Heptaplex-direct PCR assay for simultaneous detection of foodborne pathogens

Hasap, Laila; Thanakiatkrai, Phuvadol; Linacre, Adrian; Kitpipit, Thitika

doi:10.1016/j.fsigss.2017.09.063

Cited by 1 publication

(1 citation statement)

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“…Some previous mPCR and multiplex RT-PCR assays for simultaneous detection of E. coli O157, L. monocytogenes and Salmonella spp. have been developed [ 7 , 8 , 9 , 10 , 11 ], while only one of the mRT-PCR assays for simultaneous detection of STEC, L. monocytogenes and Salmonella spp. was recently reported [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

In-House Validation of Multiplex PCR for Simultaneous Detection of Shiga Toxin-Producing Escherichia coli, Listeria monocytogenes and Salmonella spp. in Raw Meats

Jaroenporn

Supawasit

Bundidamorn

et al. 2022

Foods

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The aim of the study was to perform in-house validation of the developed multiplex PCR (mPCR)-based alternative method to detect Shiga toxin-producing Escherichia coli (STEC), Listeria monocytogenes (L. monocytogenes) and Salmonella spp. in raw meats following the ISO 16140-2: 2016. A comparative study of the developed mPCR against the Bacteriological Analytical Manual (BAM) method was evaluated for inclusivity and exclusivity, sensitivity and the relative level of detection (RLOD). Inclusivity levels for each target bacterium were all 100%, while exclusivity for non-target bacteria was 100%. The sensitivity of the developed mPCR was calculated based on the analysis of 72 samples of raw meat. The sensitivity of the developed mPCR was 100%. The RLOD values of the developed mPCR for STEC, L. monocytogenes and Salmonella spp. were 0.756, 1.170 and 1.000, respectively. The developed mPCR showed potential as a tool for the fast, specific and sensitive detection of the three bacteria in the raw meat industry

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