimental DM1, which manifested as an increase in total number and area density of all neuronal fibers, measured by Pgp9.5 immunoreactivity. By detailed analysis, we found that this increase consisted mostly of heavy myelinated NF200 immunoreactive fibers and TH immunoreactive sympathetic fibers, while the density of ChAT immunoreactive parasympathetic fibers decreased. In the deep (middle) part of the myocardium, where rare fibers (of all studied types) were found, significant differences were not found. Surprisingly, we found a more consistent protective effect of n6 PUFAs, in comparison to n3 PUFAs supplementation. These results may provide a better understanding of the potential impacts of different PUFA ratios in the diet of diabetic patients on cardiac innervation and genesis and outcome of diabetic autonomic cardiomyopathy.

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“…The animals were assigned into three experimental groups, fed with different diets: the control group (CON, n6/n3 ratio: ≈7), the diabetic group that received a n3 enriched diet (STZ-N3, n6/n3 ratio: ≈ 1) and the diabetic group that received a n6 enriched diet (STZ-N6, n6/n3 ratio: ≈60). The rats were fed ad libitum with standard rodent feed whose composition has previously been described (STARČEVIĆ et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Corticosterone values and blood biochemistry in a model of streptozotocin-induced diabetes: the influence of dietary n6/n3 ratio

et al. 2019

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A. DOVČ, S. ŽUŽUL, M. BENIĆ, T. MAŠEK, K. STARČEVIĆ: Corticosterone values and blood biochemistry in a model of streptozotocin-induced diabetes: the influence of dietary n6/n3 ratio. Vet. arhiv 89, 885-894, 2019. ABSTRACTThe study was carried out to investigate the influence of dietary n6/n3 ratio in streptozotocin (STZ) induced diabetes on serum biochemistry and corticosterone values in blood in 15 Wistar male rats assigned into three experimental groups, two diabetic and a control group (STZ-N3, STZ-N6, CON) fed with different diets (n6/n3 ratio: ≈ 1, n6/n3 ratio: ≈60, CON n6/n3 ratio: ≈7). Significantly higher values of alkaline phosphatase (AP) and beta-hydroxybutyrate (BHB) and lower levels for triglyceride and albumin were noticed in both STZ treated groups compared to the control. The values for blood urea nitrogen (BUN) were increased in only the STZ-N3 group compared to the control (P<0.05). The values for the total bilirubin (P<0.05) and alanine aminotransferase (ALT) (P<0.05) were higher in only the STZ-N6 group compared to the control. In addition, Vet. arhiv 89 (6), 885-894, 2019 G. Gregurić Gračner et al.: Corticosterone values and blood biochemistry in a model of streptozotocin-induced diabetes: the influence of dietary n6/n3 ratio the STZ-N3 group had lower albumin values compared to the STZ-N6 group (P < 0.001). Corticosterone values did not significantly differ among all three experimental groups indicating similar levels of stress. In conclusion, the n6/n3 ratio significantly influences blood biochemical parameters in STZ-treated rats. Nevertheless, neither the n6/n3 ratio nor blood sampling and handling influences corticosterone values, which proved the validity of the STZ-induced rodent model of diabetes mellitus in the experimental conditions described.

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Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary n6/n3 Ratios and DHA/EPA Supplementation in Streptozotocin Treated Rats

Abstract: Presented data suggest that the n6/n3 ratio could significantly influence lipogenesis, lipid peroxidation, and β-oxidation in STZ-induced diabetes, which could have clinical significance.

Cited by 17 publications

References 45 publications

The Nootropic Drug Α-Glyceryl-Phosphoryl-Ethanolamine Exerts Neuroprotective Effects in Human Hippocampal Cells

The Nootropic Drug Α-Glyceryl-Phosphoryl-Ethanolamine Exerts Neuroprotective Effects in Human Hippocampal Cells

Effects of Different n6/n3 PUFAs Dietary Ratio on Cardiac Diabetic Neuropathy

Corticosterone values and blood biochemistry in a model of streptozotocin-induced diabetes: the influence of dietary n6/n3 ratio

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