Hepatitis B virus genotypes among chronic hepatitis B patients reporting at Korle-Bu teaching hospital, Accra, Ghana

Dongdem, Anthony Zunuo; Dzodzomenyo, Mawuli; Asmah, Richard Harry; Nyarko, Kofi Mensah; Nortey, Priscillia; Agyei, Adwoa; Adjei, David Nana; Kenu, Ernest; Adjei, Andrew A.

doi:10.11604/pamj.supp.2016.25.1.6170

Cited by 12 publications

(9 citation statements)

References 25 publications

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“…This has been attributed to mutations in the B-cell and T-cell epitopes in the S-gene of the HBV genome that may lead to breakthrough infections even among the vaccinated [ 55 ] as well as differences in the infecting genotype [ 56 ]. We did not observe a significant association between genotype A and age in conformity with the findings from other studies [ 57 , 58 ]. However, Muriel et al [ 56 ] observed a significantly increased risk of infection with genotype A among the persons aged between 29 and 41 years.…”

Section: Discussionsupporting

confidence: 92%

Disproportionate Distribution of HBV Genotypes A and D and the Recombinant Genotype D/E in the High and Low HBV Endemic Regions of Uganda: A Wake-Up Call for Regional Specific HBV Management

Kafeero

Ndagire

Ocama

et al. 2022

International Journal of Hepatology

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Background. Hepatitis B virus (HBV) is the leading cause of liver-related diseases. In Uganda, there is a regional disparity in the HBV burden. Our study was aimed at establishing the circulating genotypes in a low and a high endemic region to give plausible explanations for the differences in regional burden and guide the future management of the disease. Methods. A total of 200 HBsAg-seropositive subjects were recruited into the study by convenience sampling. The HBsAg Rapid Test Strip (Healgen Scientific Limited Liability Company, Houston, TX77047- USA) was used to screen for HBsAg while the Roche machine (Roche, Basel Switzerland/Abbot Technologies (USA)) was used to determine the viral load. The Chemistry Analyzer B120 (Mindray, China) was used for chemistry analysis. For HBV genotyping, total DNA was extracted from whole blood using the QIAamp® DNA extraction kit. Nested PCR amplification was performed using Platinum Taq DNA Polymerase (Invitrogen Corporation, USA) to amplify the 400 bp HBV polymerase gene. Purification of nested PCR products was performed using Purelink PCR product purification kit (Life Technologies, USA). Automated DNA sequencing was performed using BigDye Terminator v3.1 Cycle Sequencing Kit on 3130 Genetic Analyzer (Applied Biosystems, USA). The NCBI HBV genotyping tool (https://www.ncbi.nlm.nih.gov/projects/genotyping/formpage.cgi) was used for determination of genotype for each HBV sequence. Pearson’s chi-square, multinomial logistic regression, and Mann–Whitney U tests were used for the analysis. All the analyses were done using SPSS version 26.0 and MedCalc software version 19.1.3 at 95% CI. A p < 0.05 was considered statistically significant. Results. Majority of our study subjects were female (64.5%), youth (51.0%), and married (62.0%). Overall, genotype A was the most prevalent (46%). Genotype D and the recombinant genotype D/E were proportionately more distributed in the high endemic (38.2%) and low endemic (36.5%) regions, respectively. Genotype D was significantly more prevalent in the high endemic region and among the elderly ( p < 0.05 ). Genotype D was significantly associated with elevated viral load and direct bilirubin ( p < 0.05 ). The recombinant genotype D/E was significantly associated with elevated viral load ( p < 0.05 ). Similarly, genotype A was significantly associated with elevated AST and GGT, lowered viral load, and normal direct bilirubin levels ( p < 0.05 ). Conclusion. There is disproportionate distribution of genotypes A and D and the recombinant genotype D/E in the low and high endemic regions of Uganda. This probably could explain the differences in endemicity of HBV in our country signifying the need for regional specific HBV management and control strategies.

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Section: Discussionsupporting

confidence: 92%

Disproportionate Distribution of HBV Genotypes A and D and the Recombinant Genotype D/E in the High and Low HBV Endemic Regions of Uganda: A Wake-Up Call for Regional Specific HBV Management

Kafeero

Ndagire

Ocama

et al. 2022

International Journal of Hepatology

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“…Characterization of HBV genotypes allows clinicians to determine patients’ response to treatment and potential risks of complications[ 58 , 73 ]. Flink et al[ 74 ] have indeed reported that genotype A responds better to interferon alpha and pegIFNα than genotype D. Genotype recombination occurs in areas where multiple genotypes are in co-circulation, thus facilitating diversification between individuals within the general population.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of hepatitis viruses in West-African countries from 1996 to 2018

Assih

Ouattara

Diarra

et al. 2018

WJH

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The severity of hepatic pathology and the response to treatment depend on the hepatitis virus genotype in the infected host. The objective of this review was to determine the distribution of hepatitis virus genotypes in West African countries. A systematic review of the literature in PubMed, Google Scholar and Science Direct was performed to identify 52 relevant articles reporting hepatitis A, B, C, D, E and G viruses genotypes. Hepatitis B virus (HBV) genotype E with a prevalence of 90.6% (95%CI: 0.891-0.920) found in this review, is characterized by low genetic diversity. Hepatitis C virus (HCV) genotypes 1 and 2 represented 96.4% of HCV infections in West African countries, while hepatitis delta virus, hepatitis A virus, hepatitis G virus genotypes 1 and HEV genotype 3 were reported in some studies in Ghana and Nigeria. HBV genotype E is characterized by high prevalence, low genetic diversity and wide geographical distribution. Further studies on the clinical implications of HBV genotype E and HCV genotypes 1 and 2 are needed for the development of an effective treatment against this viral hepatitis in West African countries. Surveillance of the distribution of different genotypes is also needed to reduce recombination rates and prevent the emergence of more virulent viral strains.

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“…Hepatitis may be caused by viruses or by medicines or alcohol. Hepatitis has the following forms: Hepatitis A, B, C, D and E [28]: Hepatitis B: A virus commonly spread by sexual intercourse or blood transfusion or from mother to newborn at birth. Another way it spreads is by using a needle that was used by an infected person.…”

Section: Definition Of Hepatitismentioning

confidence: 99%

Infectious Hepatitis B Negatively Impacts Lifestyles of Affected Individuals in Ejura-Sekyedumasi District of Ghana

Tiimub¹,

Dzomeku²,

Tiimob³

et al. 2020

EASJPID

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Hepatitis B virus genotypes among chronic hepatitis B patients reporting at Korle-Bu teaching hospital, Accra, Ghana

Cited by 12 publications

References 25 publications

Disproportionate Distribution of HBV Genotypes A and D and the Recombinant Genotype D/E in the High and Low HBV Endemic Regions of Uganda: A Wake-Up Call for Regional Specific HBV Management

Disproportionate Distribution of HBV Genotypes A and D and the Recombinant Genotype D/E in the High and Low HBV Endemic Regions of Uganda: A Wake-Up Call for Regional Specific HBV Management

Genetic diversity of hepatitis viruses in West-African countries from 1996 to 2018

Infectious Hepatitis B Negatively Impacts Lifestyles of Affected Individuals in Ejura-Sekyedumasi District of Ghana

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