Hepatitis B and Hepatitis C Infection Biomarkers and TP53 Mutations in Hepatocellular Carcinomas from Colombia

Navas, María Cristina; Suarez, Iris; Carreño, Andrea Milena Guatibonza; Uribe, Diego; Rios, Wilson Alfredo; Cortés-Mancera, Fabián; Martel, Ghyslaine; Vieco, Beatriz; Lozano, Diana C; Jimenez, Carlos; Gouas, Doriane; Osorio, Germán; Hoyos, Sergio; Restrepo, Juan Carlos; Correa, Gonzalo; Jaramillo, Sergio; López, Rocío; Bravo, Luis Eduardo; Arbeláez, María Patricia; Scoazec, Jean‐Yves; Abedi-Ardekani, Behnoush; Santella, Regina M.; Chemin, Isabelle; Hainaut, Pierre

doi:10.1155/2011/582945

Cited by 13 publications

(13 citation statements)

References 30 publications

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“…Further, specific epigenetic processes may be affected by aflatoxins, especially during a prolonged period of exposure, promoting tumorigenesis, angiogenesis, invasion and metastasis to other organs in cases of hepatocellular carcinoma (Bbosa et al 2013). In a study conducted in Colombia, 10.5% of 202 HCC cases had the characteristic aflatoxin-induced mutation of codon 249 of the p53 tumour suppressor gene (Navas et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Determination of aflatoxin M₁in breast milk as a biomarker of maternal and infant exposure in Colombia

Diaz

Sánchez

2015

Food Additives & Contaminants: Part A

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Chronic exposure to aflatoxins, and especially to aflatoxin B1 (AFB1), causes hepatocellular carcinoma with prevalence 16-32 times higher in developing compared with developed countries. Aflatoxin M1 (AFM1) is a monohydroxylated metabolite from AFB1 that is secreted in milk and which can be used as a biomarker of AFB1 exposure. This study aimed to determine AFM1 levels in human breast milk using immunoaffinity column clean-up with HPLC and fluorescence detection. Breast milk samples were obtained from 50 nursing mothers. Volunteers filled in a questionnaire giving their consent to analyse their samples as well as details of their socioeconomic, demographic and clinical data. The possible dietary sources of aflatoxins were assessed using a food frequency questionnaire. A total of 90% of the samples tested positive for AFM1, with a mean of 5.2 ng l(-1) and a range of 0.9-18.5 ng l(-1). The study demonstrated a high frequency of exposure of mothers and neonates to AFB1 and AFM1 in Colombia, and it points out the need to regulate and monitor continuously the presence of aflatoxins in human foods. Further research is needed in order to determine the presence of other mycotoxins in foods and in human samples as well as to devise protection strategies in a country where mycotoxins in human foods are commonly found.

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Section: Introductionmentioning

confidence: 99%

Determination of aflatoxin M₁in breast milk as a biomarker of maternal and infant exposure in Colombia

Diaz

Sánchez

2015

Food Additives & Contaminants: Part A

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“…They include TNF- α , TGF β , PGF, and other factors contributing towards the fibrogenesis [ 5 , 6 ]. Among these, TNF- α is the most important cytokine in host immune response to viral infection [ 7 , 8 ]. TNF- α is a pleiotropic cytokine, located 850 kb telomeric of class II HLA-DR locus of the short arm of chromosome 6, which induces cellular responses such as proliferation, production of inflammatory mediators, and cell death [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

The TNF-α -308 Promoter Gene Polymorphism and Chronic HBV Infection

Tayebi

Ashraf

2012

Hepatitis Research and Treatment

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Background and Aims. TNF-α -308 allele promoter polymorphism has been known to be a potential prognostic factor in patients with chronic HBV infection. We tried to determine how TNF-α -308 allele promoter polymorphism would affect the prognosis in patients with chronic HBV infection. Methods. We searched MEDLINE, EMBASE, and reference lists of relevant review articles related to the association between “TNF-α G-308A promoter polymorphism” with “chronic HBV infection”. We only focused on searching -308 locus in published studies. We reviewed 21 original articles about TNF-α -308 allele polymorphism and its effect on prognosis in patients with chronic HBV infection and discussed the results. Results. conflicting results were observed. The results were divided into 3 groups including neutral, negative, and positive associations between TNF-α -308 allele polymorphism and prognosis in patients with chronic HBV infection. We summarized the primary data as a table. Conclusions. Authors concluded that although there is an upward trend in evidence to claim that there is a positive relation between TNF-α G-308A promoter polymorphisms and resolution of chronic HBV infection, due to many biases and limitations observed in reviewed studies, an organized well-designed study is needed for clarifying the real association.

