Hepatitis E Virus in Rabbits, Virginia, USA

Cossaboom, Caitlin M.; Córdoba, Laura; Dryman, Barbara A.; Meng, Xiang‐Jin

doi:10.3201/eid1711.110428

Cited by 143 publications

(144 citation statements)

References 16 publications

Supporting

Mentioning

141

Contrasting

Unclassified

Order By: Relevance

“…[3][4][5][6] Genotype 3 infects the broadest spectrum of hosts (human, swine, wild boar, deer, rabbit, mongoose, etc.) and has the greatest genetic diversity, with 10 identified subgenotypes (3a to 3j).…”

Section: Introductionmentioning

confidence: 99%

High Prevalence of Hepatitis E in Humans and Pigs and Evidence of Genotype-3 Virus in Swine, Madagascar

Temmam¹,

Besnard²,

Andriamandimby³

et al. 2013

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Hepatitis E virus (HEV) causes an orofecal disease transmitted through poor hygiene environments, contaminated food (mainly pork products), or by contacts with infected animals. Very little data are currently available regarding the disease in the Southwestern Indian Ocean Islands. We report the first sero-and viro-survey for HEV in human and swine in Madagascar. A seroprevalence rate of 14.1% (60 of 427) was measured in slaughterhouse workers. Seroprevalence to HEV in pigs was estimated to 71.2% (178 of 250), strongly suggesting the existence of a zoonotic cycle. Three out of 250 pig livers (1.2%) tested HEV RNA-positive by quantitative polymerase chain reaction. Phylogenetic analyses based on 1-kb sequences of the ORF 2-3 identified these viruses as HEV genotype 3. Sequences clustered in a distinct Malagasy sub-clade, possibly representative of a new sub-genotype, for which the date of emergence was estimated around 1989. Further studies are needed to confirm other transmission routes of HEV to humans, especially through non-zoonotic cycles.

show abstract

Section: Introductionmentioning

confidence: 99%

High Prevalence of Hepatitis E in Humans and Pigs and Evidence of Genotype-3 Virus in Swine, Madagascar

Temmam¹,

Besnard²,

Andriamandimby³

et al. 2013

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…Genotypes 3 and 4 have been isolated from many species, including pigs, wild boars, deer, rats (Rattus spp. ), rabbits (Leporidae), mongooses (Herpestidae), and horses (Equus ferus caballus) (Meng et al 1997;Nakamura et al 2006;Saad et al 2007;Geng et al 2010;Johne et al 2010;Cossaboom et al 2011).…”

mentioning

confidence: 99%

High Prevalence of Hepatitis E Virus in Wild Boar (Sus scrofa) in Yamaguchi Prefecture, Japan

Hara

Terada

Yonemitsu

et al. 2014

Journal of Wildlife Diseases

View full text Add to dashboard Cite

ABSTRACT:Hepatitis E virus (HEV) causes a food-and water-borne disease in humans, and Japanese wild boar (Sus scrofa leucomystax) meat is one of the most important sources of infection in Japan. We tested 113 serum samples from wild boar captured in Shimonoseki City, Yamaguchi Prefecture, Japan from 2010 to 2012. Serum samples were tested by enzyme-linked immunosorbent assay (ELISA) using virus-like particles as antigen and nested reverse-transcription PCR (RT-PCR). Anti-HEV IgG antibodies were detected in 47 of the 113 wild boar serum samples (42%), and HEV RNA was detected in five samples (4%). Sequence analysis showed that the five HEV isolates belonged to genotype 4, forming a cluster with a previous isolate from a human hepatitis E case in this region in 2011. These results indicate that wild boar in this region are infected with potentially pathogenic HEV at a high prevalence.

show abstract

“…Rabbit fecal samples collected from a rabbit farm in Virginia tested positive for HEV RNA by a nested reverse transcription-PCR using degenerate primers (2). Overlapping fragments covering the complete genome of U.S. rabbit HEV were subsequently amplified using the primer walking strategy.…”

mentioning

confidence: 99%

Complete Genome Sequence of Hepatitis E Virus from Rabbits in the United States

Cossaboom

Córdoba

Cao

et al. 2012

J Virol

Self Cite

View full text Add to dashboard Cite

Hepatitis E virus (HEV) is a single-strand positive-sense RNA virus in the family Hepeviridae. The disease caused by HEV, hepatitis E, is an important public health problem in developing countries of Asia and Africa and is also endemic in many industrialized countries, including the United States. HEV has been identified from several other animal species in addition to humans, including the pig, chicken, mongoose, deer, rabbit, ferret, bat, and fish. Here we report the complete genome sequence of the first strain of HEV from rabbits in the United States. Sequence and phylogenetic analyses revealed that the U.S. rabbit HEV is a distant member of the zoonotic genotype 3 HEV, thus raising a concern for potential zoonotic human infection. A unique 90-nucleotide insertion within the X domain of the ORF1 was identified in the rabbit HEV, and this insertion may play a role in the species tropism of HEV.

show abstract

Hepatitis E Virus in Rabbits, Virginia, USA

Cited by 143 publications

References 16 publications

High Prevalence of Hepatitis E in Humans and Pigs and Evidence of Genotype-3 Virus in Swine, Madagascar

High Prevalence of Hepatitis E in Humans and Pigs and Evidence of Genotype-3 Virus in Swine, Madagascar

High Prevalence of Hepatitis E Virus in Wild Boar (Sus scrofa) in Yamaguchi Prefecture, Japan

Complete Genome Sequence of Hepatitis E Virus from Rabbits in the United States

Contact Info

Product

Resources

About