HIDRÓLISE ENZIMÁTICA, FERMENTAÇÃO E PRODUÇÃO DE BIOCOMBUSTÍVEIS ATRAVÉS DA COROA DE Ananas comosus

Silva, Jumelice dos S.; Malta, V. R. S.; Rocha, Martha Suzana Rodrigues dos Santos; Almeida, Renata Maria Rosas Garcia; Gomes, Márcia Andréa; Vicente, C.D.; Barbosa, Kledson Lopes

doi:10.21577/0100-4042.20170281

Cited by 3 publications

(4 citation statements)

References 24 publications

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“…The pH values ranging from 5.0 to 6.5 resulted in invertase production varying from 13.0 to 14.0 U/mL. Similar behaviour was observed of A. niger IBK1, when invertase production was higher in acidic pH range (4.0-6.0), with maximum invertase production occurring at pH=5.0 ( 16 ). Dinarvand et al .…”

Section: Resultssupporting

confidence: 57%

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Optimization of β-Fructofuranosidase Production from Agrowaste by Aspergillus carbonarius and Its Application in the Production of Inverted Sugar

Batista

Melo

Santos

et al. 2021

Food Technol. Biotechnol. (Online)

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Research background. Microbial β-fructofuranosidases are widely employed in food industry to produce inverted sugar or fructooligosaccharides. In this study, a newly isolated Aspergillus carbonarius PC-4 strain was used to optimize the β-fructofuranosidase production under a cost-effective process and the sucrose hydrolysis was evaluated to produce inverted sugars. Experimental approach. Optimization of nutritional components of culture medium was carried out using Simplex Lattice mixture design for 72 h and 120 h at 28 ˚C. One-Factor-at-a-time methodology was used to optimize the physicochemical parameters. Crude enzyme was used for sucrose hydrolysis at different concentrations. Results and conclusions. The optimized condition of enzyme production was pineapple crown waste (1.3 %, m/V) and yeast extract (0.3 %, m/V) for 72 h (9.4 U/mL), obtaining R² 91.85 %, R² adjusted 85.06 %, highest F value (13.52) and low p-value (0.003). One-factor-at-a-time used for optimizing the physicochemical conditions showed optimum temperature (20 ˚C), pH (5.5), agitation (180 rpm) and time-course (72 h) with an increase of 3.0-folds for enzyme production. The invertase-induced sucrose hydrolysis showed the maximum yield (3,451.7 µmols of reducing sugars) using 10 % of initial sucrose concentration. Higher sucrose concentrations caused inhibition of invertase activity, possibly due to saturation of substrate or formation of sucrose aggregates making it difficult for the enzyme to access sucrose molecules within the created clusters. So, a cost-effective method was developed for the invertase production using agroindustrial waste and the enzyme produced can be used efficiently for inverted sugar production at high sucrose concentration. Novelty and scientific contribution. This study presents an efficient utilization of pineapple crown wastes to produce invertase by a newly isolated Aspergillus carbonarius PC-4 strain. This enzyme exhibited a good potential for invert sugar production at high initial sucrose concentration, which is interesting for industrial applications.

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Section: Resultssupporting

confidence: 57%

“…Silva et al . ( 16 ) showed that pineapple crown is a potential carbon source, containing xylose and mostly glucose as constituents, and hence is an attractive residue for microbial growth. The predicted versus observed values revealed a good correlation between the model and experimental invertase production.…”

Section: Resultsmentioning

confidence: 99%

Optimization of β-Fructofuranosidase Production from Agrowaste by Aspergillus carbonarius and Its Application in the Production of Inverted Sugar

Batista

Melo

Santos

et al. 2021

Food Technol. Biotechnol. (Online)

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“…Os resultados estão apresentados na Tabela 1. A Figura 1 mostra que com 6h ocorre um decaimento na concentração de glicose e com 14h na concentração de xilose, demonstrando que a produção de etanol está associada ao consumo dos açúcares pela levedura (SILVA et al, 2018). A fermentação é o processo anterior à destilação e é a partir desta etapa que ocorre a degradação da glicose e outros nutrientes orgânicos em vários produtos, para geração de energia sob a forma de adenosina trifosfato, ou ATP (LEHNINGER et al, 2006).…”

