Hepatitis B virus X protein induces apoptosis in hepatoma cells through inhibiting Bcl-xL expression

Hepatitis B virus X protein promotes liver cell proliferation via a positive cascade loop involving arachidonic acid metabolism and p-ERK1/2

“…Our conclusions are consistent with previous results in which HBx induced proliferation in cultured cells [27] and another study in which HBx expression induced cell-cycle progression within the milieu of the regenerating liver [28]. The results of our study oppose the conclusions of other studies that showed that transient transfection of HBx induced apoptosis and that HBx inhibited hepatocyte regeneration [29][30][31]. The reasons behind this apparent discrepancy need to be further investigated.…”

Increased expression of nucleophosmin/B23 in hepatocellular carcinoma and correlation with clinicopathological parameters

“…Six hepatoma cell lines including Hep G2, Huh-7, PLC/PRF/5, SKHep-1, Chang, and Hep-3B were cultured as reported previously (Yun et al, 2003;Miao et al, 2006). Briefly, for immunocytofluorescence, the cultured cells were fixed for 10 min and rinsed with PBS.…”

“…The fluorescent signal was observed under fluorescent microscopy (Yun et al, 2003). For immunolabelling, lysates from the tissue samples were prepared as reported previously (Yun et al, 2003;Miao et al, 2006). One hundred micrograms of each lysate was separated by SDS -PAGE.…”

Signal transduction cascades and hepatitis B and C related hepatocellular carcinoma

“…97 The PLC/ PRF/5 cell line has previously been shown to express low levels of HBx, 98 while HepB3 cells do not express HBx. 99 In addition, co-transfecting Huh-7 cells (derived from an HBV-negative liver) with HBx and Wnt-1 caused a dosedependent increase in TCF-4-dependent transcriptional activity, and increased nuclear translocation of ␤-catenin. 97 Introduction of a Src dominant-negative mutant blocked nuclear translocation of ␤-catenin in these same cells.…”