High bone morphogenetic protein-15 level in follicular fluid is associated with high quality oocyte and subsequent embryonic development

Wu, Yanting; Tang, Li Li; Cai, Jie; Lu, Xiu-e; Xu, Jian; Zhu, Xiaoming; Luo, Qiong; Huang, He-feng

doi:10.1093/humrep/dem029

Cited by 118 publications

(98 citation statements)

References 33 publications

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“…The AAT levels in FF were measured by western blotting as described by Wu YT et al [13]. Briefly, 1.0 mL of FF was diluted in PBS and electrophoresed using 12 % sodium dodecylsulfate polyacrylamide gelelectrophoresis (SDS-PAGE).…”

Section: Analysis Of Alpha-1-antitrypsin (Aat) Complement Component mentioning

confidence: 99%

“…The composition of FF might influence and/or be implicated with oocyte quality. Some human FF proteins have been shown to be correlated with oocyte maturation, fertilization outcome and embryo development [13][14][15]. Therefore, identification of specifically altered protein levels in FF during COH cycles might be helpful in determining the influence of COH on follicular and oocyte development, and to predict IVF outcomes.…”

Section: Introductionmentioning

confidence: 99%

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Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

Zhang

et al. 2015

J Assist Reprod Genet

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Purpose To study the differences in protein expression profiles of follicular fluid (FF) between controlled ovarian hyperstimulation (COH) and natural ovulatory cycles. Methods Twelve infertile women undergoing in vitro fertilization and embryo transfer (IVF-ET), with matched clinical information, were retrospectively recruited in the IVF center of our university hospital, including six undergoing COH and another six with natural cycles. FF was sampled from dominant follicles with mature oocytes. Protein expression profiles in each FF sample were analyzed respectively using twodimensional gel electrophoresis. Differentially expressed proteins were identified using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) and validated by western blotting. Differentially expressed proteins were further analyzed using Ingenuity Pathway Analysis (IPA) software.Results Two proteins were downregulated and 11 proteins were upregulated (change ≥1.5-fold, P<0.05) in the COH group. We identified one down-egulated and seven upregulated proteins using MALDI-TOF MS. Four differentially expressed proteins, including transferrin, complement component C3 (C3), haptoglobin and alpha-1-antitrypsin (AAT), were further validated by rate nephelometry and western blotting analyses. The IPA analysis revealed a significant network involved in the humoral immune and inflammatory responses. Conclusions The eight differentially expressed proteins were related to immune and inflammatory responses in the ovary. Our results provide new insights into the influence of COH on follicular (spp) development and IVF outcomes.

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Section: Analysis Of Alpha-1-antitrypsin (Aat) Complement Component mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

Zhang

et al. 2015

J Assist Reprod Genet

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“…They compared FF BMP-15 levels of fertilized 60 oocytes with unfertilized 19 oocytes. FF BMP-15 level of fertilized oocytes were significantly higher than that of unfertilized oocytes [6]. Gueripel et al,observed increased BMP-15 immunostaining on ovarian tissue of mouse stimulated with gonadotropins [15].…”

Section: Discussionmentioning

confidence: 97%

“…Effect of AMH and BMP-15 on estradiol and progesteron production in primarycultured human luteinizing granulosa cells was reported [5]. BMP-15 level in follicular fluid has been demonstrated to associate with oocyte and embryo quality [6]. Researchers observed relation among cumulus cell BMP15 expression level and oocyte maturation, fertilization, and embryonic development [7].…”

Section: Introductionmentioning

confidence: 99%

What is the impact of serum and follicular fluid BMP-15 and AMH levels in ICSI-ET cycle outcomes?

Ilhan¹,

Özkan²,

Ekinci³

et al. 2017

Histol Cytol Embryol

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Objective: Bone morphogenetic protein-15 (BMP-15) and anti-Müllerian hormone (AMH) are the proteins functioning during ovarian follicular growth. We aimed to investigate the impact of serum and follicular fluid (FF) AMH and BMP-15 levels on outcomes of intracytoplasmic sperm injection-embryo transfer (ICSI-ET) cycles.Methods: This prospective, cross-sectional study was carried out with 82 patients attending ICSI-ET cycle. The women divided into two groups according to using either GnRH agonist (group1, N=38) or antagonist (group2, N=44) for pituitary down-regulation. Eight milliliter of serum samples were taken from the patients on the 3rd day of menstrual period (D3) and on the day of oocyte pick-up (OPU); and 8 mL of follicular fluid (FF) sample was collected during OPU. AMH and BMP-15 measurements were carried with ELISA method quantitatively. Results:The mean age and body mass index of population were 30.6 ± 5.2 years and 25.5±4.2 kg/m 2 respectively. The comparison of pregnancy rates between women according to having FF AMH (35% vs 42%) and FF BMP-15 (37.8% vs 40.5%) levels under and above of the mean values revealed no significant difference. Serum AMH levels showed negative correlation with fertilization rate (FR). D3 BMP-15 level showed positive correlation with progesterone level and endometrial thickness on the day of hCG injection.Conclusions: AMH and BMP-15 monitoring during ICSI-ET cycles for predicting cycle outcomes should be evaluated with further studies.

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“…6 The analysis of follicular fluid has recently been a matter of interest as a possible predictor of both oocyte developmental competence and the subsequent potential to achieve fertilization and embryo viability. [6][7][8][9][10] Moreover, the content of follicular fluid (FF) may also reflect the circulating biochemical environment and the changes in blood serum.…”

Section: Introductionmentioning

confidence: 99%

Does biochemical profile of follicular fluid in women undergoing assisted reproduction vary between the right and left ovaries?

Özdamar¹,

Gün

Ertuğrul

et al. 2015

Int J Reprod Contracept Obstet Gynecol

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High bone morphogenetic protein-15 level in follicular fluid is associated with high quality oocyte and subsequent embryonic development

Abstract: The present study demonstrates that the BMP-15 level in FF appears to be a potential factor in predicting oocyte quality and subsequent embryo development, and is correlated with E(2) level, which may additionally be a valuable predictor of oocyte fertilization.

Cited by 118 publications

References 33 publications

Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

What is the impact of serum and follicular fluid BMP-15 and AMH levels in ICSI-ET cycle outcomes?

Does biochemical profile of follicular fluid in women undergoing assisted reproduction vary between the right and left ovaries?

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