Herpes simplex virus 1 infection alters the mRNA translation processing in L-02 cells

Hong, Min; Che, Yanchun; Tang, Gui-zhen; Wei, Cun; Zhang, Xuemei; Liu, Long-ding; Li, Qihan

doi:10.1007/s12250-008-2903-5

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…Our results also showed that infection of CSFV increased cellular RPLP1 expression ( Figure 5 ), which could be benefit to enhance CSFV propagation due to the positive role of RPLP1 in CSFV growth. Similar to our findings, RPLP1 was also observed to be significantly up-regulated after HSV-1 infection in L-02 cells [ 59 ]. However, whether the up-regulation of RPLP1 expression in CSFV-infected cells depends on NS4B needs more study.…”

Section: Discussionsupporting

confidence: 91%

RPLP1, an NS4B-interacting protein, enhances production of CSFV through promoting translation of viral genome

et al. 2022

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Classical swine fever virus (CSFV), the etiological agent of classical swine fever (CSF), causes serious financial losses to the pig industry. Using yeast two-hybrid screening, we have previously identified ribosomal protein RPLP1 as a potential binding partner of CSFV NS4B. In this study, the interaction between host RPLP1 and CSFV NS4B was further characterized by co-immunoprecipitation (co-IP), glutathione S-transferase (GST) pulldown, and confocal microscopy. In addition, lentivirus-mediated shRNA knockdown of RPLP1 drastically attenuated CSFV growth, while stable overexpression of RPLP1 markedly enhanced CSFV production. Moreover, cellular RPLP1 expression was found to be significantly up-regulated along with CSFV infection. Dual-luciferase reporter assay showed that depletion of RPLP1 had no effects on the activity of CSFV internal ribosome entry site (IRES). In the first life cycle of CSFV, further studies revealed that RPLP1 depletion did not influence the intracellular viral RNA abundance but diminished the intracellular and extracellular progeny virus titers as well as the viral E2 protein expression, which indicates that RPLP1 is crucial for CSFV genome translation. In summary, this study demonstrated that RPLP1 interacts with CSFV NS4B and enhances virus production via promoting translation of viral genome.

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Section: Discussionsupporting

confidence: 91%

RPLP1, an NS4B-interacting protein, enhances production of CSFV through promoting translation of viral genome

et al. 2022

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“…Several cellular molecules have negative functions with respect to the regulation of viral gene transcription. This differs from the conventional interferon response, which restricts viral replication by affecting the cellular transcriptional regulatory system [21,30,31]. In this study, we aimed to determine which cellular and viral molecules are involved in the transcriptional regulation of HSV-1.…”

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confidence: 99%

A cellular response protein induced during HSV-1 infection inhibits viral replication by interacting with ATF5

Zhang

Che

et al. 2013

Sci. China Life Sci.

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Studies of herpes simplex virus type 1 (HSV-1) infection have shown that many known and unknown cellular molecules involved in viral proliferation are up-regulated following HSV-1 infection. In this study, using two-dimensional polyacrylamide gel electrophoresis, we found that the expression of the HSV-1 infection response repressive protein (HIRRP, GI 16552881) was up-regulated in human L02 cells infected with HSV-1. HIRRP, an unknown protein, was initially localized in the cytoplasm and then translocated into the nucleus of HSV-1-infected cells. Further analysis showed that HIRRP represses HSV-1 proliferation by inhibiting transcription of the viral genome by interacting with the cellular transcription factor, ATF5, via its N-terminal domain. ATF5 represses the transcription of many host genes but can also act as an activator of genes containing a specific motif. We found that ATF5 promotes the proliferation of HSV-1 via a potential mechanism by which ATF5 enhances the transcription of viral genes during the course of an HSV-1 infection; HIRRP then induces feedback repression of this transcription by interacting with ATF5.

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Herpes simplex virus 1 infection alters the mRNA translation processing in L-02 cells

Cited by 2 publications

References 17 publications

RPLP1, an NS4B-interacting protein, enhances production of CSFV through promoting translation of viral genome

RPLP1, an NS4B-interacting protein, enhances production of CSFV through promoting translation of viral genome

A cellular response protein induced during HSV-1 infection inhibits viral replication by interacting with ATF5

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