Herbal hydrogel‐based encapsulated Enterococcus faecium ABRIINW.N7 improves the resistance of red hybrid tilapia against Streptococcus iniae

Abstract

Aims: The streptococcal disease has been associated with serious mortality and significant global economic loss in the tilapia farming industry. The overall goal of this work was to test herbal hydrogels based on encapsulated Enterococcus faecium ABRIINW.N7 for potential probiotic anti-microbial activity against Streptococcus iniae in red hybrid tilapia. Methods and Results: Abnormal behaviour, clinical signs, postinjection survival and histopathology (kidney, liver, eye and brain) were measured. Cumulative mortality of CON + , free cells, ALG and treatments (F1-F7) was 30, 24, 22, 19, 17, 17, 16, 14, 14 and 12 out of 30 fish and the survival rates for E. faecium ABRIINW.N7 microencapsulated in an alginate-BS blend with 0Á5, 1, 1Á5, 2, 2Á5 and 3% fenugreek were 43, 43, 47, 53, 53 and 60%, respectively. After the incorporation of fenugreek with the alginate-BS blend, there was an 8-21% increase in probiotic cell viability. Furthermore, the survival rate for the alginate-BS blend with 2Á5 and 3% fenugreek (F6 and F7) was significantly (P ≤ 0Á05) higher than other blends. The highest encapsulation efficiency, viability in gastrointestinal conditions and during storage time and excellent antipathogenicity against S. iniae were observed in alginate-BS +3% fenugreek formulation (F7). Conclusions: It is recommended that probiotic strains like E. faecium ABRIINW.N7 in combination with local herbal gums, such as BS and fenugreek plus alginate, can be used as a suitable scaffold and an ideal matrix for the encapsulation of probiotics. Significance and Impact of the Study: This study proposes models connecting process parameters, matrix structure and functionality.

Effective encapsulation of reuterin-producing Limosilactobacillus reuteri in alginate beads prepared with different mucilages/gums

“…Similar to our results, Kahieshesfandiari et al. [48] and Nami et al. [23] reported the encapsulation by extrusion of Enterococcus faecium ABRIINW.N7 and Lactococcus lactis ABRIINW-N19 in alginate-basil-fenugreek and alginate-persian gum, respectively.…”

Probiotic potential characterization and clustering using unsupervised algorithms of lactic acid bacteria from saltwater fish samples

“…The viability of selected strains in simulated gastrointestinal conditions was measured based on method described by Nami et al (2019) 17 . For this purpose, cells cultured overnight were collected by centrifugation at 1500 × g at 4 °C for 15 min.…”

“…Among different lactic acid bacteria (LAB), the probiotic effects on fish were confirmed for Lactobacillus pentosus 15 , Lactobacillus paraplantarum 16 , Enterococcus faecium 17 , Enterococcus faecalis 18 , Pediococcus pentosaceus 19 , and Lactococcus lactis 20 . However, identification and characterization of new strains with desired features are inevitable 21 .…”

Soy lecithin increases the stability and lipolysis of encapsulated algal oil and probiotics complex coacervates

“…In addition, probiotics also possess the potential to treat clinical symptoms, which has allowed them to also find a place in pharmaceutical and drug industries. 10 Microencapsulated probiotics can overcome various pharmaceutical and biological barriers, maximizing their therapeutic effectiveness and clinical applicability. 11 For probiotic microencapsulation, complex coacervation has been shown to be an effective technology and has various advantages conducive to probiotic encapsulation, such as high encapsulation efficiency, low cost and mild preparation conditions.…”