Hepatic stem cell niches

Kordes, Claus; Häussinger, Dieter

doi:10.1172/jci66027

Cited by 130 publications

(111 citation statements)

References 113 publications

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“…In line with this, stellate cells can originate from the bone marrow (BM) (10,11), where MSCs were initially discovered (12), and reside in the liver close to endothelial cells, as is the case for MSCs in other organs (13). Quiescent stellate cells are typically located between sinusoidal endothelial cells and hepatocytes in the space of Disse, which has characteristics of stem cell niches (14,15). Moreover, activated stellate cells can develop into hepatocyte-like cells in vitro (14,16), and fate-mapping experiments using Gfap and Acta2 promoters indicate that they contribute to liver regeneration in vivo (17)(18)(19).…”

Section: Introductionmentioning

confidence: 90%

Hepatic stellate cells contribute to progenitor cells and liver regeneration

Kordes¹,

Sawitza²,

Götze³

et al. 2014

J. Clin. Invest.

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147

112

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Section: Introductionmentioning

confidence: 90%

Hepatic stellate cells contribute to progenitor cells and liver regeneration

Kordes¹,

Sawitza²,

Götze³

et al. 2014

J. Clin. Invest.

Self Cite

147

112

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“…2,25 In cirrhotic human livers, cholangiocytes, identified by cytokeratin 19, show similar distribution of staining as CXCL12 in hepatic sections from the same patient ( Figure 1A). Similarly, in normal and bile duct Figure 2 Murine and human cholangiocytes do not express CXCL12.…”

Section: Detection Of Cxcl12 Staining In Becs With Cxcl12 Antibody CLmentioning

confidence: 84%

Biliary Epithelial Cells Are Not the Predominant Source of Hepatic CXCL12

Saiman

Sugiyama

Simchoni

et al. 2015

The American Journal of Pathology

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Hepatic expression levels of CXCL12, a chemokine important in inflammatory and stem cell recruitment, and its receptor, C-X-C chemokine receptor 4, are increased during all forms of liver injury. CXCL12 is expressed by both parenchymal and nonparenchymal hepatic cells, and on the basis of immunohistochemistry, biliary epithelial cells (BECs) are thought to be a predominant source of hepatic CXCL12, thereby promoting periportal recruitment of C-X-C chemokine receptor 4eexpressing lymphocytes. Our study aims to show that BECs may, in fact, not be the predominant source of hepatic CXCL12. We measured CXCL12 secretion and expression from human and murine BECs using enzyme-linked immunosorbent assay and Western blot analysis from cell culture supernatants and whole cell lysates, respectively, whereas CXCL12 expression in murine livers was analyzed in a Cxcl12-Gfp reporter mouse. Cell culture supernatants and whole cell lysates from BECs failed to demonstrate their expression of CXCL12. Furthermore, we confirmed these results with a Cxcl12-Gfp reporter mouse in which green fluorescent protein expression is notably absent from BECs. Interestingly, on the basis of green fluorescent protein expression, we demonstrate a population of CXCL12-expressing cells within the portal tract that are distinct, yet intimately associated with BECs. These findings indicate that BECs are not a predominant source of CXCL12. (Am J Pathol 2015 http://dx

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“…Under the control of the stem cell niche, stem cells are maintained in a balance between self-renewal and differentiation in the G0 phase (Kordes et al, 2013;Pérez Mdel et al, 2013). Stem cell niches provide a microenvironment for stem cells to control proliferation and inhibit differentiation (Hanoun et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Growth, Clonability, and Radiation Resistance of Esophageal Carcinoma-derived Stem-like Cells

Liu²,

Yang³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Objective: To separate/enrich tumor stem-like cells from the human esophageal carcinoma cell line OE-19 by using serum-free suspension culture and to identify their biological characteristics and radiation resistance. Methods: OE-19 cells were cultivated using adherent and suspension culture methods. The tumor stem-like phenotype of CD44 expression was detected using flow cytometry. We examined growth characteristics, cloning capacity in soft agar, and radiation resistance of 2 groups of cells. Results: Suspended cells in serum-free medium formed spheres that were enriched for CD44 expression. CD44 was expressed in 62.5% of suspended cells, but only in 11.7% of adherent cells. The suspended cells had greater capacity for proliferation and colony formation in soft agar than the adherent cells. When the suspended and adherent cells were irradiated at 5 Gy, 10 Gy, or 15 Gy, the proportion of CD44+ suspended cells strongly and weakly positive for CD44 was 77.8%, 66.5%, 57.5%; and 21.7%, 31.6%, 41.4%, respectively. In contrast, the proportion of CD44+ adherent cells strongly positive for CD44 was 18.9%, 14.%, and 9.95%, respectively. When the irradiation dose was increased to 30 Gy, the survival of the suspended and adherent cells was significantly reduced, and viable CD44+ cells were not detected. Conclusion: Suspended cell spheres generated from OE-19 esophageal carcinoma cells in serum-free stem medium are enriched in tumor stem-like cells. CD44 may be a marker for these cells.

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Hepatic stem cell niches

Cited by 130 publications

References 113 publications

Hepatic stellate cells contribute to progenitor cells and liver regeneration

Hepatic stellate cells contribute to progenitor cells and liver regeneration

Biliary Epithelial Cells Are Not the Predominant Source of Hepatic CXCL12

Growth, Clonability, and Radiation Resistance of Esophageal Carcinoma-derived Stem-like Cells

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