Heterotypic Smooth Muscle Cell/Endothelial Cell Interactions Differ between Species

Imegwu, Obi; Entersz, Ildiko; Graham, Alan M.; Nackman, Gary B.

doi:10.1006/jsre.2001.6165

Cited by 23 publications

(14 citation statements)

References 19 publications

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“…Calculations determined the average ratio of proliferating BPAECs in hetero- to homotypic pairs to be 0.58 ± 0.08 (small traps) and 0.58 ± 0.05 (large traps), in good agreement with the fit. We note that the proliferation decrease observed is in contrast to a study that measured proliferation of BPAECs cultured on a membrane with and without BPASMCs on the opposite side, which concluded that contact with the BPASMCs had no effect on BPAEC proliferation [28]. …”

Section: Resultscontrasting

confidence: 89%

“…To do so we used endothelial and smooth muscle cells that form the inner lining and interior structure of blood vessels, respectively, and are naturally in close proximity in vivo. Cell-cell interactions between these cell types have been studied previously in systems that cultured monolayers of each type on opposite sides of a porous membrane [27, 28], but this environment is unable to control the number of cells of the same type contacted, and heterotypic contacts are made by cytoplasmic projections extending through pores several microns in length. Our method allows for a much greater degree of contact between the cells, and eliminates the potentially confounding effects of contact with additional surrounding cells.…”

Section: Resultsmentioning

confidence: 99%

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Heterotypic cell pair co-culturing on patterned microarrays

et al. 2012

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We present a pair-wise co-culturing technique that creates large numbers of heterotypic cell pairs in patterned arrays. Lithographic patterning produces arrays with thousands of traps, each designed to accommodate only two cells and confine them at these sites for co-culturing. Two variants are introduced: a random seeding method that sediments a mixture of two cell types onto the array, and an approach that incorporates ferromagnetic thin films into the arrays and attracts cells that have been attached to ferromagnetic nanowires to the array sites through dipole interactions. The array technique includes the utilization of custom image analysis software that extracts data from multi-channel fluorescence images and records information about the cells in every trap, enabling the acquisition of accurate, high-statistics data. The applicability of the technique was demonstrated in experiments examining proliferation rates in pairs of bovine pulmonary artery endothelial and smooth muscle cells. Results demonstrated that heterotypic interactions favored smooth muscle cell proliferation while disfavoring endothelial cell proliferation. This is one example of a variety of cell-cell interactions that could be probed with this method.

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Section: Resultscontrasting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Heterotypic cell pair co-culturing on patterned microarrays

et al. 2012

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“…In view of the known heterogeneity of endothelial cells, [4][5][6][7][8][9][10][11][12][13][14][15] it would appear logical to study endothelial cells derived from the macular inner choroidal/choriocapillaris area of humans when studying the disease mechanisms of ARMD. While methods have been described for the isolation of human choroidal endothelial cells that contain a mixture of both inner and outer choroidal endothelial cells, 23 24 to date it has not been possible to reliably isolate human macular inner choroidal/choriocapillaris endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

“…[4][5][6][7][8][9][10][11][12][13][14][15] This suggests that the use of bovine, umbilical vein, or heterogeneous mixtures of human choroidal endothelial cells to study the pathophysiological mechanisms of ARMD [16][17][18][19] may not be truly representative.…”

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confidence: 99%

Isolation, culture, and characterisation of human macular inner choroidal microvascular endothelial cells

Browning

Gray²,

Amoaku³

2005

British Journal of Ophthalmology

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Aim: To develop a method for the reliable isolation of adult human macular inner choroidal endothelial cells (ICECs) and to subsequently characterise them for their expression of a range of endothelial cell associated surface markers. Method: Human ICECs were isolated after manual dissection of maculas from fresh human posterior segments. Following enzyme digestion to form a single cell suspension, the ICECs were isolated using anti-CD31 coated Dynabeads. The isolated cells were grown in culture and examined for typical endothelial cell morphology, surface expression of vWf, CD 31, CD 105, VEGF receptors 1 and 2, and expression of Eselectin after stimulation with TNF-a. The cells were also examined for their ability to form fenestrations and capillarylike tubes in Matrigel.

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“…33 Many factors have been reported to be able to influence the relationship between ECs and SMCs, such as species from which the cells are obtained, cell culture conditions, and cell confluence degree. 34,35 In this study, both EC-ECM and SMC-ECM stimulated the growth of ECs and SMCs, but SMC-ECM achieved a higher degree of stimulation compared to EC-ECM. This may be partly attributed to the larger amount of ECM secreted by SMCs, as reported by Bareille et al 36 However, our subsequent results indicate an unequivocal effect of ECM composition and not of quantity on cell adhesion and proliferation: ECs cultured for 7 days and SMCs cultured for 4 days on Ti disks deposited nearly equal amount of ECM; however, SMC-ECM still sustained higher cell adhesion (data not shown).…”

mentioning

confidence: 55%

Effect of Tissue Specificity on the Performance of Extracellular Matrix in Improving Endothelialization of Cardiovascular Implants

Yang

Zhu

et al. 2013

Tissue Engineering Part A

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Natural extracellular matrix (ECM) deposited in situ by cultured endothelial cells (ECs) has been proven effective in accelerating endothelialization of titanium (Ti) cardiovascular implants (CVIs) in our previous studies. In this study, the ECM deposited by smooth muscle cells (SMCs) was used in comparison to investigate the effects of tissue specificity of the ECM on the ability to accelerate endothelialization of CVIs. The results demonstrated that the ECM deposited by ECs and SMCs (EC-ECM, SMC-ECM, respectively) differed considerably in components and fibril morphology. Surface modification of Ti CVIs with both types of natural ECM was effective in improving their in vitro hemocompatibility and cytocompatibility simultaneously. However, the endothelialization of ECM-modified Ti CVIs in a canine model demonstrated a high tissue specificity of the ECM. Although the ECM deposited by SMCs (SMC-ECM) induced fewer platelet adhesion and sustained better growth and viability of ECs in vitro, its performance in accelerating in vivo endothelialization of Ti CVIs was extremely poor. In contrast, the ECM deposited by ECs (EC-ECM) led to complete endothelium formation in vivo.

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Heterotypic Smooth Muscle Cell/Endothelial Cell Interactions Differ between Species

Cited by 23 publications

References 19 publications

Heterotypic cell pair co-culturing on patterned microarrays

Heterotypic cell pair co-culturing on patterned microarrays

Isolation, culture, and characterisation of human macular inner choroidal microvascular endothelial cells

Effect of Tissue Specificity on the Performance of Extracellular Matrix in Improving Endothelialization of Cardiovascular Implants

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