Herpes simplex virus ophthalmic disease induced using two different methods of mice inoculation

Pereira, Max Domingues; Ma, Guimarães; Lv, Neto; Segenreich, Daniel; Varella, RB; Vl, Antunes Chagas; Fp, Câmara

doi:10.1590/s1413-86702001000400004

Cited by 6 publications

(11 citation statements)

References 10 publications

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“…Viral inoculum was prepared as described previously in our studies 10,11 . Three-week-old male BALB/ c mice were maintained as a breeding colony in the Virology Department of the Federal University of Rio de Janeiro (RJ, Brazil), and were used in this study.…”

Section: Virus and Mice Infectionmentioning

confidence: 99%

“…It is known that HSV-1 produces a diversity of clinical syndromes in the eye. The most common consists of a keratitis frequently followed by conjunctivitis and blepharitis 3,6,10,11,17 . In the event that is limited to the corneal epithelium, the pathogenesis appears to be due to viral cell destruction resulting in corneal ulceration 3,6,10,11,17 .…”

Section: Introductionmentioning

confidence: 99%

“…The most common consists of a keratitis frequently followed by conjunctivitis and blepharitis 3,6,10,11,17 . In the event that is limited to the corneal epithelium, the pathogenesis appears to be due to viral cell destruction resulting in corneal ulceration 3,6,10,11,17 . However, sometimes, episodes of herpetic ophthalmic disease result in the involvement of the deeper corneal tissue leading to corneal scarring with consequent reduction in visual acuity.…”

Section: Introductionmentioning

confidence: 99%

“…However, sometimes, episodes of herpetic ophthalmic disease result in the involvement of the deeper corneal tissue leading to corneal scarring with consequent reduction in visual acuity. Additionally, delay in the treatment of HSV-1 ophthalmic disease can lead to a more significant corneal scar, and topical steroid treatment in unsuspected active herpes ophthalmic can lead to corneal melting 3,4,6,10,11,15 .…”

Section: Introductionmentioning

confidence: 99%

“…Essentially the present information regarding the ophthalmic herpes comes from studies done in laboratory animals 4,5,10,11,15 . Therefore, we had previously optimized a murine model of HSV-1 ophthalmic disease, in which BALB/c mouse corneas were inoculated without trauma and with HSV-1 strain obtained from a patient with severe herpetic disease, to mimic the infection in humans 4,5,10,11,15 .…”

Section: Introductionmentioning

confidence: 99%

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An immunofluorescence test for diagnosis of ophthalmic herpes in a mouse corneal model

Pereira

Câmara

Guimarães

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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SUMMARYHerpes simplex virus type 1 (HSV-1) ophthalmic disease is the most common cause of corneal blindness in humans worldwide. Current culture techniques for HSV take several days and commercially available HSV laboratory based diagnostic techniques vary in sensitivity. Our study was conducted to evaluate the use of a quicker and simpler method to herpes ophthalmic diagnosis. Corneal smears were made by firm imprints of infected mouse eyes to glass slides, after smears were fixated with cold acetone, and an indirect immunofluorescence (IIF) method was performed using monoclonal antibodies in a murine model of ophthalmic herpes. Eye swabs from infected mice were inoculated in Vero cells for virus isolation. Cytology and histology of the eye were also performed, using hematoxylin-eosin routine. Mouse eyes were examined by slit-lamp biomicroscopy for evidence of herpetic disease at various times postinoculation. We made a comparative evaluation of sensitivity, specificity and speed of methods for laboratory detection of HSV. Our results indicate that this IIF method is quick, sensitive, specific and can be useful in the diagnosis of ophthalmic herpes as demonstrated in an animal model.

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Section: Virus and Mice Infectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

An immunofluorescence test for diagnosis of ophthalmic herpes in a mouse corneal model

Pereira

Câmara

Guimarães

et al. 2007

Rev. Inst. Med. trop. S. Paulo

Self Cite

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Dry eye symptoms in interferon regulatory factor 3-deficient mice due to herpes simplex virus infection in harderian gland and lacrimal gland

Zhu

Zhang

Zheng

et al. 2022

Experimental Eye Research

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Prior inhibition of AKT phosphorylation by BX795 can define a safer strategy to prevent herpes simplex virus-1 infection of the eye

et al. 2020

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Purpose-To evaluate the prophylactic antiviral efficacy, corneal tolerance and toxicity of topically dosed BX795, a non-nucleoside small-molecule inhibitor of herpes simplex virus type-1 (HSV-1). Methods-Prophylactic treatment with BX795 was performed both in-vitro on human corneal epithelial cells and in-vivo on mice prior to HSV-1 challenge. Viral burden was evaluated using a standard plaque assay. In a separate experiment, mice were treated topically 3-times daily for 4weeks with BX795 to evaluate corneal tolerance and toxicity. Phenol-red thread measurements, fluorescein staining and optical coherence tomography (OCT) were used to evaluate tear production, dryness and corneal structural changes. Corneal sensitivity and intraocular pressure were measured using esthesiometery and tonometery respectively. Results-Both in-vitro and in-vivo results showed a robust suppression of HSV-1 infection when treated prophylactically with BX795. The fluorescein stain and phenol-red results for the BX795treated eyes did not show signs of corneal surface dryness when compared to trifluridine (TFT), an FDA-approved topical antiviral. The OCT measurements showed no signs of structural changes to the cornea suggesting that BX795 treatment was well tolerated without any apparent signs of toxicity or inflammation. The corneal sensitivity of BX795-treated eyes was not significantly different from TFT-treated eyes. No significant increase in the intraocular pressure of BX795treated mice was observed. Conclusions-Prophylactic treatment with BX795 protects corneal cells from HSV-1 infection. The antiviral is well-tolerated on murine corneas without any detectable toxicity.

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Herpes simplex virus ophthalmic disease induced using two different methods of mice inoculation

Cited by 6 publications

References 10 publications

An immunofluorescence test for diagnosis of ophthalmic herpes in a mouse corneal model

An immunofluorescence test for diagnosis of ophthalmic herpes in a mouse corneal model

Dry eye symptoms in interferon regulatory factor 3-deficient mice due to herpes simplex virus infection in harderian gland and lacrimal gland

Prior inhibition of AKT phosphorylation by BX795 can define a safer strategy to prevent herpes simplex virus-1 infection of the eye

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