High affinity antibody based on a rationally designed hapten and development of a chemiluminescence enzyme immunoassay for quantification of Alternariol in fruit Juice, maize and flour

Yao, Chan-Yuan; Xu, Zhen-Lin; Wang, Hong; Zhu, Fan; Luo, Lin; Yang, Jin-Yi; Sun, Yuan; Lei, Hongtao; Tian, Yuanxin; Shen, Yu-Dong

doi:10.1016/j.foodchem.2018.12.127

Cited by 21 publications

(10 citation statements)

References 33 publications

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“…In contrast, ALb-type antibodies bound AOH with high affinity, but their recognition for AME was negligible-CR values were below 1% (Table 1). The IC 50 values to AOH of these specific antibodies were 1.2 nM, an affinity comparable to that of previously published polyclonal antibodies [11,13,16]. The position of the spacer arm in hapten ALa provided a closer mimic of the alkylated hydroxyl group of AME (C-9 position), whereas in hapten ALb the hydroxyl groups at C-7 and C-9 were unsubstituted, as in the molecule of AOH (Figure 1).…”

Section: Assessment Of the Immune Responsesupporting

confidence: 83%

“…Neither of these methods can be used to guide the position of attachment of the AOH molecular scaffold to the carrier proteins, so the antibodies were actually generated from an undefined mixture of functionalized haptens. More recently, Yao et al published an immunoassay for AOH using a carboxymethyl hapten to generate polyclonal antibodies [16]. Disappointingly, no synthetic details and spectrometric data of the prepared hapten were provided in that work.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development

Addante-Moya

Abad‐Somovilla

Abad‐Fuentes

et al. 2021

Toxins

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Immunochemical methods for mycotoxin analysis require antigens with well-defined structures and antibodies with outstanding binding properties. Immunoreagents for the mycotoxins alternariol and/or alternariol monomethyl ether have typically been obtained with chemically uncharacterized haptens, and antigen conjugates have most likely been prepared with mixtures of functionalized molecules. For the first time, total synthesis was performed, in the present study, to obtain two haptens with opposite linker attachment locations. The functionalized synthetic haptens were purified and deeply characterized by different spectrometric methods, allowing the preparation of bioconjugates with unequivocal structures. Direct and indirect competitive enzyme-linked immunosorbent assays, using homologous and heterologous conjugates, were employed to extensively evaluate the generated immunoreagents. Antibodies with high affinity were raised from conjugates of both haptens, and a structure-activity relationship between the synthetic haptens and the specificity of the generated antibodies could be established. These results pave the way for the development of novel highly sensitive immunoassays selective of one or two of these Alternaria mycotoxins.

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Section: Assessment Of the Immune Responsesupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development

Addante-Moya

Abad‐Somovilla

Abad‐Fuentes

et al. 2021

Toxins

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show abstract

“…The chemiluminescence-based enzyme immunoassay (CL-ELISA) improves the sensitivity of the experiment to a certain extent by enhancing the intensity of the signal value (Yao et al, 2019). The CL-ELISA resembles ELISA, in its basic principle except that the luminescent properties produced by the enzyme catalysing the substrate are different.…”

Section: Chemiluminescent Enzyme Immunoassay Strategies For Paesmentioning

confidence: 99%

Development of immunoassays for the determination of phthalates

Zhang

2020

Food and Agricultural Immunology

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The development of highly selective analytical methodologies to monitoring phthalates (phthalic acid esters, PAEs) is important for the safety of environment, food and equipment. As one of the most important and classical interactions in biological systems, antigen-antibody interactions constitute a highly specific chemical nature in biological systems, which inspire us to develop highly specific, highly selective and high-throughput immunoassays for monitoring PAEs in different fields. We here attempt to give an introduction of the recent development particularly focusing on the immunoassay based on the specific binding of antigen-antibody for detecting PAEs. We also present an overview of the potential applications of the antibody labelling strategies to immunoassays for PAE investigations. Finally, we present the major challenges and opportunities regarding the high selectivity and specificity of PAE analysis based on the antigen-antibody interaction. We believe that this review provides critical insights into highly selective PAE analysis and gives an understanding of immunoassays.

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“…Therefore, to obtain the anti-Van pAbs or monoclonal antibody from animals, it is necessary to couple the Van with a macromolecular carrier protein and then used for immunization. At present, BSA, OVA and KLH are the most common macromolecular carrier proteins for haptens conjugation in small molecule hazards immunoassay filed (Li et al, 2018;Yao et al, 2019). In this study, we chose KLH and BSA for the macromolecular carrier proteins, and prepared Van-KLH and Van-BSA conjugates, respectively.…”

Section: Preparation Of Van-klh and Van-bsa Conjugatesmentioning

confidence: 99%

Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination

Han

Dong

et al. 2019

Food and Agricultural Immunology

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Vancomycin (Van) is a common antibiotic that pollutes food, feed and ecological environment. In the present study, an anti-Van polyclonal antibodies (pAbs) and Eu 3+-labeled goat anti-rabbit IgG monoclonal antibody based indirect competitive timeresolved fluoroimmunoassay (IC-TRFIA) was established for ultrasensitive determination of Van, and its half-maximum inhibition concentrations (IC 50) was 13.74 ng/L and the limit of detection (LOD) was 1.07 ng/L. The IC-TRFIA showed strong crossreactivity (CRs) of 36.2% for norvancomycin, and less than 0.1% for polymyxin B, kanamycin and ampicillin. It was found that the IC-TRFIA for Van spiked in different milk powder samples determination were with good accuracy, stability and repeatability, and its recoveries are 83.0-97.9%, also with its coefficient of variations (CVs) are 3.0-12.9% in intra-assay and inter-assay. The results showed that the established IC-TRFIA was promising for ultrasensitive determination of Van in food samples. Highlights (1) Prepared high-quality Van-KLH/BSA conjugates. (2) Obtained high activity and purify anti-Van polyclonal antibodies. (3) Prepared high-quality Eu 3+-labelled goat anti-rabbit IgG monoclonal antibody. (4) Firstly established an IC-TRFIA for Van based on the anti-Van polyclonal antibodies and Eu 3+-labelled goat anti-rabbit IgG monoclonal antibody. (5) The IC-TRFIA can be used for ultrasensitive determination of Van in milk powder samples.

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High affinity antibody based on a rationally designed hapten and development of a chemiluminescence enzyme immunoassay for quantification of Alternariol in fruit Juice, maize and flour

Cited by 21 publications

References 33 publications

Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development

Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development

Development of immunoassays for the determination of phthalates

Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination

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