Hepatic protein synthesis and morphological parameters in blood of rats under oxidative stress and action of feed additive Butaselmevit-plus

Ivankiv, M.; Kachmar, N.; Mazurak, O.; Martyshuk, T. V.

doi:10.15421/2019_800

Cited by 12 publications

(11 citation statements)

References 8 publications

(9 reference statements)

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“…Currently, there is a large amount of reports about important role of the system of antioxidative protection in animals during development of toxicoses caused by heavy metals (Gutyj et al, 2016;Ivankiv et al, 2019;Vasylyev et al, 2021). Therefore, it was important to study the impact of the feed additive Metisevit Plus on enzymic activity of the antioxidant system and glutathione link of the organisms of bulls in the conditions of technogenic pressure from lead and cadmium.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors

Slobodian

Gutyj

Darmohray

et al. 2021

Regul. Mech. Biosyst.

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Prolonged ingress of heavy metals into the organisms of animals, even in low concentrations, causes a number of toxic impacts, affecting various organs and systems. The objective of this study was to research parameters of enzymatic and non-enzymatic links of the system of antioxidant protection of the organism of young cattle in the condition of lead-cadmium load and the effect of Metisevit Plus feed additive. Studies were conducted on the basis of the Private Agricultural Company “Ukraina”, located in Dubrovytsia district of Rivne Oblast, using 12 six-month old bulls of Ukrainian Black Pied Dairy breed divided into two groups, each consisting of 6 animals. Bulls of the control group received the standard diet. The bulls of the experimental group were fed with Metisevit Plus feed additive in the dose of 0.5 g/kg of feed. The contents of lead and cadmium in feed at the enterprise were determined to be high. According to the conducted studies, it was determined that in conditions of cadmium and lead load, there is a decrease in activity of glutathione link of the system of antioxidant protection of the organism of bulls. Feeding the bulls of the experimental group with Metisevit Plus feed additive for 30 days led to increase in the levels of non-enzymatic and enzymatic activities of the glutathione system. Significant increase in the level of reduced glutathione was recorded on days 30 and 40 of the experiment. Consumption of Metisevit Plus by the animals of the experimental group promoted increase in the activity of catalase and superoxide dismutase starting on the 20th day of the experiment. On days 30 and 40 of the experiment, the activity of the studied enzymes ranged within the physiological values. Therefore, the studies conducted on the bulls indicate that in the conditions of cadmium and lead load the feed additive Metisevit Plus fed to the bulls activated the system of antioxidant protection by increasing the levels of enzymatic and non-enzymatic links. When feeding bulls of the experimental group with Metisevit Plus feed additive, we determined significant decreases in diene conjugates and TBA-active products in their blood starting already on the 10th day of the experiment. On days 20 and 30 of the experiment, the level of diene conjugates in the blood of animals of the experimental group decreased by 14.5% and 24.0%. Efficiency of the feed additive may be explained by the effect of its constituents on the main pathogenic links of the pathological process caused by cadmium and lead. As indicated in the studies, liquidation of the syndrome of endogenous intoxication restores the functional condition of cellular membranes of the liver, protein-synthesizing function of the liver, increases the antioxidant status of the organisms of bulls. The studies we conducted confirm the expedience of using feed additive Metisevit Plus to prevent lead-cadmium toxicosis.

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Section: Discussionmentioning

confidence: 99%

Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors

Slobodian

Gutyj

Darmohray

et al. 2021

Regul. Mech. Biosyst.

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“…Free glutathione with the participation of NADPH under the influence of glutathione peroxidase interacts with free radicals and inactivates their toxic effects due to oxidation of glutathione (Khariv et al, 2016;. Oxidized glutathione is reduced under the influence of glutathione reductase, which is induced under the conditions of oxidative stress (Lavryshyn et al, 2016;Martyshuk, 2016;Ivankiv et al, 2019).…”

Section: Figmentioning

confidence: 99%

“…It is known that piglets are able to digest only the protein and fat of sow's milk, which is their main food until the 20th day (Martyshuk et al, 2019;Han et al, 2019;Leskiv et al, 2021). Early weaning (days 18-31) of piglets allows the sow to be used more intensively .…”

Section: Introductionmentioning

confidence: 99%

System of antioxidant protection of the body of piglets under the action of feed additive “Butaselmevit-plus”

Martyshuk

Gutyj

Khalak³

2021

Ukr. Jour. of Vet. and Agr. Sci.

