Heterologous expression, refolding and characterization of a salt activated subtilase from Pleurotus ostreatus

Eisele, Nadine; Linke, Diana; Nimtz, Manfred; Berger, Ralf G.

doi:10.1016/j.procbio.2011.06.013

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…A subgroup of subtilisin-like proteases from ascomycete and basidiomycete fungi, belonging to the pyrolysin family has been defined (Faraco et al, 2005). For instance, most proteases of the genus Pleu rotus feature the characteristics of alkaline subtilases (Eisele et al, 2011). This makes mushroom forming fungi a large, untapped pool of novel proteases with potential for controlling mycotoxigenic fungi.…”

Section: Bioactive Proteins From Mushroom Forming Fungi With Activity Against Mycotoxigenic Speciesmentioning

confidence: 99%

Using mushroom-forming fungi in preventing and reducing mycotoxins in cereal products

Savoie¹,

Mata²,

Atanasova‐Pénichon³

et al. 2019

Sci Fungorum

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Background: Biological control for cereal diseases caused by mycotoxigenic fungi is part of the alternative methods to be developed to secure food and feed production, recognizing the negative effects these fungi have on crop yield and their potential production of mycotoxins. Objective: Update recent knowledge about the potential of mushroom-forming fungi as biological control agents of mycotoxigenic fungi species, acting as antagonists on crop debris, as source of active extracts with antifungal and/or antimycotoxin properties and as detoxifying agents in solid-state fermentation processes of contaminated cereals. Methods: A literature review of the published works was carried out, the main topics were analyzed, as well as the species with the greatest potential of biological control agents of mycotoxingenic fungi. Results and conclusions: Mushroom forming fungi could be used as antagonists on crop debris or grains, as sources of active extracts with antifungal and/or antimycotoxin properties, and as detoxifying agents. An integration of these three potentials gives rise to an environment friendly process for production of both safe grains and edible mushrooms.

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Section: Bioactive Proteins From Mushroom Forming Fungi With Activity Against Mycotoxigenic Speciesmentioning

confidence: 99%

Using mushroom-forming fungi in preventing and reducing mycotoxins in cereal products

Savoie¹,

Mata²,

Atanasova‐Pénichon³

et al. 2019

Sci Fungorum

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“…The use of liquid cultures facilitates the purification of bioactives such as enzymes and polysaccharides [ 3 ]. The submerged culture of P. ostreatus in wheat gluten resulted in the secretion of proteases that noticeably increased the overall solubility of the medium [ 13 ].…”

Section: Proteases From Basidiomycetesmentioning

confidence: 99%

“…The secretome of P. sapidus has shown protease production and ligninolytic enzymes, but some points remained unidentified, indicating that new enzymes with potential biotechnological applications should be studied and identified in the species [ 65 ]. The secretome of P. ostreatus has shown the presence of proteases with a potential role in the regulation of other extracellular enzymes [ 13 ]. Metalloproteases are enzymes that are finely associated with physiological processes and they have been explored in studies of bacteria and mammals; however, there have been very few studies of the metalloproteases of basidiomycetes [ 68 ].…”

Section: Proteases From Pleurotus Sppmentioning

confidence: 99%

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Proteases of Wood Rot Fungi with Emphasis on the GenusPleurotus

Inácio

Ferreira

Araújo

et al. 2015

BioMed Research International

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Proteases are present in all living organisms and they play an important role in physiological conditions. Cell growth and death, blood clotting, and immune defense are all examples of the importance of proteases in maintaining homeostasis. There is growing interest in proteases due to their use for industrial purposes. The search for proteases with specific characteristics is designed to reduce production costs and to find suitable properties for certain industrial sectors, as well as good producing organisms. Ninety percent of commercialized proteases are obtained from microbial sources and proteases from macromycetes have recently gained prominence in the search for new enzymes with specific characteristics. The production of proteases from saprophytic basidiomycetes has led to the identification of various classes of proteases. The genus Pleurotus has been extensively studied because of its ligninolytic enzymes. The characteristics of this genus are easy cultivation techniques, high yield, low nutrient requirements, and excellent adaptation. There are few studies in the literature about proteases of Pleurotus spp. This review gathers together information about proteases, especially those derived from basidiomycetes, and aims at stimulating further research about fungal proteases because of their physiological importance and their application in various industries such as biotechnology and medicine.

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“…En conclusión se logró expresar enzima putativa Glycoside Hydrolase Family 1 proteina de Pleurotus ostreatus PC15 v1.0 en una cepa nativa de S. cerevisiae y confirmar su actividad BG. Adicionalmente, de acuerdo a la revisión bibliográfica llevada a cabo no se encontraron reportes para la expresión de BGs de Pelurotus osteatus, encontrándose sólo reportes de la expresión de otras enzimas como "subtilisina-like" [114], lacasas [29,58] o peroxidasas [115] provenientes de este hongo. En este sentido, este estudió corresponde al primer reporte de la expresión de una BG putativa de P. ostreatus en S. cerevisiae.…”

Section: Transformación De La Cepa S Cerevisiae Cmdm-puj Y Evaluación...unclassified

Expresión de enzimas recombinantes en levaduras para la degradación de residuos de crisantemo

Rincón¹

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El principal impedimento tecnológico para aumentar el uso de biomasa lignocelulósica en la producción de combustibles y químicos, es la falta de tecnologías de bajo costo y ambientalmente amigables que permitan superar las cualidades recalcitrantes de este material. Por esta razón, en este trabajo se evaluó la degradación de residuos de crisantemo empleando los extractos enzimáticos obtenidos a partir de microorganismos expresando las enzimas lacasa (Lac), endoglucanasa (EG), celobiohidrolasa (CBH) y p-glucosidasa (BG). Se construyeron vectores de expresión que contenían individualmente los genes BG de Pleurotus ostreatus y para las enzimas EG y CBH de Trichoderma reesei. Se obtuvieron clones de Saccharomyces cerevisiae CMDM-PUJ y Wickerhamomyces anomalus 54A, los cuales se evaluaron a escala de 55 ml_ y posteriormente a escala de bioreactor. Con estos extractos se realizó la degradación de residuos de crisantemo, empleando los resultados de peso seco y concentración de azucares reductores como variables respuesta. La glucosa obtenida a partir de estos ensayos se utilizó para la producción de etanol. Se lograron expresar las enzimas CBH y BG en W. anomalus y S. cerevisiae, respectivamente. Los extractos enzimáticos obtenidos a partir de estos clones se utilizaron para enriquecer el extracto enzimàtico de Penicillium sp. El uso del extracto enzimàtico enriquecido en combinación con el extracto de Lac recombinante permitió la degradación parcial del residuo de crisantemo, logrando una reducción de peso seco cercana al 10% y una concentración de azucares reductores de hasta 2 g/L. Sin embargo, la concentración de glucosa generada tras el proceso de degradación no fue la suficiente como para generar una cantidad importante de etanol en la fermentación utilizando las cepas nativas y recombinante de S. cerevisiae y W. anomalus.

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Heterologous expression, refolding and characterization of a salt activated subtilase from Pleurotus ostreatus

Cited by 7 publications

References 32 publications

Using mushroom-forming fungi in preventing and reducing mycotoxins in cereal products

Using mushroom-forming fungi in preventing and reducing mycotoxins in cereal products

Proteases of Wood Rot Fungi with Emphasis on the GenusPleurotus

Expresión de enzimas recombinantes en levaduras para la degradación de residuos de crisantemo

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