Section: Resultados E Discussõesunclassified

Produção De Etanol 2g Com Casca De Coco Em Condições De Sacarificação E Fermentação Simultâneas

Peixoto¹,

Rocha²,

Almeida³

et al. 2019

Blucher Chemical Engineering Proceedings

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“…Ademais, essas condições para geração de combustível líquido fazem da matériaprima e do produto, fontes sustentáveis. [15][16][17] As indústrias de biocombustíveis têm empenhado grande esforço para estabelecer o melhor pré-tratamento da biomassa lignocelulósica para produção de etanol 2G. Exemplos de resíduos de biomassa utilizados para produção desse biocombustível são o bagaço e a palha da canade-açúcar, estudados por meio de diferentes prétratamentos para aumentar a oferta de celulose na obtenção do etanol 2G por meio dos seus açúcares fermentescíveis.…”

Section: Introductionunclassified

Pre-treatment of steam explosion, chemical and morphological characterization of sugarcane bagasse used for 2G Ethanol production

Barbosa¹,

Silva²,

Santos³

et al. 2020

Rev. Virtual Quim.

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Biofuels industries have made great efforts to establish the best pretreatment of lignocellulosic biomass for 2G-ethanol production. Therefore, this work, sugarcane bagasse (BCA), employed to the pre-treatment of steam explosion in an autoclave reactor at 120 °C. The chemical characterization performed by high performance liquid chromatography and morphological analyzes by spectroscopic techniques (FTIR and XRD). The evaluated pretreatment revealed changes in chemical and structural composition biomass with cellulose mass percentage of 37.51 and hemicellulose 21.32 in the untreated BCA for 30.82 cellulose and 17.14 of hemicellulose in the pre-treated BCA. The fractions cellulose and hemicelluloses decreased after pretreatment, suggesting generation other products as furfural, hydroxymethylfurfural, acetic acid and formic acid. The FTIR and XRD spectroscopy analyzes showed structural differences between the complexity of the untreated and pretreated biomass. Therefore, the results suggest that the pretreatment results in the breakdown of the biomass fibers. As indústrias de biocombustíveis têm empenhado grande esforço para estabelecer o melhor prétratamento da biomassa lignocelulósica para produção de etanol de segunda geração. Portanto, neste trabalho, o bagaço da cana-de-açúcar (BCA), foi submetido ao pré-tratamento de explosão a vapor em reator autoclave a 120 °C por 45 min. A caracterização química foi realizada por cromatografia líquida de alta eficiência e as análises morfológicas por técnicas espectroscópicas (FTIR e DRX). O pré-tratamento avaliado revelou mudanças na composição química e estrutural da biomassa com porcentagem mássica de celulose de 37,51 e hemiceluloses 21,32 no BCA não tratado para 30,82 de celulose e 17,14 de hemiceluloses no BCA pré-tratado. As frações de celulose e hemiceluloses diminuíram após o pré-tratamento, produzindo outros produtos como furfural, hidroximetilfurfural, ácido acético e ácido fórmico. As análises por espectroscopia FTIR e DRX indicaram diferenças estruturais entre a complexidade da biomassa não tratada e pré-tratada. Portanto, os resultados indicam que o pré-tratamento resulta em desorganização das fibras que constituem a biomassa.

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HIDRÓLISE ENZIMÁTICA, FERMENTAÇÃO E PRODUÇÃO DE BIOCOMBUSTÍVEIS ATRAVÉS DA COROA DE Ananas comosus

Cited by 3 publications

References 24 publications

Optimization of β-Fructofuranosidase Production from Agrowaste by Aspergillus carbonarius and Its Application in the Production of Inverted Sugar

Optimization of β-Fructofuranosidase Production from Agrowaste by Aspergillus carbonarius and Its Application in the Production of Inverted Sugar

Produção De Etanol 2g Com Casca De Coco Em Condições De Sacarificação E Fermentação Simultâneas

Pre-treatment of steam explosion, chemical and morphological characterization of sugarcane bagasse used for 2G Ethanol production

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