Heterogeneity among the 2μm plasmids in Saccharomyces cerevisiae: A new sequence for the REP1 gene

Neuville, Philippe; Bonneu, Marc; Aigle, Michel

doi:10.1016/0378-1119(90)90217-f

Cited by 2 publications

(1 citation statement)

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“…2μ sexual/non-Mendelian inheritance, stability, site-specific re-combination and copy number control in S. cerevisiae have been extensively studied; for reviews, see Futcher (1988), Jayaram et al (2004) and Chan et al (2013). 2μ is not present in all S. cerevisiae strains (Nakayashiki et al 2005;Kelly et al 2012) and exhibits sequence variation (Neuville, Bonneu and Aigle 1990;Wei, Pelcher and Rank 1991;Xiao, Pelcher and Rank 1991a,b;Rank, Xiao and Arndt 1994;Xiao and Rank 1996). Thus, 2μ presence/absence and sequence variation are both components of S. cerevisiae genetic variation and potentially so in other Saccharomyces species.…”

Section: Introductionmentioning

confidence: 99%

2μ plasmid inSaccharomycesspecies and inSaccharomyces cerevisiae

Strope

Kozmin

Skelly

et al. 2015

FEMS Yeast Research

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We determined that extrachromosomal 2μ plasmid was present in 67 of the Saccharomyces cerevisiae 100-genome strains; in addition to variation in the size and copy number of 2μ, we identified three distinct classes of 2μ. We identified 2μ presence/absence and class associations with populations, clinical origin and nuclear genotypes. We also screened genome sequences of S. paradoxus, S. kudriavzevii, S. uvarum, S. eubayanus, S. mikatae, S. arboricolus and S. bayanus strains for both integrated and extrachromosomal 2μ. Similar to S. cerevisiae, we found no integrated 2μ sequences in any S. paradoxus strains. However, we identified part of 2μ integrated into the genomes of some S. uvarum, S. kudriavzevii, S. mikatae and S. bayanus strains, which were distinct from each other and from all extrachromosomal 2μ. We identified extrachromosomal 2μ in one S. paradoxus, one S. eubayanus, two S. bayanus and 13 S. uvarum strains. The extrachromosomal 2μ in S. paradoxus, S. eubayanus and S. cerevisiae were distinct from each other. In contrast, the extrachromosomal 2μ in S. bayanus and S. uvarum strains were identical with each other and with one of the three classes of S. cerevisiae 2μ, consistent with interspecific transfer.

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