Heterologous expression, molecular studies and biochemical characterization of a novel alkaline esterase gene fromBacillus thuringiensisfor detergent industry

Abstract

Present study was aimed to clone and express the esterase encoding gene from Bacillus thuringiensis in E. coli BL21.

Heterologous expression and characterization of a novel thermostable and alkali stable recombinant lipase enzyme from Bacillus thuringensis into E. coliBL21(DE3) for detergent formulation

“…To evaluate the gene expression and molecular mass determination, SDS‐PAGE was used (Figure 2). Many reports are present on the use of SDS‐PAGE to measure the molecular mass of recombinant proteins (Basit et al, 2021; Gao et al, 2022; Hamid et al, 2021; Shariati et al, 2021; Zafar et al, 2022b).…”

“…Maximum expression of cloned lipase gene was obtained at pH 7.0 which also resulted in the maximum total protein contents which might be due to the maximum growth of bacterial cells as shown in Figure 3d. Many reports are present on the effect of pH of medium on the expression of recombinant genes (Castellanos‐Mendoza et al, 2014; Zafar et al, 2022b).…”

“…The addition of an esterase was inspired by its capacity to improve lipid removal from surfaces or materials, particularly fatty acid residues. A recombinant esterase enzyme from Bacillus thuringensis was used in this study (Zafar et al, 2022b). These lipids frequently cause problems during cleaning procedures because they can build tenacious hydrophobic layers that are tough to dissolve with ordinary detergents.…”

Immobilization of Hyperthermostable Carboxylesterase EstD9 from Anoxybacillus geothermalis D9 onto Polymer Material and Its Physicochemical Properties

“…Carboxylesterases are prevalent in nature [ 4 ]. Of all sources, bacterial carboxylesterase is the main biocatalyst used in practical applications, such as in the production of detergent, textile, paper and the synthesis of optically purified compounds [ 5 , 6 , 7 , 8 ]. Despite this versatility, the applied free enzyme generally displays poor stability against high temperature, pH, and some chemical reactions, such that environmental circumstances can alter its conformation, leading to either reversible or irreversible deactivation.…”