Heterogeneous microstructural changes of the cervix influence cervical funneling

Moghaddam, Amir Ostadi; Lin, Zixi; Sivaguru, Mayandi; Phillips, Heidi S.; McFarlin, Barbara L.; Toussaint, Kimani C.; Johnson, Amy J. Wagoner

doi:10.1016/j.actbio.2021.12.025

Cited by 16 publications

(2 citation statements)

References 59 publications

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“…It should be noted that the structural changes that occur during pregnancy are not universally seen in the entire cervix. Novel studies that use quantitative 3D second-harmonic generation microscopy have suggested that the reorientation of collagen fibers is predominantly seen in the cervical canal in the inner cervical zone [ 34 ]. It is important to mention that experimental studies also suggest that the remodeling process of preterm birth as a result of an inflammatory environment differs compared to the remodeling process that normally occurs at term pregnancy [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Expression of Stemness Markers in the Cervical Smear of Patients with Cervical Insufficiency

Pittokopitou

Mavrogianni

Pergialiotis

et al. 2023

Cells

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The presence of stem cells has been previously described in human precancerous and malignant cervical cultures. Previous studies have shown a direct interplay of the stem cell niche, which is present in practically every tissue with the extracellular matrix. In the present study, we sought to determine the expression of stemness markers in cytological specimens collected from the ectocervix among women with cervical insufficiency during the second trimester of pregnancy and women with normal cervical length. A prospective cohort of 59 women was enrolled of whom 41 were diagnosed with cervical insufficiency. The expression of OCT-4 and NANOG was higher in the cervical insufficiency group compared to the control group (−5.03 (−6.27, −3.72) vs. −5.81 (−7.67, −5.02) p = 0.040 for OCT4) and (−7.47 (−8.78, −6.27) vs. −8.5 (−10.75, −7.14), p = 0.035 for NANOG. Differences in the DAZL gene were not significantly different (5.94 (4.82, 7.14) vs. 6.98 (5.87, 7.43) p = 0.097). Pearson correlation analysis indicated the existence of a moderate correlation of OCT-4 and Nanog with cervical length. Considering this information, the enhanced activity of stemness biomarkers among pregnant women diagnosed with cervical insufficiency may be predisposed to cervical insufficiency, and its predictive accuracy remains to be noted in larger population sizes.

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Section: Discussionmentioning

confidence: 99%

Expression of Stemness Markers in the Cervical Smear of Patients with Cervical Insufficiency

Pittokopitou

Mavrogianni

Pergialiotis

et al. 2023

Cells

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show abstract

“…Then with a 40x 1.2 NA objective water immersion lens, volumetric images (141.7*141.7*20 μm) of the myometrium were obtained. The SHG parameters including out-of-plane fiber angle (ϕ), circular variance (CV), and spherical variance (SV) were computed for each sample using the orientation data provided by FT-SHG analysis (34,35). ϕ represents the average angle of the collagen fibers with respect to the x-y imaging plane, where 0° and 90° indicate in-plane and out-of-plane, respectively.…”

Section: Second Harmonic Generation (Shg) Imaging and Image Quantific...mentioning

confidence: 99%

Chronic exposure of mice to phthalates enhances TGF beta signaling and promotes uterine fibrosis

Shukla,

Arshee,

Laws

et al. 2023

Reproductive Toxicology

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Three-dimensional anisotropic hyperelastic constitutive model describing the mechanical response of human and mouse cervix

Shi

Lee

et al. 2022

Acta Biomaterialia

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Heterogeneous microstructural changes of the cervix influence cervical funneling

Cited by 16 publications

References 59 publications

Expression of Stemness Markers in the Cervical Smear of Patients with Cervical Insufficiency

Expression of Stemness Markers in the Cervical Smear of Patients with Cervical Insufficiency

Chronic exposure of mice to phthalates enhances TGF beta signaling and promotes uterine fibrosis

Three-dimensional anisotropic hyperelastic constitutive model describing the mechanical response of human and mouse cervix

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