Hevein-Like Antimicrobial Peptides Wamps: Structure–Function Relationship in Antifungal Activity and Sensitization of Plant Pathogenic Fungi to Tebuconazole by WAMP-2-Derived Peptides

Одинцова, Т. И.; Щербакова, Л. А.; Slezina, Marina P.; Pasechnik, Tatyana; Kartabaeva, Bakhyt; Dzhavakhiya, V. G.

doi:10.3390/ijms21217912

Cited by 28 publications

(29 citation statements)

References 73 publications

(125 reference statements)

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“…The obtained results showed F. oxysporum was significantly more sensitive to WAMP-N and WAMP-G2 than to other peptide fragments (Table 1). The results also indicated the entire WAMP-2, the activity of which was determined earlier under the same conditions [44], had a lower inhibitory effect than either of these more active fragments. WAMP-G1 possessed no antifungal activity towards this fungus; the minimum fungicidal concentration (MFC) exceeded 10,000 µg/mL.…”

Section: Enhancement Of the Fungicidal Effect Bysupporting

confidence: 63%

“…Microconidia of the pathogen were treated to each of the WAMP-2 fragments taken at three or four concentrations ranging between 50 and 200 (WAMP-N, WAMP-G2), 100 and 400 (WAMP-G1), or 50 and 400 (WAMP-C) µg/mL. The prior research showed these concentrations were marginally or low toxic for the fungus [44]. After the treatments, the rate of germinated conidia was determined, expressed as the percent relative to the total number of conidia counted (500 ones per treatment).…”

Section: Enhancement Of the Fungicidal Effect Bymentioning

confidence: 99%

“…We tested non-to low-toxic concentrations of four WAMP-2 fragments (WAMP-N, WAMP-G1, WAMPG2, WAMP-C) and showed they synergistically interacted with tebuconazole to drastically increase the sensitivity of target pathogens to Folicur ® EC 250 (25% tebuconazole). The synergism provided higher or complete inhibition of spore germination in these fungi at dosages of Folicur ® ineffective alone [44]. The research, reported here, explored the ability of the four above-mentioned WAMP-2 fragments to enhance the inhibitory effect of Folicur ® on the conidial germination in F. oxysporum f. sp.…”

Section: Introductionmentioning

confidence: 97%

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Fragments of a Wheat Hevein-Like Antimicrobial Peptide Augment the Inhibitory Effect of a Triazole Fungicide on Spore Germination of Fusarium oxysporum and Alternaria solani

et al. 2020

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There are increasing environmental risks associated with extensive use of fungicides for crop protection. Hence, the use of new approaches using natural plant defense mechanisms, including application of plant antimicrobial peptides (AMPs), is of great interest. Recently, we studied the structural–function relationships between antifungal activity and five hevein-like AMPs from the WAMP (wheat AMP) family of Triticum kiharae Dorof. et Migush. We first discovered that short peptides derived from the central, N-, and C-terminal regions of one of the WAMPs (WAMP-2) were able to augment the inhibitory effect of Folicur® EC 250, a triazole fungicide, on spore germination of the wheat pathogenic fungi, including Fusarium spp. and Alternaria alternata. In this research, we explored the ability of chemically synthesized WAMP-2-derived peptides for enhancing the sensitivity of two other Fusarium and Alternaria species, F. oxysporum and A. solani, causing wilt and early blight of tomato, respectively, to Folicur®. The synthesized WAMP-2-derived peptides synergistically interacted with the fungicide and significantly increased its efficacy, inhibiting conidial germination at much lower Folicur® concentrations than required for the same efficiency using the fungicide alone. The experiments on co-applications of some of WAMP-2-fragments and the fungicide on tomato leaves and seedlings, which confirmed the results obtained in vitro, are described.

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Section: Enhancement Of the Fungicidal Effect Bysupporting

confidence: 63%

Section: Enhancement Of the Fungicidal Effect Bymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Fragments of a Wheat Hevein-Like Antimicrobial Peptide Augment the Inhibitory Effect of a Triazole Fungicide on Spore Germination of Fusarium oxysporum and Alternaria solani

et al. 2020

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“…Many strategies have been proposed, among which peptides play an important role [ 17 ]. Interestingly, a similar target effect is a characteristic of AMPs with lectin-like functions (e.g., defensins [ 18 ] and hevein-like peptides [ 19 ], which can bind to N-glycosylated sites of the viral spike protein or the receptor, which ultimately prevents penetration of the virus into the host cell [ 20 , 21 ]. In general, as will be described below, AMPs have great potential for effective use in the therapy of coronavirus infection [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Amyloidogenic Activity of Peptides. Can Antimicrobial Peptides Be Used against SARS-CoV-2?

