The goal of the current study was to assess the efficacy of powder Harbominvit in non-infectious repeat breeder buffaloes with a selective test such as white side test, cervical pH, PMNs cell count, Fern patten and estimation of serum phosphorous. 24 buffaloes that tested negative for white side test was chosen for the current study out of a total of 51 buffaloes that underwent the test from Nanded and Parbhani districts of Marathwada region in Maharashtra under infertility camps organized by NDDB, Mother dairy, and Collage of animal and veterinary science Parbhani. MAFSU. In the Treatment group at day 0 before treatment repeat breeder buffaloes showed a Mean ± SE cervical pH value was 7.38±0.07 while the Control group showed a Mean ± SE cervical pH value was 7.59±0.08. The white side test was 100% negative for both groups. The mean ± SE value for PMN cell count for the Treatment group is 2.67±0.31 while the Control group was 2.08±0.22. The mean ± SE value for serum phosphorus value for the Treatment group before treatment is 3.81a±0.11 and after treatment is 4.937a±0.18 while the Control group shows before 3.68a±0.09 and after treatment 4.17a±0.20 and mean ± SE value for Fern pattern for Treatment group and Control group was 0.750±0.13 and 0.66±0.14 respectively. The conception rates in buffaloes of Group 1 with the treatment of powder Harbominvit and Group 2 with no treatment serve as control were 75% (09/12), and 41.66 % (05/12) respectively (Table 2). The overall conception rate in the present study was 58.33% (14/24) in non-infectious repeat-breeding buffaloes. As an herbal combination with chelated minerals and some vitamins has best conception rate in non-infectious repeat breeder buffaloes having the advantage of reducing the cost of treatment in repeat breeding as compared to other treatment protocols.