“…Here, highly conserved point mutations in genes encoding mobile genetic elements and transposases were found Historically, net interactions (DA) have been the focus of evolutionary biologists (Chen et al, 2008;Cokol et al, 2017;Katzir et al, 2019;Zimmer et al, 2016) and ecologists (McCoy et al, 2012;Sitvarin & Rypstra, 2014;Sokol-Hessner & Schmitz, 2002) because of the difficulties in determining and describing higher-order emergent interactions accurately. Within the past 15 years, there has been substantial progress in determining and analyzing higher-order interactions (Beppler et al, 2016(Beppler et al, , 2017Cokol et al, 2017;Lozano-Huntelman et al, 2021;Palmer et al, 2015;Tekin et al, 2016;Tekin, White, et al, 2018;Yeh et al, 2009;Zimmer et al, 2016). In both net and emergent interactions, the effects of an interaction are not primarily due to the chemical interactions between the compounds but rather due to how each individual component affects the physiology of the cell and how those effects interact (Bollenbach, 2015;Bollenbach et al, 2009;Mason et al, 2017).…”