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“…(20) In Colombia, the R249S mutation was found in only 4 of the 38 HCC cases (11%), with twenty-five of the cases being positive for HBV infection. (22) In Mexico, 18% of HCC samples (3/16) had the AFB 1 -signature mutation, with only 3 cases being positive for HBV. 21As maize is commonly consumed in a number of Latin American countries, exposure to AFB 1 is likely.…”

Section: Discussionmentioning

confidence: 99%

“…(12)(13)(14)(15)(16)(17)(18)(19) In the Americas, the R249S mutation prevalence reportedly ranges between 10.5% and 28%. (20)(21)(22) In the U.S., a recent study reported that 7% of the HCCs among Hispanic patients in southern Texas had the R249S mutation. (23) No studies have been previously conducted in northern Central America, a region characterized by high AFB 1 exposure, but low prevalence of chronic HBV infection.…”

Section: Introductionmentioning

confidence: 99%

Analysis of TP53 gene mutations demonstrates that aflatoxin is a risk factor for hepatocellular carcinoma in Guatemala

Álvarez¹,

Ortiz

Bendfeldt-Avila

et al. 2019

Preprint

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Background Guatemala has the highest incidence of hepatocellular carcinoma (HCC), the dominant type of liver cancer, in the Western Hemisphere. The major risk factors in Guatemala are not well-characterized, but the prevalence of hepatitis B (HBV) and hepatitis C virus (HCV) appear to be low, while the prevalence of aflatoxin (AFB 1 ) exposure appears to be high. To examine whether AFB 1 could be a risk factor for HCC in Guatemala, this study examined the frequency of the AFB 1 -signature mutation in the TP53 gene (R249S) as well as other somatic mutations.Methods Ninety-one formalin-fixed, paraffin-embedded (FFPE) HCC tissues were obtained from three hospitals in Guatemala City. An additional, eighteen tumor tissues preserved in RNAlater were also obtained. Targeted sequencing of TP53 was successfully performed in 89 of the FFPE samples, and a panel of 245 genes were sequenced in the RNAlater samples.Results Overall, 47% of HCCs had a TP53 mutation. The AFB 1 -signature R249S mutation was present in 24%. Among the RNAlater samples, 44% had any TP53 mutation and 33% had the R249S mutation. Other somatic mutations were identified in known HCC driver genes such as ARID1A , ARID2 , and CTNNB1 .Conclusions The presence of the TP53 R249S mutation indicates that AFB 1 is a risk factor for HCC in Guatemala. The proportion of HBV positive tumors was low, suggesting that AFB 1 is associated with HCC in the absence of concomitant HBV infection. To decrease the risk of HCC in Guatemala, AFB 1 abatements efforts are warranted.

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Hepatitis B and Hepatitis C Infection Biomarkers and TP53 Mutations in Hepatocellular Carcinomas from Colombia

Cited by 13 publications

References 30 publications

Determination of aflatoxin M₁in breast milk as a biomarker of maternal and infant exposure in Colombia

Determination of aflatoxin M₁in breast milk as a biomarker of maternal and infant exposure in Colombia

The TNF-α -308 Promoter Gene Polymorphism and Chronic HBV Infection

Analysis of TP53 gene mutations demonstrates that aflatoxin is a risk factor for hepatocellular carcinoma in Guatemala

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Hepatitis B and Hepatitis C Infection Biomarkers and TP53 Mutations in Hepatocellular Carcinomas from Colombia

Cited by 13 publications

References 30 publications

Determination of aflatoxin M1in breast milk as a biomarker of maternal and infant exposure in Colombia

Determination of aflatoxin M1in breast milk as a biomarker of maternal and infant exposure in Colombia

The TNF-α -308 Promoter Gene Polymorphism and Chronic HBV Infection

Analysis of TP53 gene mutations demonstrates that aflatoxin is a risk factor for hepatocellular carcinoma in Guatemala

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Product

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Determination of aflatoxin M₁in breast milk as a biomarker of maternal and infant exposure in Colombia

Determination of aflatoxin M₁in breast milk as a biomarker of maternal and infant exposure in Colombia