High-Affinity Peptide-Based Collagen Targeting Using Synthetic Phage Mimics: From Phage Display to Dendrimer Display

Helms, Brett A.; Reulen, Sanne W. A.; Nijhuis, Sebastiaan; Graaf-Heuvelmans, Peggy T.H.M. de; Merkx, Maarten; Meijer, E. W.

doi:10.1021/ja902285m

Cited by 74 publications

(108 citation statements)

References 23 publications

Supporting

Mentioning

101

Contrasting

Unclassified

Order By: Relevance

“…This phenomenon has a natural basis, vital to the function of binding proteins such as IgM with 10 binding sites (39) or the biological adhesion of many viruses (40), and even the extracellular matrix is polyvalent (41). Polyvalency is used frequently in the design of synthetic bioactive molecules to enhance their binding strength and has been used in the creation of bioactive peptides, organic molecules, carbohydrates, nucleotides, antibiotics, and phage mimics, among others (42)(43)(44)(45)(46). Polyvalency has even been explored for biological signaling initiated by receptor dimerization, as is the case for VEGF receptor signaling.…”

Section: Discussionmentioning

confidence: 99%

Supramolecular nanostructures that mimic VEGF as a strategy for ischemic tissue repair

Webber

Tongers

Newcomb

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

298

268

View full text Add to dashboard Cite

There is great demand for the development of novel therapies for ischemic cardiovascular disease, a leading cause of morbidity and mortality worldwide. We report here on the development of a completely synthetic cell-free therapy based on peptide amphiphile nanostructures designed to mimic the activity of VEGF, one of the most potent angiogenic signaling proteins. Following self-assembly of peptide amphiphiles, nanoscale filaments form that display on their surfaces a VEGF-mimetic peptide at high density. The VEGF-mimetic filaments were found to induce phosphorylation of VEGF receptors and promote proangiogenic behavior in endothelial cells, indicated by an enhancement in proliferation, survival, and migration in vitro. In a chicken embryo assay, these nanostructures elicited an angiogenic response in the host vasculature. When evaluated in a mouse hind-limb ischemia model, the nanofibers increased tissue perfusion, functional recovery, limb salvage, and treadmill endurance compared to controls, which included the VEGF-mimetic peptide alone. Immunohistological evidence also demonstrated an increase in the density of microcirculation in the ischemic hind limb, suggesting the mechanism of efficacy of this promising potential therapy is linked to the enhanced microcirculatory angiogenesis that results from treatment with these polyvalent VEGF-mimetic nanofibers.

show abstract

Section: Discussionmentioning

confidence: 99%

Supramolecular nanostructures that mimic VEGF as a strategy for ischemic tissue repair

Webber

Tongers

Newcomb

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

298

268

View full text Add to dashboard Cite

show abstract

“…1 Flory's early ideas were synthetically realized almost 30 years later when the divergent growth approach was introduced, followed in 1990 by the convergent strategy for the synthesis of dendrimers. [2][3][4][5] In recent years, the dendrimer field has expanded with numerous scientific publications discussing the use of these unique macromolecules in various applications such as multivalent affinity probes, 6 microelectronics, 7,8 and in light harvesting systems. 9 An ongoing challenge in developing applications for dendritic macromolecules is the elaboration of new methodologies for their synthesis.…”

Section: Introductionmentioning

confidence: 99%

Pushing the Limits for Thiol−Ene and CuAAC Reactions: Synthesis of a 6th Generation Dendrimer in a Single Day

et al. 2010

View full text Add to dashboard Cite

show abstract

“…orders of magnitude compared to monovalent formats (Helms et al 2009). Recently, our lab has described the capture of herpes simplex virus and anticancer proteins from soy whey, using multimeric peptide-coated magnetic particles Ran et al 2014).…”

Section: Multimeric Peptide Variantsmentioning

confidence: 99%

Advances in affinity ligand‐functionalized nanomaterials for biomagnetic separation

Fields

O'Mahony

et al. 2015

Biotech & Bioengineering

View full text Add to dashboard Cite

The downstream processing of proteins remains the most significant cost in protein production, and is largely attributed to rigorous chromatographic purification protocols, where the stringency of purity for biopharmaceutical products sometimes exceeds 99%. With an ever burgeoning biotechnology market, there is a constant demand for alternative purification methodologies, to ameliorate the dependence on chromatography, while still adhering to regulatory concerns over product purity and safety. In this article, we present an up-to-date view of bioseparation, with emphasis on magnetic separation and its potential application in the field. Additionally, we discuss the economic and performance benefits of synthetic ligands, in the form of peptides and miniaturized antibody fragments, compared to full-length antibodies. We propose that adoption of synthetic affinity ligands coupled with magnetic adsorbents, will play an important role in enabling sustainable bioprocessing in the future.

show abstract

High-Affinity Peptide-Based Collagen Targeting Using Synthetic Phage Mimics: From Phage Display to Dendrimer Display

Cited by 74 publications

References 23 publications

Supramolecular nanostructures that mimic VEGF as a strategy for ischemic tissue repair

Supramolecular nanostructures that mimic VEGF as a strategy for ischemic tissue repair

Pushing the Limits for Thiol−Ene and CuAAC Reactions: Synthesis of a 6th Generation Dendrimer in a Single Day

Advances in affinity ligand‐functionalized nanomaterials for biomagnetic separation

Contact Info

Product

Resources

About