Heterothallism and Homothallism: A Study of 27 Isolates of Didymium Iridis, a True Slime Mold

Collins, O’Neil Ray

doi:10.1002/j.1537-2197.1976.tb11795.x

Cited by 17 publications

(1 citation statement)

References 25 publications

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“…The strains used in this study were Hon1-2 (mating type A 1 ), CR2-26 (A 6 ), Pan 2-16 (A 7 ), Pan3-1 (A 9 ), Pan3-3 (A 10 ), CR5-5 (A 2 ), CR6 (A 7 ), Pan1-66.62-10 (A 3 ) (Collins 1976), CR8-1 (A 12 ), CR8-4 (A 7 ) (Clark and Stephenson 1990), Gua2 (A 13 ) (Clark and Landolt 1993), which are all interbreeding members of the Central American A1 mating series (Clark et al 1991). Strain names Pan, Hon, CR and Gua refer the presumed country of origin, Panama, Honduras, Costa Rica and Guatemala respectively (many of the isolates were obtained from moist cultures of banana peels from the above countries).…”

Section: Methodsmentioning

confidence: 99%

RNA editing is absent in a single mitochondrial gene ofDidymium iridis

Hendrickson¹,

Silliker

2010

Mycologia

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An open reading frame (ORF) was found in the mitochondrial genome of the Pan2-16 strain of Didymium iridis that showed high similarity to the NADH dehydrogenase subunit 3 (nad3) gene in other organisms. So far all other typical mitochondrial genes identified in this organism require RNA editing to generate ORFs capable of directing protein synthesis. The D. iridis sequence was compared to the putative nad3 gene in the related myxomycete Physarum polycephalum, which would require editing. Based on this comparison, editing sites could be predicted for the P. polycelphalum gene that would result in the synthesis of a highly conserved ND3 protein between the two organisms. To determine the editing status of the nad3 gene in other D. iridis strains, PCR was used to amplify this region from eight other independent isolates of the A1 Central American interbreeding series. In each case a 378 base pair ORF was detected by PCR amplification and sequencing. Three patterns of sequence variation were observed; however all base substitutions were in the third codon position and silent with respect to the amino acids encoded. The distribution of the sequence variants was mapped geographically. The requirement for RNA editing in all other typical mitochondrial genes of D. iridis and P. polycephalum and the presence of RNA editing in the nad3 gene of P. polycephalum suggest that the D. iridis nad3 gene might have been edited at one time. We propose that the D. iridis nad3 gene may have lost the requirement for RNA editing by reverse transcription of an edited transcript that subsequently was inserted into the genome.

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Section: Methodsmentioning

confidence: 99%

RNA editing is absent in a single mitochondrial gene ofDidymium iridis

Hendrickson¹,

Silliker

2010

Mycologia

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Cytophotometric Measurement of Nuclear Dna in Seven Heterothallic Isolates of Didymium Iridis, a Myxomycete

Collins

Therrien

1976

American J of Botany

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Absorption cytophotometry was used to measure nuclear Feulgen‐DNA content of myxamoebae and Plasmodia in seven heterothallic isolates of Didymium iridis. Measurements of myxamoebal nuclei from clones of four isolates (Hon 1, Pan 1, Pan 2, and CR 5) gave a mean DNA value of 0.34, whereas the nuclei of Plasmodia which develop from each of the four intraisolate crosses had a mean value of 0.63. These values correspond to the 2C haploid level in myxamoebae and the 4C diploid level in Plasmodia. DNA values in two additional isolates (Pan 3 and CR 2) are much higher than the mean for the other five. Accordingly, it is proposed that these may be polyploid. The question of polyploidy in D. iridis and in other myxomycetes is evaluated. The seventh isolate, Ky 1, is taxonomically very close to D. nigripes and was not included in calculations of mean values for D. iridis.

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Studies on the Mating Systems of Eleven Species of Myxomycetes

Clark

Collins

1976

American J of Botany

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Genetic analysis of 20 isolates representing 11 species of Myxomycetes was carried out to determine whether they were heterothallic or homothallic. Each of the single isolates of Badhamia utricularis, Didymium minus, D. nigripes, and Physarum cinereum was found to be heterothallic, the first such reports for all of the species except D. nigripes. All isolates of Badhamia foliicola (1), Didymium squamulosum (1), Physarum compressum (3), P. gyrosum (1), P. pusillum (2), Stemonitis flavogenita (6) and an unidentified species of the order Physarales (2) were homothallic, with B. foliicola, P. compressum, and S. flavogenita being reported for the first time.

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Heterothallism and Homothallism: A Study of 27 Isolates of Didymium Iridis, a True Slime Mold

Cited by 17 publications

References 25 publications

RNA editing is absent in a single mitochondrial gene ofDidymium iridis

RNA editing is absent in a single mitochondrial gene ofDidymium iridis

Cytophotometric Measurement of Nuclear Dna in Seven Heterothallic Isolates of Didymium Iridis, a Myxomycete

Studies on the Mating Systems of Eleven Species of Myxomycetes

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