Hepatocyte Growth Factor in Mouse Soleus Muscle Increases with Reloading after Unloading

Tanaka, Shoji; Tachino, Katsuhiko; Kawahara, Ei; Tanaka, Junji; Funakoshi, Hiroshi; Nakamura, Toshikazu

doi:10.1589/jpts.18.33

Cited by 13 publications

(12 citation statements)

References 52 publications

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“…In this study, HGF levels were 3.4-6.2 ng in the gastrocnemius muscle at 4-24 weeks of age. These data are comparable with data from recent study 17) . HGF protein levels in rat skeletal muscles were detected until 24 weeks old.…”

Section: Discussionsupporting

confidence: 92%

“…HGF protein levels in rat skeletal muscles were detected until 24 weeks old. HGF promotes the proliferation of adult myoblasts 18) and is present in adult tibialis anterior 13) , soleus and plantaris muscles 17) . However, according to northern blot analysis, HGF mRNA signals could be detected until 10 days after birth, but could not be detected in adult skeletal muscle 19) .…”

Section: Discussionmentioning

confidence: 99%

“…Activated satellite cells as identified by BrdUpositive nuclei were noted inside the sarcolemma, as indicated by dystrophin signals. Recent studies have reported that mechanical stretching 13) and reloading after hindlimb suspension 17) stimulate satellite cell activation and production of HGF, and treadmill running stimulates satellite cell activation 3,20) . No recent studies have undertaken an examination of satellite cells in association with differing exercise training.…”

Section: Discussionmentioning

confidence: 99%

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Differing Responses of Satellite Cell Activity to Exercise Training in Rat Skeletal Muscle

et al. 2009

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Abstract.[Purpose] The present study examined satellite cell and hepatocyte growth factor (HGF) responses to exercise intensity in the rat soleus muscle.[Methods] HGF levels assessed during postnatal growth of the gastrocnemius muscle. Depression of HGF levels occurred up to postnatal week 4, so 4-weekold rats were used the exercise training experiment. Rats walked or ran at speeds of 16 or 24 m/min, at -16% grade, 30 min/trial. Soleus muscles were removed after 72 h. Animals were injected with the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU) 1 h before sampling. The right soleus muscle was used for immunofluorescence, and the left soleus muscle was used to measure HGF protein levels.[Result] HGF levels were unchanged, although numbers of BrdU-positive nuclei increased 2.4-fold in rats with exercise training.[Conclusion] The relationship between activation of satellite cells and HGF production after exercise training remains unclear. However, this study indicates the exercise intensity necessary to activate satellite cells. In the future, this result may facilitate the creation of exercise training intensity as an index of satellite cell activity for muscle strength training.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Differing Responses of Satellite Cell Activity to Exercise Training in Rat Skeletal Muscle

et al. 2009

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“…; Tanaka et al . ) and that their major sources may be activated, including proliferating satellite cells (Alterio et al . ; Lafyatis et al .…”

Section: Discussionmentioning

confidence: 99%

Time‐coordinated prevalence of extracellular HGF, FGF2 and TGF‐β3 in crush‐injured skeletal muscle

Suzuki

Borjigin

et al. 2012

Animal Science Journal

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Successful regeneration and remodeling of neuromuscular junctions are critical for restoring functional capacities and properties of skeletal muscle after damage, and axon-guidance molecules may be involved in the signaling that regulates such restoration. Recently, we found that early-differentiated satellite cells up-regulate a secreted neural chemorepellent Sema3A upon in vivo muscle-crush injury. The study also revealed that Sema3A expression is up-regulated in primary satellite-cell cultures in response to hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) and is prevented by transforming growth factor (TGF)-β2, 3. In order to verify the physiological significance of this regulation in vitro, the present study was designed to estimate the time-course of extracellular HGF, FGF2 and TGF-β3 concentrations after crush-injury of Gastrocnemius muscle in the rat lower hind-limb, using a combination of a non-homogenization/non-spin extraction of extracellular wound fluids and enhanced chemiluminescence-Western blotting analyses. Results clearly demonstrated that active HGF and FGF2 are prevalent in 2-8 days post-crush, whereas active TGF-β3 increases after 12 days, providing a better understanding of the time-coordinated levels of HGF, FGF2 and TGF-β3 that drive regulation of Sema3A expression during regenerative intramuscular moto-neuritogenesis.

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“…Although the precise mechanism of satellite cell activation induced by hepatocyte growth factor (HGF) is unclear, it is one of the key events in this recovery process 1 , 2 , 3 , 4 , 5 ) . In fact, previous studies using experimental animal models have demonstrated that satellite cell activation and HGF production are induced by activities that cause continuous muscle contraction, such as high-intensity running for 30 min, synergist muscle ablation, contralateral continuous overloading by ablation of a sciatic nerve, and reloading 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ) . However, in clinical physical therapy, it is difficult to perform high-intensity exercise for 30 min or longer.…”

Section: Introductionmentioning

confidence: 99%

Myogenic cell response to muscle contraction with short electrical stimulation

2015

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[Purpose] The present study aimed to determine the effects of short muscle strength exercise on hepatocyte growth factor expression and satellite cell activation. [Subjects] The study included 72 2–12-week-old male Sprague-Dawley rats. [Methods] The rat plantaris muscle was contracted with a 5-min electrical stimulation of the sciatic nerve, and then, the mRNA expressions of hepatocyte growth factor and myogenic regulatory factors in the plantaris muscle were determined, and the phosphorylation of the hepatocyte growth factor receptor (c-Met) was examined. [Results] The mRNA expressions of hepatocyte growth factor and myogenic regulatory factors increased after a short muscle contraction compared to that un-contraction. Immunofluorescence analysis showed the expression of hepatocyte growth factor protein and the possibility that downstream biological changes occurred in the hepatocyte growth factor-bound c-Met. [Conclusion] Our results demonstrated that activation of satellite cells induced hepatocyte growth factor expression during muscle contraction with a short 5-min electrical stimulation, which simulates short muscle strength exercise in physical therapy. The present study provides evidence for the use of short muscle strength exercise in physical therapy.

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Hepatocyte Growth Factor in Mouse Soleus Muscle Increases with Reloading after Unloading

Cited by 13 publications

References 52 publications

Differing Responses of Satellite Cell Activity to Exercise Training in Rat Skeletal Muscle

Differing Responses of Satellite Cell Activity to Exercise Training in Rat Skeletal Muscle

Time‐coordinated prevalence of extracellular HGF, FGF2 and TGF‐β3 in crush‐injured skeletal muscle

Myogenic cell response to muscle contraction with short electrical stimulation

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