Herpesvirus saimiri small RNA and interleukin-4 mRNA AUUUA repeats compete for sequence-specific factors including a novel 70K protein

Geck, Peter; Medveczky, Maria M.; Chou, Chin-Sheng; Brown, Ariana; Cus, Jolan; Medveczky, Peter G.

doi:10.1099/0022-1317-75-9-2293

Cited by 8 publications

(3 citation statements)

References 51 publications

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“…Much work has focused on understanding the roles of RNAs in RNase P (2) and telomerase (3) action, of small nuclear RNAs (snRNAs) in mRNA splicing (4,5), and of small nucleolar RNAs (snoRNAs) in rRNA maturation (6)(7)(8)). Yet ncRNAs have also been implicated in the processes of transcription (9), translation (10), transport (11), RNA editing (12), mRNA stability (13), differentiation (14) and protein degradation (15). For example, the Xist ncRNA of mammals is critical for X chromosome inactivation in females (16), while the Drosophila roX1 ncRNA localizes to the male X chromosome and is potentially involved in dosage compensation (17).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the yeast genome: identification of new non-coding and small ORF-containing RNAs

Olivas

Muhlrad

Parker

1997

Nucleic Acids Research

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The genome sequences from increasing numbers of organisms allow for rapid and organized examination of gene expression. Yet current computational-based paradigms for gene recognition are limited and likely to miss genes expressing non-coding RNAs or mRNAs with small open reading frames (ORFs). We have utilized two strategies to determine if there are additional transcripts in the yeast Saccharomyces cerevisiae that were not identified in previous analyses of the genome. In one approach, we identified strong consensus polymerase III promoters based on sequence, and determined experimentally if these promoters drive the expression of an RNA polymerase III transcript. This approach led to the identification of a new, non-essential 170 nt non-coding RNA. An alternative strategy analyzed RNA expression from large sequence gaps>2 kb between predicted ORFs. Fifteen unique RNA transcripts ranging in size from 161 to 1200 nt were identified from a total of 59 sequence gaps. Several of these RNAs contain unusually small potential ORFs, while one is clearly non-coding and appears to be a small nucleolar RNA. These results suggest that there are likely to be additional previously unidentified non-coding RNAs in yeast, and that new paradigms for gene recognition will be required to identify all expressed genes from an organism.

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Section: Introductionmentioning

confidence: 99%

Analysis of the yeast genome: identification of new non-coding and small ORF-containing RNAs

Olivas

Muhlrad

Parker

1997

Nucleic Acids Research

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“…The AU-rich sequences of certain mRNAs have been implicated in tumorigenesis. It has been proposed that AU-rich viral RNA sequences directly affect cell transformation (12,28). It was proposed that U RNAs produced by herpesvirus saimiri compete directly with AU-rich elements of cellular proto-oncogene mRNAs for factors that mediate degradation of the cellular proto-oncogene mRNAs.…”

Section: Discussionmentioning

confidence: 99%

Identification of nuclear and cytoplasmic proteins that interact specifically with an AU-rich, cis-acting inhibitory sequence in the 3' untranslated region of human papillomavirus type 1 late mRNAs

Zhao

Tan

Sokolowski

et al. 1996

J Virol

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Expression of human papillomavirus late genes encoding L1 and L2 capsid proteins is restricted to terminally differentiated epithelial cells. We have previously identified and characterized an AU-rich, cis-acting negative regulatory element in the 3 untranslated region of human papillomavirus type 1 late mRNAs. This element acts posttranscriptionally to reduce mRNA levels and the translation efficiency of mRNAs. The experiments reported here are a continuation of our previous work. We have used RNA gel shifts and UV cross-linking assays to identify cellular proteins that interact with the inhibitory RNA sequence of human papillomavirus type 1. RNA gel shift assays established that cellular proteins interact with the AU-rich sequence. The binding of nuclear proteins was inhibited by competition with poly(U), whereas the binding of cytoplasmic proteins was inhibited by competition with poly(U) and also by competition with poly(A) and poly(G). Two nuclear proteins and two cytoplasmic proteins that bind specifically to the AU-rich RNA sequence were identified by UV cross-linking. These proteins did not bind to the 3 untranslated region of human papillomavirus type 1 early mRNAs, which does not show inhibitory activity. The cellular proteins identified in our experiments may therefore be involved in the inhibition of human papillomavirus type 1 late gene expression in nondifferentiated epithelial cells.

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“…The rhadinovirus prototype, herpesvirus saimiri strain A11 (2), contains seven genes for virus-encoded small nuclear RNAs that have been termed herpesvirus saimiri URNAs (or HSURs 1 to 7) (1,16,20,25). The HSURs assemble into ribonucleoprotein particles (17) and can interact with cellular factors involved in mRNA destabilization (11,12,22). These 32-or 70-kDa proteins physiologically bind to AU-rich elements in the 3Ј untranslated regions of cellular mRNAs, which leads to the rapid degradation of the respective mRNAs.…”

mentioning

confidence: 99%

The URNA Genes of Herpesvirus Saimiri (Strain C488) Are Dispensable for Transformation of Human T Cells In Vitro

Ensser

Pfinder

Müller-Fleckenstein

et al. 1999

J Virol

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The herpesvirus saimiri strain C488 genome contains five genes for small nuclear RNAs, termed herpesvirus saimiri URNAs (or HSURs). Using a cosmid-based approach, all HSURs were precisely deleted from the genome. The mutant virus replicated at levels that were similar to those of wild-type viruses in OMK cells. Although the HSURs are expressed in wild-type virus-transformed human T-cell lines, the deletion does not affect viral transformation in cell culture.

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Herpesvirus saimiri small RNA and interleukin-4 mRNA AUUUA repeats compete for sequence-specific factors including a novel 70K protein

Cited by 8 publications

References 51 publications

Analysis of the yeast genome: identification of new non-coding and small ORF-containing RNAs

Analysis of the yeast genome: identification of new non-coding and small ORF-containing RNAs

Identification of nuclear and cytoplasmic proteins that interact specifically with an AU-rich, cis-acting inhibitory sequence in the 3' untranslated region of human papillomavirus type 1 late mRNAs

The URNA Genes of Herpesvirus Saimiri (Strain C488) Are Dispensable for Transformation of Human T Cells In Vitro

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