Kurpe

Grishin

Surin

et al. 2020

IJMS

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At present, much attention is paid to the use of antimicrobial peptides (AMPs) of natural and artificial origin to combat pathogens. AMPs have several points that determine their biological activity. We analyzed the structural properties of AMPs, as well as described their mechanism of action and impact on pathogenic bacteria and viruses. Recently published data on the development of new AMP drugs based on a combination of molecular design and genetic engineering approaches are presented. In this article, we have focused on information on the amyloidogenic properties of AMP. This review examines AMP development strategies from the perspective of the current high prevalence of antibiotic-resistant bacteria, and the potential prospects and challenges of using AMPs against infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

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“…The first research step to search for chemosensitizing compounds is the in vitro testing of the antifungal effect of putative sensitizers to determine their working concentration range and to make sure their toxicity is significantly lower than that of a fungicide used against the target pathogen. Recently, we found non-fungitoxic metabolites of plant origin, which enhanced the sensitivity of five cereal pathogens to one of DMI-fungicides [27]. Earlier, we also screened a range of microbial metabolites for sensitizing activity and briefly reported that a secondary metabolite of Penicillium citrinum, 6-demethylmevinolin (6-DMM) was the most promising as a putative sensitizer among them.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Sensitivity of Some Plant Pathogenic Fungi to 6-Demethylmevinolin, a Putative Natural Sensitizer Able to Help Overcoming the Fungicide Resistance of Plant Pathogens

et al. 2020

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Agricultural fungicides contaminate the environment and promote the spread of fungicide-resistant strains of pathogenic fungi. The enhancement of pathogen sensitivity to these pesticides using chemosensitizers allows the reducing of fungicide dosages without a decrease in their efficiency. Using Petri plate and microplate bioassays, 6-demethylmevinolin (6-DMM), a putative sensitizer of a microbial origin, was shown to affect both colony growth and conidial germination of Alternaria solani, A. alternata, Parastagonospora nodorum, Rhizoctonia solani, and four Fusarium species (F. avenaceum, F. culmorum, F. oxysporum, F. graminearum) forming a wheat root rot complex together with B. sorokiniana. Non- or marginally toxic 6-DMM concentrations suitable for sensitizing effect were determined by the probit analysis. The range of determined concentrations confirmed a possibility of using 6-DMM as a putative sensitizer for the whole complex of root rot agents, other cereal pathogens (A. alternata, P.nodorum), and some potato (R. solani, A. solani) and tomato (A. solani) pathogens. Despite the different sensitivities of the eight tested pathogens, 6-DMM lacked specificity to fungi and possessed a mild antimycotic activity that is typical of other known pathogen-sensitizing agents. The pilot evaluation of the 6-DMM sensitizing first confirmed a principal possibility of using it for the sensitization of B. sorokiniana and R. solani to triazole- and strobilurin-based fungicides, respectively.

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Hevein-Like Antimicrobial Peptides Wamps: Structure–Function Relationship in Antifungal Activity and Sensitization of Plant Pathogenic Fungi to Tebuconazole by WAMP-2-Derived Peptides

Abstract: Keywords: hevein-like antimicrobial peptides; antifungal activity; antifungal determinants; synergy; chemosensitization; tebuconazole; plant pathogenic fungi.

Cited by 28 publications

References 73 publications

Fragments of a Wheat Hevein-Like Antimicrobial Peptide Augment the Inhibitory Effect of a Triazole Fungicide on Spore Germination of Fusarium oxysporum and Alternaria solani

Fragments of a Wheat Hevein-Like Antimicrobial Peptide Augment the Inhibitory Effect of a Triazole Fungicide on Spore Germination of Fusarium oxysporum and Alternaria solani

Antimicrobial and Amyloidogenic Activity of Peptides. Can Antimicrobial Peptides Be Used against SARS-CoV-2?

Assessment of the Sensitivity of Some Plant Pathogenic Fungi to 6-Demethylmevinolin, a Putative Natural Sensitizer Able to Help Overcoming the Fungicide Resistance of Plant Pathogens

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