Heptafluorobutyl Chloroformate-Based Sample Preparation Protocol for Nonchiral and Chiral Amino Acid Analysis by Gas Chromatography-Mass Spectrometry

Šimek, Petr; Hušek, Petr; Zahradníčková, Helena

doi:10.1007/978-1-4939-9639-1_18

Cited by 2 publications

(1 citation statement)

References 6 publications

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“…Generally, the determination of Cys is carried out by spectrophotometry, [7] fluorimetry, [8] chemiluminescence, [9] gas chromatography-mass spectrometry (GC-MS) [10] and high-performance liquid chromatography (HPLC). [11] These methods have some limitations such as poor selectivity, required longer time for analysis, sample pre-treatments, expensive equipment's, etc.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Stable CuO/Boron Nitride Nanocomposite Modified Electrode for Selective Electrochemical Detection of L–Cysteine

et al. 2020

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L-cysteine (Cys) is one of the main amino acid and its deficiency could cause several diseases and biological disorders in our body. Thus, it is important to accurately detect Cys level in biological samples. Herein, we reported a facile strategy to fabricate novel electrode material consisting of conductive hybrid film made of copper oxide-boron nitride nanosheets (CuO/BN). Powdered X-ray diffraction (PXRD) and Fourier transform infrared (FT-IR) spectroscopy results confirmed the successful formation of CuO/BN hybrid nanocomposite. Next, electrochemical and electro-catalytic properties of CuO/BN hybrid film is studied by cyclic voltammetry and amperometry. Interestingly, CuO/BN coated glassy carbon electrode (GCE) showed excellent electro-catalytic activity towards Cys oxidation in phosphate buffer solution (0.1 M PBS). Newly developed CuO/BN film modified electrode is exhibited high oxidation peak current at lower potential of + 0.45 V compared to bare GCE. CuO/BN/GCE sensor is also utilized for the detection of Cys from 1 to 10 μM and limit of detection (LOD) was found to be 0.58 μM. The selectivity of the sensor is determined by detecting 5 μM Cys in the presence of different interferent molecules. Furthermore, repeatability, reproducibility and stability of the CuO/BN/GCE sensor are also investigated. Finally, CuO/BN/GCE sensor is successfully applied to detect spiked Cys in human blood serum samples with good recovery.

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Section: Introductionmentioning

confidence: 99%

Preparation of Stable CuO/Boron Nitride Nanocomposite Modified Electrode for Selective Electrochemical Detection of L–Cysteine

et al. 2020

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Recent Advances in Chiral Analysis for Biosamples in Clinical Research and Forensic Toxicology

2021

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This article covers current methods and applications in chiral analysis from 2010 to 2020 for biosamples in clinical research and forensic toxicology. Sample preparation for aqueous and solid biological samples prior to instrumental analysis were discussed in the article. GC, HPLC, capillary electrophoresis and sub/supercritical fluid chromatography provide the efficient tools for chiral drug analysis coupled to fluorescence, UV and MS detectors. The application of chiral analysis is discussed in the article, which involves differentiation between clinical use and drug abuse, pharmacokinetic studies, pharmacology/toxicology evaluations and chiral inversion. Typical chiral analytes, including amphetamines and their analogs, anesthetics, psychotropic drugs, β-blockers and some other chiral compounds, are also reviewed.

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Heptafluorobutyl Chloroformate-Based Sample Preparation Protocol for Nonchiral and Chiral Amino Acid Analysis by Gas Chromatography-Mass Spectrometry

Cited by 2 publications

References 6 publications

Preparation of Stable CuO/Boron Nitride Nanocomposite Modified Electrode for Selective Electrochemical Detection of L–Cysteine

Preparation of Stable CuO/Boron Nitride Nanocomposite Modified Electrode for Selective Electrochemical Detection of L–Cysteine

Recent Advances in Chiral Analysis for Biosamples in Clinical Research and Forensic Toxicology

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