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The aim of the study was to investigate the effect of the feed additive “Butaselmevit-plus” on the state of the antioxidant defense system of piglets during weaning. The experiments were conducted on the basis of the LTD “KOSHET” Mukachevo district of Zakarpattia region. Two groups of piglets were formed – control and experimental, in the amount of 10 individuals in each group, selected on the principle of analogs – age, breed and body weight. Animals were fed according to the norms for this age of pigs. On the 28th day of life, the piglets were weaned from the sow and regrouped from different nests for further maintenance during fattening and rearing with a change in the structure of the diet, which served as technological stress for the animal's body. Piglets of the experimental group, from 21 to 40 days of age, were additionally fed the feed additive “Butaselmevit-plus” at a dose of 100 mg/kg body weight per day. The material for the study was blood, which was taken in the morning before feeding the animals by puncture of the cranial vena cava for 20 days of life (the period before weaning), for 25 days of life (the period before weaning), for 30 days of life (2 days after weaning), for 35 days of life (7 days after weaning), 40 days of life (12 days after weaning). It is proved that feeding piglets feed additive “Butaselmevit-plus” enhanced the antioxidant status of piglets after weaning. It was found that under conditions of technological stress, the use of feed additive “Butaselmevit-plus” in the amount of 100 mg/kg body weight per day improves the antioxidant defense of the body, as evidenced by an increase in blood 35-day-old piglets superoxide dismutase activity by 26.7 % (P < 0.001), catalase – by 39.5 % (P < 0.001), glutathione peroxidase – by 51.7 % (P < 0.05), glutathione reductase – by 52.9 % (P < 0.05), the content of reduced glutathione – at 58.3 % (P < 0.001). These results confirm the effectiveness of the use of milk thistle, methionine, selenium, and vitamins A, E, and D3 in the feed additive “Butaselmevit-plus” to piglets in the period before and after weaning to activate the protective systems of their body.

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“…Підвищення концентрації первинних та кінцевих продуктів пероксидації ліпідів та активності каталази крові свідчить про початок розвитку оксидативного стресу (Ivankiv et al, 2019;Slobodian et al, 2019;Martyshchuk & Gutyj, 2019) та стимуляцію пероксидних процесів у риб дослідної групи та порушення цілісности мембранних структур клітин.…”

Section: таблицяunclassified

Monitoring of productive and biochemical parameters of young Clarias gariepinus during feeding of Skretting and Roycher AQUA feeds in experimental conditions

et al. 2021

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The article presents the results of monitoring the productive and biochemical indicators of the growth rate of young clary catfish using dry extruded complete feed for fish, which have proven to be best adapted for use at all stages of growing clary catfish, namely: Skretting and Roycher AQUA in experimental conditions. The research was conducted in the educational and experimental laboratories of Bila Tserkva National Agrarian University from 2019 to 2021. For the experiment, we used the young of the African marble clary catfish (Clarias gariepinus), purchased from one of the fish farms in the Odesa region. Clary catfish fry was kept in four rectangular plastic containers for 90 liters of freshwater. The tanks were two-thirds full (60 liters). The water temperature was maintained at 26 °C. For the experiment, 120 fries were used, which were kept in four plastic tanks by the method of analogs of 30 specimens each. Given that the fry differed in weight (for experiment 1 we selected fry with higher weight, and for experiment 2 – with lower weight), they were divided on the principle of analogs into two experimental and two control groups. Based on the results of monitoring in Experiment 1, fish observations, and mathematical calculation, we found that the feed ratio when fed Skretting and Roycher AQUA starter feed is 0.74 and 0.99 %, respectively. Based on the results of monitoring, fish observations, and mathematical calculation, it was found that the feed ratio in experiment 2 when feeding starter feed Skretting and Roycher AQUA is 0.75 and 1.54 %, respectively. The high feed rate for the use of Roycher AQUA can be explained by overuse due to the grinding of the granules. Growing aquaculture facilities in an artificially formed system – production, allows you to get environmentally friendly fish products all year round. This is a very important criterion in modern environmental conditions.

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Hepatic protein synthesis and morphological parameters in blood of rats under oxidative stress and action of feed additive Butaselmevit-plus

Cited by 12 publications

References 8 publications

Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors

Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors

System of antioxidant protection of the body of piglets under the action of feed additive “Butaselmevit-plus”

Monitoring of productive and biochemical parameters of young Clarias gariepinus during feeding of Skretting and Roycher AQUA feeds in experimental conditions

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