Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii

Wang, Dan; Li, Hong; Ma, Xiang; Tang, Yanqiong; Tang, Hongqian; Huang, Dong; Lin, Min; Li, Zhu

doi:10.3389/fmicb.2021.742114

Cited by 4 publications

(2 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies reported that Gram-negative bacteria, E. coli or Aeromonas veronii , devoid of Hfq are more sensitive to antibiotics [ 36 , 37 ]. Furthermore, deleting the hfq gene results in an increase in antibiotic accumulation [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…This pump is composed of three proteins: AcrB is an active drug transporter that functions with the proton-motive force across the IM; TolC forms an OM channel; and AcrA is a periplasmic linker [ 33 , 34 , 35 ]. Two studies have shown that Hfq plays a role in regulating acrB expression, and therefore, Hfq may contribute to antibiotic resistance [ 36 , 37 ]. Furthermore, Hfq modulates antibiotic import.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Role of the Bacterial Amyloid-like Hfq in Fluoroquinolone Fluxes

Turbant,

Esnouf,

Rosaz

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Due to their two-cell membranes, Gram-negative bacteria are particularly resistant to antibiotics. Recent investigations aimed at exploring new target proteins involved in Gram-negative bacteria adaptation helped to identify environmental changes encountered during infection. One of the most promising approaches in finding novel targets for antibacterial drugs consists of blocking noncoding RNA-based regulation using the protein cofactor, Hfq. Although Hfq is important in many bacterial pathogens, its involvement in antibiotics response is still unclear. Indeed, Hfq may mediate drug resistance by regulating the major efflux system in Escherichia coli, but it could also play a role in the influx of antibiotics. Here, using an imaging approach, we addressed this problem quantitatively at the single-cell level. More precisely, we analyzed how Hfq affects the dynamic influx and efflux of ciprofloxacin, an antibiotic from the group of fluoroquinolones that is used to treat bacterial infections. Our results indicated that the absence of either whole Hfq or its C-terminal domain resulted in a more effective accumulation of ciprofloxacin, irrespective of the presence of the functional AcrAB-TolC efflux pump. However, overproduction of the MicF small regulatory RNA, which reduces the efficiency of expression of the ompF gene (coding for a porin involved in antibiotics influx) in a Hfq-dependent manner, resulted in impaired accumulation of ciprofloxacin. These results led us to propose potential mechanisms of action of Hfq in the regulation of fluoroquinolone fluxes across the E. coli envelope.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Role of the Bacterial Amyloid-like Hfq in Fluoroquinolone Fluxes

Turbant,

Esnouf,

Rosaz

et al. 2023

Microorganisms

View full text Add to dashboard Cite

show abstract

Cryo soft X-ray tomography to explore Escherichia coli nucleoid remodeling by Hfq master regulator

Cossa

Wien²,

Groen³

et al. 2022

Journal of Structural Biology

View full text Add to dashboard Cite

Genome-wide DNA N6-methyladenosine in Aeromonas veronii and Helicobacter pylori

Zhao,

Ma,

Tang

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

Background DNA N6-methyladenosine (6mA), as an important epigenetic modification, widely exists in bacterial genomes and participates in the regulation of toxicity, antibiotic resistance, and antioxidant. With the continuous development of sequencing technology, more 6mA sites have been identified in bacterial genomes, but few studies have focused on the distribution characteristics of 6mA at the whole-genome level and its association with gene expression and function. Results This study conducted an in-depth analysis of the 6mA in the genomes of two pathogenic bacteria, Aeromonas veronii and Helicobacter pylori. The results showed that the 6mA was widely distributed in both strains. In A. veronii, 6mA sites were enriched at 3’ end of protein-coding genes, exhibiting a certain inhibitory effect on gene expression. Genes with low 6mA density were associated with cell motility. While in H. pylori, 6mA sites were enriched at 5’ end of protein-coding genes, potentially enhancing gene expression. Genes with low 6mA density were closely related to defense mechanism. Conclusions This study elucidated the distribution characteristics of 6mA in A. veronii and H. pylori, highlighting the effects of 6mA on gene expression and function. These findings provide valuable insights into the epigenetic regulation and functional characteristics of A. veronii and H. pylori.

show abstract

Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii

Cited by 4 publications

References 34 publications

Role of the Bacterial Amyloid-like Hfq in Fluoroquinolone Fluxes

Role of the Bacterial Amyloid-like Hfq in Fluoroquinolone Fluxes

Cryo soft X-ray tomography to explore Escherichia coli nucleoid remodeling by Hfq master regulator

Genome-wide DNA N6-methyladenosine in Aeromonas veronii and Helicobacter pylori

Contact Info

Product

Resources

About