High-Affinity K+ Transporters from a Halophyte,<i>Sporobolus virginicus</i>, Mediate Both K+ and Na+ Transport in Transgenic Arabidopsis,<i>X. laevis</i>Oocytes and Yeast

Tada, Yuichi; Endo, Chikara; Katsuhara, Maki; Horie, Tomoaki; Shibasaka, Mineo; Nakahara, Yoshiki; Kurusu, Takamitsu

doi:10.1093/pcp/pcy202

Cited by 15 publications

(12 citation statements)

References 53 publications

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“…The transgenic lines showed elongated root growth on low-K + medium (containing 0.1 mM K + and 0.725 mM /Na + ), where root growth of WT plants is severely inhibited (Figure 6B,C). A similar phenotype was observed in Arabidopsis expressing K + /Na + symporters, SvHKT2s [16]. These results indicated that enhanced root growth of the transformants under low K + conditions was due to an increase in the ability to absorb Na + but not K + .…”

Section: Discussionsupporting

confidence: 72%

“…Similarly, altered expression of TaHKT2;1 in wheat affected Na + accumulation in the low-affinity range [15]. We also reported that SvHKT2;1 and SvHKT2;2 from a halophyte, Sporobolus virginicus, mediate both K + and Na + transport in transgenic Arabidopsis, Xenopus laevis oocytes, and yeast [16]. S. virginicus is a halophytic C 4 grass and shows a salinity tolerance up to 1.5 M NaCl [17].…”

Section: Introductionmentioning

confidence: 63%

“…There was a large variation in the expression level ( Figure 6A). We examined root growth of the transgenic lines on 0.1 mM K + medium, because expression of K + /Na + symporters, SvHKT2;1 and SvHKT2;2, in Arabidopsis resulted in enhanced root growth under K + -starved conditions in our previous study [16]. The SvHKT1;1 transgenic lines showed elongated root growth compared with WT plants, while the root growth of WT plants was severely inhibited ( Figure 6B,C).…”

Section: Constitutive Expression Of Svhkt1;1 In Wild-type Arabidopsismentioning

confidence: 90%

“…Similarly, RNA was extracted from 14-day-old Arabidopsis plants grown on 1/2 MS medium. Real-time qRT-PCR analysis was performed as previously reported [16] using primer sets for SvHKT1;1 and ubiquitin extension protein 5 (UBQ5), UBQ5F 5 -TGTGAAGGCGAAGATCCAAG-3 , and UBQ5R 5 -GAGACGGAGGACGAGATGAAG-3 as a reference.…”

Section: Real-time Qrt-pcrmentioning

confidence: 99%

“…The SvHKT1;1 cDNA was PCR-amplified using primers to produce the entry vector pENTER-SvHKT1;1, excised from the entry vectors using the restriction enzymes NotI and AscI, and then inserted into the NotI and AscI sites of pXBG-NA [16]. A mMESSAGE mMACHINE in vitro transcription kit (Thermo Fisher Scientific) was used to synthesize the capped-analogue RN.…”

Section: Functional Analysis Of Svhkt1;1 In X Laevis Oocytesmentioning

confidence: 99%

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Na+ Transporter SvHKT1;1 from a Halophytic Turf Grass Is Specifically Upregulated by High Na+ Concentration and Regulates Shoot Na+ Concentration

Kawakami

Imran

Katsuhara

et al. 2020

IJMS

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We characterized an Na+ transporter SvHKT1;1 from a halophytic turf grass, Sporobolus virginicus. SvHKT1;1 mediated inward and outward Na+ transport in Xenopus laevis oocytes and did not complement K+ transporter-defective mutant yeast. SvHKT1;1 did not complement athkt1;1 mutant Arabidopsis, suggesting its distinguishable function from other typical HKT1 transporters. The transcript was abundant in the shoots compared with the roots in S. virginicus and was upregulated by severe salt stress (500 mM NaCl), but not by lower stress. SvHKT1;1-expressing Arabidopsis lines showed higher shoot Na+ concentrations and lower salt tolerance than wild type (WT) plants under nonstress and salt stress conditions and showed higher Na+ uptake rate in roots at the early stage of salt treatment. These results suggested that constitutive expression of SvHKT1;1 enhanced Na+ uptake in root epidermal cells, followed by increased Na+ transport to shoots, which led to reduced salt tolerance. However, Na+ concentrations in phloem sap of the SvHKT1;1 lines were higher than those in WT plants under salt stress. Based on this result, together with the induction of the SvHKT1;1 transcription under high salinity stress, it was suggested that SvHKT1;1 plays a role in preventing excess shoot Na+ accumulation in S. virginicus.

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Section: Discussionsupporting

confidence: 72%